4E-BP1 Antibody | MyBioSource MBS150976 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

4E-BP1 Antibody

catalog :

MBS150976

quantity :

0.1 mg

price :

345 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunocytochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS150976

products type :

Antibody

products full name :

4E-BP1 Antibody

products short name :

4E-BP1

products name syn :

BP-1; 4EBP1; 4E-BP1; PHAS-I; Eukaryotic translation initiation factor 4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; eukaryotic translation initiation factor 4E binding protein 1

other names :

eukaryotic translation initiation factor 4E-binding protein 1; Eukaryotic translation initiation factor 4E-binding protein 1; eukaryotic translation initiation factor 4E-binding protein 1; eIF4E-binding protein 1; phosphorylated heat- and acid-stable protein regulated by insulin 1; eukaryotic translation initiation factor 4E binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I

products gene name :

EIF4EBP1

other gene names :

EIF4EBP1; EIF4EBP1; BP-1; 4EBP1; 4E-BP1; PHAS-I; 4E-BP1; eIF4E-binding protein 1; PHAS-I

uniprot entry name :

4EBP1_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse

sequence length :

118

purity :

4E-BP1 Antibody is affinity chromatography purified via peptide column.

form :

Liquid

concentration :

1 mg/mL

storage stability :

4E-BP1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

tested application :

ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC)

app notes :

4E-BP1 antibody can be used for the detection of 4E-BP1 by Western blot at 2.5 - 10 mug/mL. Antibody can also be used for immunocytochemistry starting at 2 mug/mL.

other info1 :

Conjugate: Unconjugated. Immunogen: 4E-BP1 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human 4E-BP1. Buffer: 4E-BP1 Antibody is supplied in PBS containing 0.02% sodium azide.

products description :

4E-BP1 Antibody: The translation of mRNA in eukaryotic cells is regulated by the presence of amino acids through multiple mechanisms. One such mechanism involves the evolutionarily conserved serine/threonine kinase TOR (Target of rapamycin, also known as mTOR), which regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. One downstream target of TOR is the eukaryotic initiation factor 4E binding protein 1 (4E-BP1) whose phosphorylation prevents its association with eIF4E, preferentially stimulating translation of mRNAs containing long, highly structured 5'-UTRs. Rapamycin inhibits TOR resulting in reduced cell growth and reduced rates of cell cycle and cell proliferationl, at least in part by inhibiting the activity of TOR towards 4E-BP1.

ncbi gi num :

4758258

ncbi acc num :

NP_004086

ncbi gb acc num :

NM_004095.3

uniprot acc num :

Q13541

ncbi mol weight :

12,580 Da

ncbi pathways :

AMPK Signaling Pathway (198868); AMPK Signaling Pathway (989139); AMPK Signaling Pathway (992181); Activation Of The MRNA Upon Binding Of The Cap-binding Complex And EIFs, And Subsequent Binding To 43S Pathway (105970); Acute Myeloid Leukemia Pathway (83117); Acute Myeloid Leukemia Pathway (529); Alpha6-Beta4 Integrin Signaling Pathway (198807); BDNF Signaling Pathway (712093); Cap-dependent Translation Initiation Pathway (105967); Choline Metabolism In Cancer Pathway (1059539)

ncbi summary :

This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq, Jul 2008]

uniprot summary :

4E-BP1: binds to eIF4E, preventing its assembly into the EIF4F complex and inhibiting cap-dependent translation. Phosphorylation of 4E-BP1 disrupts this binding, activating cap-dependent translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the PI3 kinase pathway. Protein type: Translation initiation; Translation. Chromosomal Location of Human Ortholog: 8p12. Cellular Component: nucleoplasm; protein complex; cytoplasm; cytosol. Molecular Function: protein binding; translation repressor activity; eukaryotic initiation factor 4E binding. Biological Process: negative regulation of translational initiation; cellular protein metabolic process; TOR signaling pathway; response to ethanol; translation; translational initiation; insulin receptor signaling pathway; gene expression; positive regulation of mitotic cell cycle; negative regulation of protein complex assembly; lung development; G1/S transition of mitotic cell cycle

size1 :

0.1 mg

price1 :

345 USD

more info or order :

MyBioSource product webpage