antibody

UCMA Antibody

MBS150184

0.1 mg

375 USD

polyclonal

rabbit

nonconjugated

human, mouse

western blot, ELISA

Antibody

UCMA Antibody

[UCMA]

[UCMA Antibody: GRP, C10orf49, Unique cartilage matrix-associated protein, Gla-rich protein, Ucma-C]

[unique cartilage matrix-associated protein isoform 1 preproprotein; Unique cartilage matrix-associated protein; unique cartilage matrix-associated protein; Gla-rich protein; upper zone of growth plate and cartilage matrix associated; Gla-rich protein; GRP]

[UCMA]

[UCMA; UCMA; GRP; C10orf49; GRP/UCMA; C10orf49; Ucma-C; GRP]

UCMA_HUMAN

Polyclonal

IgG

Rabbit

Human, Mouse

UCMA Antibody is affinity chromatography purified via peptide column.

Liquid; UCMA Antibody is supplied in PBS containing 0.02% sodium azide.

1 mg/mL

UCMA antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

ELISA, Western Blot (WB)

UCMA antibody can be used for detection of UCMA by Western blot at 2.5 - 5 ug/mL. Antibody validated: Western Blot in human samples. All other applications and species not yet tested. Optimal dilutions for each application to be determined by the researcher.

Western Blot (WB)

Homology: Predicted species reactivity based on immunogen sequence: Rat: (81%)

Immunogen: UCMA antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human UCMA. The immunogen is located within the last 50 amino acids of UCMA. Positive Control: 1) Cat. No. MBS151599 - SW1353 Cell Lysate. Conjugate: Unconjugated

UCMA Antibody: UCMA is a secreted cartilage-specific protein that was discovered in a screen for differentially expressed genes in retinoic acid-treated mouse chondrocytes. It was also identified in a human chondrocyte EST screen for candidate genes of skeletal dysplasias. UCMA expression is thought to parallel that of collagen II with its expression decreasing with maturation chrondrocytes mature. UCMA is processed by a furin-like protease into two fragments, an amino-terminal fragment and a carboxy-terminal fragment (UCMA-C). Application of recombinant UCMA-C to primary osteoblasts, mesenchymal stem cells, and MC3T3-E1 pre-osteoblasts interferes with their osteogenic differentiation, but does not affect expression of chondrocyte-specific genes or chondrocyte proliferation, suggesting that UCMA may be involved in the negative control of osteogenic differentiation of osteochondrogenic precursor cells. At least two isoforms of UCMA are known to exist.

50400088

NP_660357

NM_145314.2

Q8WVF2

Predicted: 15 kDa. Observed: 19 kDa

This gene encodes a chondrocyte-specific, highly charged protein that is abundantly expressed in the upper immature zone of fetal and juvenile epiphyseal cartilage. The encoded protein undergoes proteolytic processing to generate a mature protein that is secreted into the extracellular matrix. The glutamic acid residues in the encoded protein undergo gamma carboxylation in a vitamin K-dependent manner. Undercarboxylation of the encoded protein is associated with osteoarthritis in humans. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2015]

UCMA: May be involved in the negative control of osteogenic differentiation of osteochondrogenic precursor cells in peripheral zones of fetal cartilage and at the cartilage-bone interface. Belongs to the UCMA family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 10p13. Cellular Component: proteinaceous extracellular matrix; extracellular space; perinuclear region of cytoplasm. Biological Process: negative regulation of osteoblast differentiation

0.1 mg

375 USD