antibody

PKR Antibody

MBS150276

0.1 mg

345 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay

Antibody

PKR Antibody

PKR

PKR; PKR; PRKR; EIF2AK1; PKR; Interferon-induced; double-stranded RNA-activated protein kinase; Eukaryotic translation initiation factor 2-alpha kinase 2; eIF-2A protein kinase 2; eukaryotic translation initiation factor 2-alpha kinase 2

interferon-inducible double-stranded RNA-dependent protein kinase; Interferon-induced, double-stranded RNA-activated protein kinase; interferon-induced, double-stranded RNA-activated protein kinase; p68 kinase; protein kinase R; eIF-2A protein kinase 2; P1/eIF-2A protein kinase; tyrosine-protein kinase EIF2AK2; interferon-inducible elF2alpha kinase; double stranded RNA activated protein kinase; protein phosphatase 1, regulatory subunit 83; protein kinase, interferon-inducible double stranded RNA dependent; eukaryotic translation initiation factor 2-alpha kinase 2; Eukaryotic translation initiation factor 2-alpha kinase 2

EIF2AK2

EIF2AK2; EIF2AK2; PKR; PRKR; EIF2AK1; PPP1R83; eIF-2A protein kinase 2; PKR; Protein kinase R

E2AK2_HUMAN

Polyclonal

IgG

Rabbit

Human, Mouse, Rat

510

PKR Antibody is affinity chromatography purified via peptide column.

Liquid

1 mg/mL

PKR antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

PKR antibody can be used for detection of PKR by Western blot at 0.5 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.

Conjugate: Unconjugated. Immunogen: PKR antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human PKR. Buffer: PKR Antibody is supplied in PBS containing 0.02% sodium azide.

PKR Antibody: The interferon-inducible, double-stranded RNA (dsRNA)-dependent protein kinase PKR is a member of the eukaryotic initiation factor-2 alpha (eIF2-alpha) kinase family, possessing serine-threonine kinase activity and two dsRNA-binding motifs that acts as part of the innate immune system. Upon binding dsRNA, PKR undergoes a conformational change leading to its activation and its phosphorylation of the translation factor eIF2, resulting in a general shutdown of protein synthesis and induction of apoptosis through upregulation of caspase-8 and capsase-9 activity in order to prevent the production of more viruses. To evade the antiviral effects of PKR, viruses have evolved multiple mechanisms, such as the inhibition of PKR by the non-structural protein (NS1) of the influenza virus. More recently, PKR has been implicated in several neurodegenerative diseases including Alzheimer, Huntington, and amyotrophic lateral sclerosis.

31747519

AAP57628

P19525

57,391 Da

Antiviral Mechanism By IFN-stimulated Genes Pathway 530760!!Ceramide Signaling Pathway 138023!!Cytokine Signaling In Immune System Pathway 366171!!Disease Pathway 530764!!Epstein-Barr Virus Infection Pathway 585562!!Epstein-Barr Virus Infection Pathway 587115!!Hepatitis C Pathway 173973!!Hepatitis C Pathway 173907!!Herpes Simplex Infection Pathway 377873!!Herpes Simplex Infection Pathway 377865

The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

0.1 mg

345 USD