PD-1 Antibody | MyBioSource MBS150938 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

PD-1 Antibody

catalog :

MBS150938

quantity :

0.1 mg

price :

375 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunocytochemistry, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Immunohistochemistry of PD-1 in human brain tissue with PD-1 antibody at 5 g/mL.

image 2 :

Immunofluorescence of PD-1 in PD-1-transfected HEK293 cells with PD-1 antibody at 20 g/ml. Green: PD-1 Antibody (4067). Blue: DAPI staining

product information

catalog number :

MBS150938

products type :

Antibody

products full name :

PD-1 Antibody

products short name :

[PD-1]

products name syn :

[PD-1; PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1; PD1; Programmed cell death protein 1; Protein PD-1; programmed cell death 1]

other names :

[Programmed cell death protein 1; Programmed cell death protein 1; programmed cell death protein 1; protein PD-1; systemic lupus erythematosus susceptibility 2; programmed cell death 1; CD_antigen: CD279]

products gene name :

[PDCD1]

other gene names :

[PDCD1; PDCD1; PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1; PD1; Protein PD-1; hPD-1]

uniprot entry name :

PDCD1_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

288

purity :

PD-1 Antibody is affinity chromatography purified via peptide column.

form :

Liquid

concentration :

1 mg/mL

storage stability :

PD-1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

tested application :

ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin(IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)

app notes :

PD-1 antibody can be used for detection of PD-1 by Western blot at 1 ug/mL. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. !!User Note: Optimal dilutions for each application to be determined by the researcher.

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunofluorescence (IF)

image4 heading :

Immunocytochemistry (ICC)

image4 description :

Immunocytochemistry of PD-1 in PD-1-transfected HEK293 cells with PD-1 antibody at 10 g/ml.

other info1 :

Conjugate: Unconjugated. Immunogen: PD-1 antibody was raised against a 16 amino acid synthetic peptide from near the center of human PD-1. The Immunogen is located within amino acids 120-170 of PD-1. Buffer: PD-1 Antibody is supplied in PBS containing 0.02% sodium azide.

other info2 :

Predicted Molecular Weight: Predicted: 32kDa. Observed: 43kDa. Positive Control: MBS151681 THP-1 Cell Lysate

products description :

PD-1 Antibody: Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases.

ncbi gi num :

145559515

ncbi acc num :

Q15116.3

uniprot acc num :

Q15116

ncbi pathways :

Adaptive Immune System Pathway (366160); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Costimulation By The CD28 Family Pathway (119552); Immune System Pathway (106386); PD-1 Signaling Pathway (119557); T Cell Receptor Signaling Pathway (83080); T Cell Receptor Signaling Pathway (491)

ncbi summary :

This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. [provided by RefSeq, Jul 2008]

uniprot summary :

PDCD1: a single-pass type I membrane protein. A negative regulator of activated T cells, is upregulated in human and mouse exhausted T cells during persistent viral infection. A marker of angioimmunoblastic T-cell lymphoma and germinal center associated T cells. Protein type: Apoptosis; Membrane protein, integral. Chromosomal Location of Human Ortholog: 2q37.3. Cellular Component: integral to membrane; plasma membrane; external side of plasma membrane. Molecular Function: signal transducer activity; protein binding. Biological Process: apoptosis; multicellular organismal development; T cell costimulation; signal transduction; humoral immune response. Disease: Systemic Lupus Erythematosus

size1 :

0.1 mg

price1 :

375 USD

more info or order :

MyBioSource product webpage