antibody

Aak1 Antibody

MBS150292

0.1 mg

345 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, ELISA, enzyme immunoassay

Antibody

Aak1 Antibody

Aak1

KIAA1048; AP2-associated protein kinase 1; Adaptor-associated kinase 1; AP2 associated kinase 1

AP2-associated protein kinase 1; AP2-associated protein kinase 1; AP2-associated protein kinase 1; adaptor-associated kinase 1; AP2 associated kinase 1; Adaptor-associated kinase 1

AAK1

AAK1; AAK1; KIAA1048

AAK1_HUMAN

Polyclonal

IgG

Rabbit

Human, Mouse, Rat

961

Aak1 Antibody is affinity chromatography purified via peptide column.

Liquid

1 mg/mL

Aak1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

ELISA (EIA), Western Blot (WB)

Aak1 antibody can be used for detection of Aak1 by Western blot at 1 - 2 mug/mL.

Conjugate: Unconjugated. Immunogen: Aak1 antibody was raised against a 18 amino acid synthetic peptide near the carboxy terminus of the human Aak1. Buffer: Aak1 Antibody is supplied in PBS containing 0.02% sodium azide.

Aak1 Antibody: AP2-associated protein kinase 1 (Aak1) is a member of the Ark1/Prk1 subfamily of Ser/Thr protein kinases that are thought to regulate endocytosis by phosphorylating the accessory endocytic components. Aak1 interacts with and phosphorylates the mu2 subunit of the AP-2 complex, which promotes binding of the AP-2 to tyrosine based (Yxxphi) internalization motif-containing receptors and subsequent receptor endocytosis. At least two isoforms of Aak1 are known to exist; the longer isoform contains an extended carboxy-terminus that contains an additional clathrin-binding domain. Overexpression of this long isoform or Aak1 depletion by RNA interference impairs transferrin recycling from the early/sorting endosome, suggesting that Aak1 functions at multiple steps of the endosomal pathway by regulating transferrin internalization and its recycling back to the plasma membrane.

148277037

NP_055726

NM_014911.3

Q2M2I8

93,578 Da

Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. This gene encodes a member of the SNF1 subfamily of Ser/Thr protein kinases. The protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]

AAK1: an NAK kinase. Plays a regulatory role in clathrin-mediated endocytosis, including the regulation of fatty acid synthesis and cholesterol synthesis. Protein type: EC 2.7.11.1; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Other group; NAK family. Chromosomal Location of Human Ortholog: 2p14. Cellular Component: extrinsic to plasma membrane; clathrin-coated vesicle; leading edge; terminal button; coated pit. Molecular Function: protein serine/threonine kinase activity; protein binding; Notch binding; ATP binding. Biological Process: regulation of protein localization; protein stabilization; protein amino acid autophosphorylation; endocytosis; protein amino acid phosphorylation; positive regulation of Notch signaling pathway

0.1 mg

345 USD