antibody

TFEB Antibody

MBS150693

0.1 mg

345 USD

polyclonal

rabbit

nonconjugated

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay

Antibody

TFEB Antibody

TFEB

TFEB; TCFEB; BHLHE35; ALPHATFEB; Transcription factor EB; Class E basic helix-loop-helix protein 35; bHLHe35; transcription factor EB

transcription factor EB isoform 1; Transcription factor EB; transcription factor EB; T-cell transcription factor EB; class E basic helix-loop-helix protein 35; transcription factor EB; Class E basic helix-loop-helix protein 35; bHLHe35

TFEB

TFEB; TFEB; TCFEB; BHLHE35; ALPHATFEB; BHLHE35; bHLHe35

TFEB_HUMAN

Polyclonal

IgG

Rabbit

Human

476

TFEB Antibody is affinity chromatography purified via peptide column.

Liquid

1 mg/mL

TFEB antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

TFEB antibody can be used for detection of TFEB by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 2.5 mug/mL.

Conjugate: Unconjugated. Immunogen: TFEB antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human TFEB. Buffer: TFEB Antibody is supplied in PBS containing 0.02% sodium azide.

TFEB Antibody: The Transcription factor EB (TFEB) is a member in the basic helix-loop-helix leucine zipper superfamily of transcription factors that is translocated in a subset of renal tumors. Recent studies have shown that lysosomal biogenesis is regulated by TFEB, which is in turn regulated by the mammalian target of rapamycin (mTOR) complex 1. Other evidence suggests that TFEB coordinates the major steps of the autophagic pathway by driving the expression of autophagy and lysosomal genes.

24307933

NP_009093

NM_007162.2

P19484

43,803 Da

TFEB: Transcription factor that specifically recognizes and binds E-box sequences (3 -CANNTG-5 ). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T- cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes and binds the CLEAR-box sequence (5 -GTCACGTGAC-3 ) present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta. Homodimer and heterodimer; with TFE3 or MITF. Belongs to the MiT/TFE family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: DNA-binding. Chromosomal Location of Human Ortholog: 6p21. Cellular Component: cytoplasm; nucleus. Molecular Function: protein dimerization activity; transcription factor activity. Biological Process: embryonic placenta development; regulation of transcription, DNA-dependent; transcription, DNA-dependent; lysosome organization and biogenesis; autophagy; positive regulation of transcription from RNA polymerase II promoter; positive regulation of autophagy; humoral immune response

0.1 mg

345 USD