antibody

SIRP alpha Antibody

MBS150220

0.1 mg

345 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, ELISA, immunocytochemistry, enzyme immunoassay

Antibody

SIRP alpha Antibody

SIRP alpha

SIRP alpha; BIT; MFR; P84; SIRP; MYD-1; SHPS1; CD172A; PTPNS1; BIT; MYD1; Tyrosine-protein phosphatase non-receptor type substrate 1; Brain Ig-like molecule with tyrosine-based activation motifs; SHP substrate 1; signal-regulatory protein alpha

tyrosine-protein phosphatase non-receptor type substrate 1; Tyrosine-protein phosphatase non-receptor type substrate 1; tyrosine-protein phosphatase non-receptor type substrate 1; myd-1 antigen; inhibitory receptor SHPS-1; macrophage fusion receptor; CD172 antigen-like family member A; tyrosine phosphatase SHP substrate 1; brain-immunoglobulin-like molecule with tyrosine-based activation motifs; signal-regulatory protein alpha; Brain Ig-like molecule with tyrosine-based activation motifs; Bit; CD172 antigen-like family member A; Inhibitory receptor SHPS-1; Macrophage fusion receptor; MyD-1 antigen; Signal-regulatory protein alpha-1; Sirp-alpha-1; Signal-regulatory protein alpha-2; Sirp-alpha-2; Signal-regulatory protein alpha-3; Sirp-alpha-3; p84; CD_antigen: CD172a

SIRPA

SIRPA; SIRPA; BIT; MFR; P84; SIRP; MYD-1; SHPS1; CD172A; PTPNS1; BIT; MFR; MYD1; PTPNS1; SHPS1; SIRP; SHP substrate 1; SHPS-1; Bit; Sirp-alpha-1; Sirp-alpha-2; Sirp-alpha-3

SHPS1_HUMAN

Polyclonal

IgG

Rabbit

Human, Mouse, Rat

504

Recognizes SIRP alpha 1, 2 and 3.

SIRP alpha Antibody is affinity chromatography purified via peptide column.

Liquid

1 mg/mL

SIRP alpha antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC)

SIRP alpha antibody can be used for Western blot at 0.5 - 1 mug/mL. 75 - 110 kDa bands should be detected. Antibody can also be used for immunocytochemistry starting at 1 mug/mL.

Conjugate: Unconjugated. Immunogen: SIRP alpha antibody was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human SIRP alpha 1. Buffer: SIRP alpha Antibody is supplied in PBS containing 0.02% sodium azide.

SIRP alpha Antibody: Protein tyrosine phosphatases (PTPases) SHP-1 and SHP-2 are critical regulators in the intracellular signaling pathways that result in cell responses such as mitosis, differentiation, migration, survival, transformation or death. SHP-2 is a signal transducer for several receptor tyrosine kinases and cytokine receptors. A novel SHP-2 associated glycoprotein was recently cloned from human, rat, mouse and cattle by several labs and was designated SIRPalpha, SHPS-1, MyD-1, BIT and p84. SIRPalpha is a new gene family containing at least fifteen members. SIRPalpha is a substrate of many activated tyrosine kinases such as insulin receptor, EGFR, PDGFR and src, and a specific docking protein for SHP-2. SIRPalpha has regulatory effects on cellular responses induced by serum, growth factors, insulin, oncogenes, growth hormones and cell adhesion and plays a general role in different physiological and pathological processes.

18426911

NP_542970

NM_080792.2

P78324

75 - 110 kDa

BDNF Signaling Pathway (712093); Cardiac Progenitor Differentiation Pathway (712094); Cell Surface Interactions At The Vascular Wall Pathway (106062); Cell-Cell Communication Pathway (477132); Hemostasis Pathway (106028); IL-1 Signaling Pathway (198908); Osteoclast Differentiation Pathway (193147); Osteoclast Differentiation Pathway (193096); Prolactin Signaling Pathway (672462); Signal Regulatory Protein (SIRP) Family Interactions Pathway (187209)

The protein encoded by this gene is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. This protein can be phosphorylated by tyrosine kinases. The phospho-tyrosine residues of this PTP have been shown to recruit SH2 domain containing tyrosine phosphatases (PTP), and serve as substrates of PTPs. This protein was found to participate in signal transduction mediated by various growth factor receptors. CD47 has been demonstrated to be a ligand for this receptor protein. This gene and its product share very high similarity with several other members of the SIRP family. These related genes are located in close proximity to each other on chromosome 20p13. Multiple alternatively spliced transcript variants have been determined for this gene. [provided by RefSeq, Jul 2008]

SHPS1: Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function. Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Binds PTPN11 when tyrosine-phosphorylated, except in macrophages, where it primarily binds PTPN6. Binds GRB2 in vitro. Binds FGR. Binds JAK2 irrespective of its phosphorylation status and forms a stable complex. Binds SCAP1 and/or SCAP2. The resulting complex recruits FYB. Binds PTK2B. Ubiquitous. Highly expressed in brain. Detected on myeloid cells, but not T-cells. Detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis; Receptor, misc.; Cell surface. Chromosomal Location of Human Ortholog: 20p13. Cellular Component: membrane; integral to membrane; plasma membrane. Molecular Function: SH3 domain binding. Biological Process: cell adhesion; blood coagulation; leukocyte migration

0.1 mg

345 USD