antibody

Bmf Antibody

MBS150512

0.1 mg

345 USD

polyclonal

rabbit

nonconjugated

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay

Antibody

Bmf Antibody

Bmf

Bmf; Bcl-2-modifying factor; Bcl2 modifying factor

bcl-2-modifying factor isoform bmf-1; Bcl-2-modifying factor; bcl-2-modifying factor; Bcl2 modifying factor

BMF

BMF; BMF

BMF_HUMAN

Polyclonal

IgG

Rabbit

Human

184

Bmf Antibody is affinity chromatography purified via peptide column.

Liquid

1 mg/mL

Bmf antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Bmf antibody can be used for detection of Bmf by Western blot at 2.5 and 5 mug/mL. A band at approximately 25 kDa can be detected. Antibody can also be used for immunohistochemistry starting at 10 mug/mL. For immunofluorescence start at 10 mug/mL.

Conjugate: Unconjugated. Immunogen: Bmf antibody was raised with a synthetic peptide corresponding to 14 amino acids near the carboxy terminus of human Bmf. Buffer: Bmf Antibody is supplied in PBS containing 0.02% sodium azide.

Bmf Antibody: Apoptosis is related to many diseases and development. Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3-only proteins, including Bad, Bid, Bik, Hrk, Bim, Noxa, and PUMA, form a growing subclass of the Bcl-2 family. A novel BH3-only protein was recently identified in human and mouse and designated Bmf (for Bcl-2-modifing factor). The BH3 domain in Bmf is required both for binding to Bcl-2 proteins and for triggering apoptosis. In healthy cells, Bmf associates with the dynein light chain 2 (DLC2) component of the myosin V motors and is sequestered by the cell's actin cytoskeleton. Disruption of the actin cytoskeleton, either by depolymerization of actin filaments or by detachment of cells from the extracellular matrix, triggers release and activation of Bmf, initiating the downstream apoptotic program. Bmf is constitutively expressed in many tissues.

15723378

NP_277038

NM_033503.3

Q96LC9

25 kDa

Activation Of BH3-only Proteins Pathway 105658!!Activation Of BMF And Translocation To Mitochondria Pathway 105663!!Androgen Receptor Signaling Pathway 198806!!Apoptosis Pathway 105648!!Apoptosis Modulation And Signaling Pathway 198822!!BH3-only Proteins Associate With And Inactivate Anti-apoptotic BCL-2 Members Pathway 105664!!Intrinsic Pathway For Apoptosis 105656!!MicroRNAs In Cancer Pathway 852705!!MicroRNAs In Cancer Pathway 852928!!Programmed Cell Death Pathway 1127510

The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein contains a single BCL2 homology domain 3 (BH3), and has been shown to bind BCL2 proteins and function as an apoptotic activator. This protein is found to be sequestered to myosin V motors by its association with dynein light chain 2, which may be important for sensing intracellular damage and triggering apoptosis. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

0.1 mg

345 USD