antibody

XAF-1 Antibody

MBS150176

0.1 mg

345 USD

polyclonal

rabbit

nonconjugated

human, mouse

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay

Antibody

XAF-1 Antibody

XAF-1

XAF-1; BIRC4BP; XIAPAF1; HSXIAPAF1; BIRC4BP; XIAP-associated factor 1; BIRC4-binding protein; XIAP associated factor 1

XIAP associated factor-1 (ZAP-1); XIAP-associated factor 1; XIAP-associated factor 1; BIRC4 binding protein; BIRC4-binding protein; XIAP associated factor 1; BIRC4-binding protein

XAF1

XAF1; XAF1; BIRC4BP; XIAPAF1; HSXIAPAF1; BIRC4BP; XIAPAF1

XAF1_HUMAN

Polyclonal

IgG

Rabbit

Human, Mouse

317

XAF-1 Antibody is affinity chromatography purified via peptide column.

Liquid

1 mg/mL

XAF-1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

XAF-1 antibody can be used for the detection of XAF-1 by Western blot at 0.5 to 2 mug/mL. A 32 kDa band can be detected. Antibody can also be used for immunohistochemistry starting at 2 mug/mL. For immunofluorescence start at 10 mug/mL.

Conjugate: Unconjugated. Immunogen: XAF-1 antibody was raised against a synthetic peptide corresponding to amino acids at the C-terminus of human XAF-1. Buffer: XAF-1 Antibody is supplied in PBS containing 0.02% sodium azide.

XAF-1 Antibody: XAF-1 binds to XIAP, an inhibitor of caspases-3, -7, and -9, and triggers its relocation from the cytosol to the nucleus. Overexpression of XAF-1 results in the neutralization of XIAP's ability to inhibit cell death. XAF-1 is normally expressed in all adult and fetal tissues but was found to be present in very low levels in a variety of cancer cell lines. In contrast, XIAP levels have been shown to be high in a majority of cell lines. Low XAF-1 and high basal expression of XIAP may therefore play a critical role in maintaining survival of cancer cell lines. Both IFN-alpha2 and IFN-beta can induce XAF-1 mRNA in all cells examined but induction of XAF-1 protein (as observed by immunoblot analysis) was seen only in cell lines sensitive to the apoptotic effects of IFNs.

1869901

CAA68030

Q6GPH4

34,543 Da

Cytokine Signaling In Immune System Pathway 366171!!Immune System Pathway 106386!!Interferon Signaling Pathway 187103!!Interferon Alpha/beta Signaling Pathway 187104

This gene encodes a protein which binds to and counteracts the inhibitory effect of a member of the IAP (inhibitor of apoptosis) protein family. IAP proteins bind to and inhibit caspases which are activated during apoptosis. The proportion of IAPs and proteins which interfere with their activity, such as the encoded protein, affect the progress of the apoptosis signaling pathway. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]

0.1 mg

345 USD