antibody

SCRAPPER Antibody

MBS150412

0.1 mg

345 USD

polyclonal

rabbit

nonconjugated

human, mouse

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay

Antibody

SCRAPPER Antibody

SCRAPPER

SCRAPPER; Fbl2; Fbl20; FBL2; F-box and leucine-rich repeat protein 20; F-box and leucine-rich repeat protein 20

F-box/LRR-repeat protein 20 isoform 1; F-box/LRR-repeat protein 20; F-box/LRR-repeat protein 20; F-box protein FBL2; F-box and leucine-rich repeat protein 20; F-box and leucine-rich repeat protein 20; F-box/LRR-repeat protein 2-like

FBXL20

FBXL20; FBXL20; Fbl2; Fbl20; FBL2

FXL20_HUMAN

Polyclonal

IgG

Rabbit

Human, Mouse, Rat

436

SCRAPPER Antibody is affinity chromatography purified via peptide column.

Liquid

1 mg/mL

SCRAPPER antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

SCRAPPER antibody can be used for detection of SCRAPPER by Western blot at 0.5 - 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.

Conjugate: Unconjugated. Immunogen: SCRAPPER antibody was raised against a 18 amino acid synthetic peptide near the amino terminus of the human SCRAPPER. Buffer: SCRAPPER Antibody is supplied in PBS containing 0.02% sodium azide.

SCRAPPER Antibody: Members of the F-box protein family, such as Scrapper, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1, cullin, and F-box proteins, act as protein-ubiquitin ligases. Scrapper is selectively expressed in the brain, broadly expressed within the mouse CNS and is abundant at presynaptic membrane. Scrapper has orthologs in C. elegans, D. melanogaster, and mammals which suggests that it might function as an important membrane-localized E3 ligase in various species. Scrapper is a major presynaptic E3 ubiquitin ligase that acts through RIM1a via degradation and the ubiquitin-proteasome-system (UPS) pathway to critically regulate synaptic transmission. This identifies protein degradation as a mechanism for holding synaptic communication in check.

27734755

NP_116264

NM_032875.2

Q96IG2

44,980 Da

Members of the F-box protein family, such as FBXL20, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM, Mar 2008]

0.1 mg

345 USD