antibody

Apaf1 Antibody

MBS150175

0.1 mg

345 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay

Antibody

Apaf1 Antibody

Apaf1

Apaf1; CED4; APAF-1; KIAA0413; Apoptotic protease-activating factor 1; apoptotic peptidase activating factor 1

apoptotic protease activating factor 1; Apoptotic protease-activating factor 1; apoptotic protease-activating factor 1; apoptotic peptidase activating factor 1

APAF1

APAF1; APAF1; CED4; APAF-1; KIAA0413; APAF-1

APAF_HUMAN

Polyclonal

IgG

Rabbit

Human, Mouse, Rat

1194

Apaf1 Antibody is affinity chromatography purified via peptide column.

Liquid

Apaf1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Apaf1 antibody can be used for detection of Apaf1 by Western blot at 1 mug/mL. A 115 - 130 kDa band should be detected. Antibody can also be used for immunohistochemistry starting at 1 mug/mL. For immunofluorescence start at 10 mug/mL.

Conjugate: Unconjugated. Immunogen: Apaf1 antibody was raised against a peptide corresponding to amino acids near the amino terminus of human Apaf1. The sequences of the immunogenic peptide are identical between human and mouse. Buffer: Apaf1 Antibody is supplied in PBS containing 0.02% sodium azide.

Apaf1 Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf1 (for apoptosis protease-activating factor 1). Apaf1 binds to cytochrome c (Apaf-2) and caspase-9 (Apaf-3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf1 can also associate with caspase-4 and caspase-8. Apaf1 is ubiquitously expressed in human tissues.

2330015

AAC51678

O14727

115 - 130 kDa

Activation Of Caspases Through Apoptosome-mediated Cleavage Pathway 105672!!Alzheimer's Disease Pathway 83097!!Alzheimer's Disease Pathway 509!!Alzheimers Disease Pathway 672448!!Amyotrophic Lateral Sclerosis (ALS) Pathway 920975!!Amyotrophic Lateral Sclerosis (ALS) Pathway 83099!!Amyotrophic Lateral Sclerosis (ALS) Pathway 511!!Apoptosis Pathway 198797!!Apoptosis Pathway 83060!!Apoptosis Pathway 470

This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

0.1 mg

345 USD