antibody

Bfl-1 Antibody

MBS150190

0.1 mg

345 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay

Antibody

Bfl-1 Antibody

Bfl-1

Bfl-1; GRS; BFL1; ACC-1; ACC-2; HBPA1; BCL2L5; GRS; Bcl-2-related protein A1; Bcl-2-like protein 5; Bcl2-L-5; BCL2-related protein A1

bcl-2-related protein A1 isoform 1; Bcl-2-related protein A1; bcl-2-related protein A1; bcl2-L-5; protein BFL-1; bcl-2-like protein 5; hematopoietic BCL2-related protein A1; hemopoietic-specific early response protein; BCL2-related protein A1; Bcl-2-like protein 5; Bcl2-L-5; Hemopoietic-specific early response protein; Protein BFL-1; Protein GRS

BCL2A1

BCL2A1; BCL2A1; GRS; BFL1; ACC-1; ACC-2; HBPA1; BCL2L5; BCL2L5; BFL1; GRS; HBPA1; Bcl2-L-5

B2LA1_HUMAN

Polyclonal

IgG

Rabbit

Human, Mouse, Rat

175

Bfl-1 Antibody is affinity chromatography purified via peptide column.

Liquid

1 mg/mL

Bfl-1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Bfl-1 antibody can be used for the detection of Bfl-1 by Western blot at 1 - 2 mug/mL. Presumably due to post-translational modifications, Bfl-1 is often observed at higher molecular weight in SDS-PAGE than its predicted molecular weight would suggest. For immunofluorescence start at 20 mug/mL.

Conjugate: Unconjugated. Immunogen: Bfl-1 antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus of human Bfl-1. Buffer: Bfl-1 Antibody is supplied in PBS containing 0.02% sodium azide.

Bfl-1 Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells and is caused by caspase activation. Proteins that comprise the Bcl-2 family appear to control the activation of these enzymes. One such member is multi-domain antiapoptotic protein Bfl-1, which is overexpressed in stomach and other cancers. Bfl-1 can interact with Bax and suppress apoptosis by inhibiting the release of cytochrome c and caspase-3 activation. It is upregulated in cisplatin-resistant human bladder tumors, suggesting that its expression may be important for cisplatin resistance and inhibition of apoptosis in cancer cells. At least two isoforms of Bfl-1 are known to exist.

4757840

NP_004040

NM_004049.3

Q16548

18,746 Da

Apoptosis Modulation And Signaling Pathway (198822); BCR Signaling Pathway (138058); Direct P53 Effectors Pathway (137939); NF-kappa B Signaling Pathway (634527); Transcriptional Misregulation In Cancer Pathway (523016); Transcriptional Misregulation In Cancer Pathway (522987)

This gene encodes a member of the BCL-2 protein family. The proteins of this family form hetero- or homodimers and act as anti- and pro-apoptotic regulators that are involved in a wide variety of cellular activities such as embryonic development, homeostasis and tumorigenesis. The protein encoded by this gene is able to reduce the release of pro-apoptotic cytochrome c from mitochondria and block caspase activation. This gene is a direct transcription target of NF-kappa B in response to inflammatory mediators, and is up-regulated by different extracellular signals, such as granulocyte-macrophage colony-stimulating factor (GM-CSF), CD40, phorbol ester and inflammatory cytokine TNF and IL-1, which suggests a cytoprotective function that is essential for lymphocyte activation as well as cell survival. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

BCL2A1: Retards apoptosis induced by IL-3 deprivation. May function in the response of hemopoietic cells to external signals and in maintaining endothelial survival during infection. Interacts directly with BAK1, BID, BMF and BBC3. Interacts directly with BCL2L11/BIM. Interacts with BAX isoform Sigma. Interacts directly with PMAIP1. By phorbol ester and inflammatory cytokines, such as TNF or IL1B/interleukin-1 beta, but not by growth factors. Seems to be restricted to the hematopoietic compartment. Expressed in peripheral blood, spleen, and bone marrow, at moderate levels in lung, small intestine and testis, at a minimal levels in other tissues. Also found in vascular smooth muscle cells and hematopoietic malignancies. Belongs to the Bcl-2 family. 2 isoforms of the human protein are produced by alternative splicing. Chromosomal Location of Human Ortholog: 15q24.3. Cellular Component: mitochondrial outer membrane. Molecular Function: BH domain binding; protein binding; protein homodimerization activity; protein heterodimerization activity. Biological Process: DNA damage response, signal transduction resulting in induction of apoptosis; negative regulation of apoptosis

0.1 mg

345 USD