antibody

Carabin Antibody

MBS150657

0.1 mg

345 USD

polyclonal

rabbit

nonconjugated

human, mouse

western blot, ELISA, enzyme immunoassay, immunohistochemistry - paraffin section

Antibody

Carabin Antibody

Carabin

Carabin; EPI64C; CARABIN; Carabin; TBC1 domain family member 10C; TBC1 domain family, member 10C

carabin isoform 1; Carabin; carabin; TBC1 domain family, member 10C; TBC1 domain family member 10C

TBC1D10C

TBC1D10C; TBC1D10C; EPI64C; CARABIN

TB10C_HUMAN

Polyclonal

IgG

Rabbit

Human, Mouse

446

Carabin Antibody is affinity chromatography purified via peptide column.

Liquid

1 mg/mL

Carabin antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Carabin antibody can be used for detection of Carabin by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. Optimal dilutions for each application to be determined by the researcher. Positive Control: . MBS151632 Daudi Cell Lysate. MBS151674 Human Spleen Tissue Lysate.

Conjugate: Unconjugated. Immunogen: Carabin antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human Carabin. Buffer: Carabin Antibody is supplied in PBS containing 0.02% sodium azide.

Carabin Antibody: Antigen binding by the T-cell receptor (TCR) is one of the critical first steps in the immune response, triggering a cascade of signaling pathways that ultimately lead to T-cell activation. Screening a yeast two-hybrid screen of a human T-cell cDNA library with calcineurin, a protein phosphatase involved in multiple signaling pathways including T-cell activation, resulted in the identification of Carabin, a member of the TBC1 domain family of proteins, as a calcineurin-binding protein. Unlike other members of the TBC1 domain protein family which are thought to have a role in regulating cell growth and differentiation, further experiments demonstrated that Carabin is part of a negative regulatory loop for the intracellular TCR signaling pathway as well as an inhibitor of the Ras signaling pathway, suggesting that Carabin may also mediate crosstalk between calcineurin and Ras. Carabin antibody does not recognize TBC1D10A or TBC1D10B. Carabin is known to exist in multiple isoforms.

38348348

NP_940919

NM_198517.3

Q8IV04

32,887 Da

The protein encoded by this gene has an N-terminal Rab-GTPase domain and a binding site at the C-terminus for calcineurin, and is an inhibitor of both the Ras signaling pathway and calcineurin, a phosphatase regulated by calcium and calmodulin. Genes encoding similar proteins are located on chromosomes 16 and 22. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2013]

TBC1D10C: Inhibits the Ras signaling pathway through its intrinsic Ras GTPase-activating protein (GAP) activity. Acts as a negative feedback inhibitor of the calcineurin signaling pathway that also mediates crosstalk between calcineurin and Ras. Protein type: GAPs; GAPs, Rab. Chromosomal Location of Human Ortholog: 11q13.2. Cellular Component: filopodium membrane; membrane; plasma membrane. Biological Process: negative regulation of B cell activation; negative regulation of NFAT protein import into nucleus; retrograde transport, endosome to Golgi

0.1 mg

345 USD