BMI-1 Antibody | MyBioSource MBS150191 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

BMI-1 Antibody

catalog :

MBS150191

quantity :

0.1 mg

price :

345 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunocytochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS150191

products type :

Antibody

products full name :

BMI-1 Antibody

products short name :

BMI-1

products name syn :

BMI-1; PCGF4; RNF51; FLVI2/BMI1; PCGF4; Polycomb complex protein BMI-1; Polycomb group RING finger protein 4; BMI1 polycomb ring finger oncogene

other names :

Polycomb complex protein BMI-1; Polycomb complex protein BMI-1; COMMD3-BMI1 read-through protein; COMMD3-BMI1 readthrough; Polycomb group RING finger protein 4; RING finger protein 51

products gene name :

BMI1

other gene names :

COMMD3-BMI1; BMI1; PCGF4; RNF51

uniprot entry name :

BMI1_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

326

purity :

BMI-1 Antibody is affinity chromatography purified via peptide column.

form :

Liquid

concentration :

1 mg/mL

storage stability :

BMI-1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

tested application :

ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

app notes :

BMI-1 antibody can be used for detection of BMI-1 by Western blot at 0.5 to 2 mug/mL. Antibody can also be used for immunocytochemistry starting at 10 mug/mL. For immunofluorescence start at 20 mug/mL.

other info1 :

Conjugate: Unconjugated. Immunogen: BMI-1 antibody was raised against a peptide corresponding to 15 amino acids near the center of human BMI-1. Buffer: BMI-1 Antibody is supplied in PBS containing 0.02% sodium azide.

products description :

BMI-1 Antibody: The transcriptional repressor BMI-1 was first identified as a proto-oncogene frequently activated by Moloney murine leukemia proviral insertions in mice and cooperating with c-myc in the generation of mouse lymphomas. BMI-1 is involved in segment specification, cell growth and maintenance, transcriptional regulation, and chromatin modification. A major target of BMI-1 is the ink4a locus which encodes tumor suppressor proteins p16 and p19Arf, which are important in tumor progression and thought to be critical in cell proliferation and senescence. Recent studies have also shown that BMI-1 is required for the maintenance of adult normal and leukemic stem cells, suggesting that BMI-1 could an attractive therapeutic target for stem cell proliferation and renewal as well as for anti-cancer strategies.

ncbi gi num :

22258801

ncbi acc num :

P35226.2

uniprot acc num :

P35226

ncbi mol weight :

36,949 Da

ncbi pathways :

MicroRNAs In Cancer Pathway (852705); MicroRNAs In Cancer Pathway (852928); Senescence And Autophagy Pathway (198780); Signaling Pathways Regulating Pluripotency Of Stem Cells (1026136); Signaling Pathways Regulating Pluripotency Of Stem Cells (1033502); Transcriptional Misregulation In Cancer Pathway (523016); Transcriptional Misregulation In Cancer Pathway (522987); Validated Targets Of C-MYC Transcriptional Activation Pathway (169351)

ncbi summary :

This locus represents naturally occurring read-through transcription between the neighboring COMM domain-containing protein 3 and polycomb complex protein BMI-1 genes on chromosome 10. The read-through transcript produces a fusion protein that shares sequence identity with each individual gene product. [provided by RefSeq, Feb 2011]

uniprot summary :

BMI1: Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A Lys-119 , rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2. Component of a PRC1-like complex. Interacts with RING1 and RING2. Interacts vwith CBX7 and CBX8. Interacts with SPOP. Part of a complex consisting of BMI1, CUL3 and SPOP. Interacts with E4F1. Protein type: Ubiquitin ligase; Ubiquitin conjugating system; Ligase; EC 6.3.2.-; Oncoprotein; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 10p11.23. Cellular Component: nucleoplasm; heterochromatin; nuclear body; cytoplasm; PcG protein complex; nucleus; ubiquitin ligase complex. Molecular Function: protein binding; zinc ion binding; sequence-specific DNA binding; ubiquitin-protein ligase activity; chromatin binding. Biological Process: transcription, DNA-dependent; in utero embryonic development; positive regulation of immature T cell proliferation in the thymus; negative regulation of transcription from RNA polymerase II promoter; humoral immune response; embryonic skeletal morphogenesis; segment specification; rostrocaudal neural tube patterning; positive regulation of fibroblast proliferation; regulation of gene expression; positive regulation of ubiquitin-protein ligase activity; histone ubiquitination; positive regulation of B cell proliferation; DNA methylation; somatic stem cell division; hemopoiesis; histone acetylation; brain development

size1 :

0.1 mg

price1 :

345 USD

more info or order :

MyBioSource product webpage