antibody

SIRT2 Antibody

MBS150756

0.1 mg

345 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay

Antibody

SIRT2 Antibody

SIRT2

SIRT2; SIR2; SIR2L; SIR2L2; NAD-dependent protein deacetylase sirtuin-2; Regulatory protein SIR2 homolog 2; sirtuin (silent mating type information regulation 2 homolog) 2 (S. cerevisiae)

NAD-dependent protein deacetylase sirtuin-2 isoform 1; NAD-dependent protein deacetylase sirtuin-2; NAD-dependent protein deacetylase sirtuin-2; sirtuin type 2; SIR2-like protein 2; sir2-related protein type 2; silent information regulator 2; regulatory protein SIR2 homolog 2; NAD-dependent deacetylase sirtuin-2; sirtuin 2; Regulatory protein SIR2 homolog 2; SIR2-like protein 2

SIRT2

SIRT2; SIRT2; SIR2; SIR2L; SIR2L2; SIR2L; SIR2L2

SIR2_HUMAN

Polyclonal

IgG

Rabbit

Human, Mouse, Rat

389

SIRT2 Antibody is affinity chromatography purified via peptide column.

Liquid

1 mg/mL

SIRT2 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

SIRT2 antibody can be used for detection of SIRT2 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 5 mug/mL.

Conjugate: Unconjugated. Immunogen: SIRT2 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of the human SIRT2. Buffer: SIRT2 Antibody is supplied in PBS containing 0.02% sodium azide.

SIRT2 Antibody: The founding member of the sirtuin protein family was the silent information regulator 2 protein (Sir2p) of Saccharomyces cervisiae, an NAD+-dependent histone deacetylase (HDAC) that regulates chromatin silencing. The SIR2 family of genes are highly conserved from prokaryotes to eukaryotes. Mammals have seven homologs of Sir2p, SIRT1-7, which are involved in diverse processes ranging from transcriptional regulation, cell cycle progression and DNA-damage repair to aging. SIRT2 is a predominantly cytoplasmic protein that colocalizes with microtubules and can deacetylate alpha-tubulin and regulate progression through the cell cycle. Most Sirtuins are highly expressed in brain and testis, while Sirt2 expression is higher in fetal relative to adult brain. Recent studies on SIRT2 support the therapeutic utility of inhibitors for the treatment of neurodegenerative diseases such as Parkinson's disease.

13775600

NP_036369

NM_012237.3

Q8IXJ6

Predicted: 43 kDa. Observed: 52 kDa

Signaling Events Mediated By HDAC Class I Pathway (137997); Signaling Events Mediated By HDAC Class III Pathway (137972)

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Several transcript variants are resulted from alternative splicing of this gene. [provided by RefSeq, Jul 2010]

SIRT2: NAD-dependent protein deacetylase, which deacetylates internal lysines on histone and non-histone proteins. Deacetylates Lys-40 of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton. Deacetylates PCK1, opposing proteasomal degradation. Deacetylates Lys-310 of RELA. Interacts with HDAC6, suggesting that these proteins belong to a large complex that deacetylate the cytoskeleton. Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network. Inhibited by Sirtinol, A3 and M15 small molecules. Inhibited by nicotinamide. Belongs to the sirtuin family. Class I subfamily. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Deacetylase; EC 3.5.1.-. Chromosomal Location of Human Ortholog: 19q13. Cellular Component: centrosome; microtubule; lateral loop; paranode region of axon; perikaryon; chromosome; nuclear heterochromatin; cytosol; centriole; chromatin silencing complex; growth cone; perinuclear region of cytoplasm; cytoplasm; plasma membrane; spindle; midbody; nucleus; myelin sheath. Molecular Function: histone acetyltransferase binding; protein binding; ubiquitin binding; tubulin deacetylase activity; zinc ion binding; NAD-dependent histone deacetylase activity; histone deacetylase binding; protein deacetylase activity; beta-tubulin binding; NAD-dependent histone deacetylase activity (H4-K16 specific); chromatin binding; histone deacetylase activity; transcription factor binding; NAD+ ADP-ribosyltransferase activity. Biological Process: chromatin silencing at rDNA; proteasomal ubiquitin-dependent protein catabolic process; negative regulation of autophagy; phosphoinositide 3-kinase cascade; regulation of myelination; regulation of cell cycle; chromatin silencing; positive regulation of attachment of spindle microtubules to kinetochore; response to redox state; gene silencing; negative regulation of transcription from RNA polymerase II promoter; chromatin silencing at telomere; negative regulation of cell proliferation; substantia nigra development; meiotic cell cycle; protein amino acid deacetylation; cellular lipid catabolic process; protein kinase B signaling cascade; positive regulation of DNA binding; mitosis; positive regulation of meiosis; regulation of phosphorylation; transcription, DNA-dependent; regulation of exit from mitosis; hepatocyte growth factor receptor signaling pathway; histone deacetylation; negative regulation of striated muscle development; negative regulation of fat cell differentiation; myelination in the peripheral nervous system; protein amino acid ADP-ribosylation; cell division; positive regulation of cell division; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; autophagy; innate immune response; positive regulation of transcription from RNA polymerase II promoter; negative regulation of protein catabolic process; negative regulation of transcription, DNA-dependent

0.1 mg

345 USD