APC4 Antibody | MyBioSource MBS150295 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

APC4 Antibody

catalog :

MBS150295

quantity :

0.1 mg

price :

345 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS150295

products type :

Antibody

products full name :

APC4 Antibody

products short name :

APC4

products name syn :

APC4; APC4; APC4; Anaphase-promoting complex subunit 4; Cyclosome subunit 4; anaphase promoting complex subunit 4

other names :

Anaphase-promoting complex subunit 4; Anaphase-promoting complex subunit 4; anaphase-promoting complex subunit 4; cyclosome subunit 4; anaphase promoting complex subunit 4; Cyclosome subunit 4

products gene name :

ANAPC4

other gene names :

ANAPC4; ANAPC4; APC4; APC4; APC4

uniprot entry name :

APC4_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

808

purity :

APC4 Antibody is affinity chromatography purified via peptide column.

form :

Liquid

concentration :

1 mg/mL

storage stability :

APC4 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

tested application :

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

app notes :

APC4 antibody can be used for detection of APC4 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL.

other info1 :

Conjugate: Unconjugated. Immunogen: APC4 antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human APC4. Buffer: APC4 Antibody is supplied in PBS containing 0.02% sodium azide.

products description :

APC4 Antibody: Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC4 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. While little is known of APC4, it is thought that APC4 associates with other APC/C components APC1, APC5, and CDC23 interdependently, such that loss of any one subunit reduces binding between the remaining three.

ncbi gi num :

205371737

ncbi acc num :

Q9UJX5.2

uniprot acc num :

Q9UJX5

ncbi mol weight :

92,216 Da

ncbi pathways :

APC-Cdc20 Mediated Degradation Of Nek2A Pathway (1127551); APC/C Complex Pathway (413481); APC/C Complex Pathway (468395); APC/C-mediated Degradation Of Cell Cycle Proteins Pathway (105825); APC/C:Cdc20 Mediated Degradation Of Cyclin B Pathway (105832); APC/C:Cdc20 Mediated Degradation Of Securin Pathway (105833); APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway (105831); APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway (105835); APC:Cdc20 Mediated Degradation Of Cell Cycle Proteins Prior To Satisfation Of The Cell Cycle Checkpoint Pathway (1127549); Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway (105829)

ncbi summary :

A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eight subunits. The composition of the APC is highly conserved in organisms from yeast to humans. The exact function of this gene product is not known. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]

uniprot summary :

APC4: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of Lys-11 -linked polyubiquitin chains and, to a lower extent, the formation of Lys-48 - and Lys-63 -linked polyubiquitin chains. Belongs to the APC4 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Ubiquitin conjugating system. Chromosomal Location of Human Ortholog: 4p15.2. Cellular Component: nucleoplasm; anaphase-promoting complex; nucleus; cytosol. Molecular Function: protein binding; ubiquitin-protein ligase activity; protein phosphatase binding. Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; mitosis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; cell division; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle spindle assembly checkpoint; mitotic cell cycle

size1 :

0.1 mg

price1 :

345 USD

more info or order :

MyBioSource product webpage