GLS2 Antibody | MyBioSource MBS150300 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

GLS2 Antibody

catalog :

MBS150300

quantity :

0.1 mg

price :

345 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS150300

products type :

Antibody

products full name :

GLS2 Antibody

products short name :

GLS2

products name syn :

GLS2; GA; GLS; LGA; hLGA; GA; Glutaminase liver isoform; mitochondrial; L-glutaminase; glutaminase 2 (liver, mitochondrial)

other names :

glutaminase liver isoform, mitochondrial isoform 1; Glutaminase liver isoform, mitochondrial; glutaminase liver isoform, mitochondrial; glutaminase I; breast cell glutaminase; L-glutamine amidohydrolase; phosphate-activated glutaminase; phosphate-dependent glutaminase; glutaminase 2 (liver, mitochondrial); L-glutaminase; L-glutamine amidohydrolase

products gene name :

GLS2

other gene names :

GLS2; GLS2; GA; GLS; LGA; hLGA; GA; GLS

uniprot entry name :

GLSL_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

602

specificity :

Multiple isoforms of GLS2 are known to exist.

purity :

GLS2 Antibody is affinity chromatography purified via peptide column.

form :

Liquid

concentration :

1 mg/mL

storage stability :

GLS2 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

tested application :

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

app notes :

GLS2 antibody can be used for detection of GLS2 by Western blot at 0.5 - 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.

other info1 :

Conjugate: Unconjugated. Immunogen: GLS2 antibody was raised against an 18 amino acid synthetic peptide near the center terminus of human GLS2. Buffer: GLS2 Antibody is supplied in PBS containing 0.02% sodium azide.

products description :

GLS2 Antibody: Phosphate-activated glutaminase, also known as Glutaminase 2 (GLS2), was initially isolated from rat liver, although it has been shown to be expressed in other tissues. Like the functionally similar, larger kidney glutaminase, GLS2 catalyzes the hydrolysis of glutamine to stoichiometric amounts of glutamate and ammonia. Expression of GLS2 is increased by p53 under both stressed and nonstressed conditions, resulting in increased levels of glutamate and alpha-ketoglutarate, which in turn results in enhanced mitochondrial respiration and ATP generation. GLS2 also regulates antioxidant defense function in cells by increasing reduced glutathione levels and decreasing ROS-levels, suggesting that GLS2 acts as a mediator of p53's role in antioxidant defense in addition to its role in energy metabolism.

ncbi gi num :

20336214

ncbi acc num :

NP_037399

ncbi gb acc num :

NM_013267.3

uniprot acc num :

Q9UI32

ncbi mol weight :

Predicted: 36; 55; 62; 66 kDa. Observed: 55; 62 kDa

ncbi pathways :

Alanine, Aspartate And Glutamate Metabolism Pathway (101142); Alanine, Aspartate And Glutamate Metabolism Pathway (100063); Amino Acid Synthesis And Interconversion (transamination) Pathway (106173); Arginine And Proline Metabolism Pathway (82957); Arginine And Proline Metabolism Pathway (323); Central Carbon Metabolism In Cancer Pathway (1059538); Central Carbon Metabolism In Cancer Pathway (1084231); D-Glutamine And D-glutamate Metabolism Pathway (82971); D-Glutamine And D-glutamate Metabolism Pathway (340); GABAergic Synapse Pathway (377263)

ncbi summary :

The protein encoded by this gene is a mitochondrial phosphate-activated glutaminase that catalyzes the hydrolysis of glutamine to stoichiometric amounts of glutamate and ammonia. Originally thought to be liver-specific, this protein has been found in other tissues as well. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, Jul 2013]

uniprot summary :

GLS2: Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production. May play a role in preventing tumor proliferation. Belongs to the glutaminase family. Protein type: Mitochondrial; Energy Metabolism - nitrogen; Amino Acid Metabolism - alanine, aspartate and glutamate; Other Amino Acids Metabolism - D-Glutamine and D-glutamate; EC 3.5.1.2; Hydrolase; Amino Acid Metabolism - arginine and proline. Chromosomal Location of Human Ortholog: 12q13.3. Cellular Component: mitochondrion; mitochondrial matrix. Molecular Function: protein binding; glutaminase activity. Biological Process: amino acid metabolic process; regulation of apoptosis; synaptic transmission; glutamate secretion; neurotransmitter secretion; glutamine metabolic process; amino acid biosynthetic process

size1 :

0.1 mg

price1 :

345 USD

more info or order :

MyBioSource product webpage