antibody

Nudel Antibody

MBS150108

0.1 mg

345 USD

polyclonal

rabbit

nonconjugated

human, mouse

western blot, ELISA, immunocytochemistry, enzyme immunoassay

Antibody

Nudel Antibody

Nudel

Nudel; EOPA; NDE2; NUDEL; MITAP1; NDE1L1; EOPA; Nuclear distribution protein nudE-like 1; Mitosin-associated protein 1; Protein Nudel; nudE nuclear distribution gene E homolog (A. nidulans)-like 1

Nudel; Nuclear distribution protein nudE-like 1; nuclear distribution protein nudE-like 1; protein Nudel; endooligopeptidase A; mitosin-associated protein 1; mitosin-associated protein MITAP1; nudE nuclear distribution E homolog-like 1; nudE nuclear distribution gene E homolog-like 1; nudE neurodevelopment protein 1-like 1; Mitosin-associated protein 1

NDEL1

NDEL1; NDEL1; EOPA; NDE2; NUDEL; MITAP1; NDE1L1; EOPA; MITAP1; NUDEL; Protein Nudel

NDEL1_HUMAN

Polyclonal

IgG

Rabbit

Human, Mouse

345

Nudel Antibody is affinity chromatography purified via peptide column.

Liquid

1 mg/mL

Nudel antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Nudel antibody can be used for detection of Nudel by Western blot at 0.5 - 2 mug/mL. Antibody can also be used for immunocytochemistry starting at 2 mug/mL. For immunofluorescence start at 10 mug/mL.

Conjugate: Unconjugated. Immunogen: Nudel antibody was raised against a peptide corresponding to 15 amino acids near the amino terminus of human Nudel. Buffer: Nudel Antibody is supplied in PBS containing 0.02% sodium azide.

Nudel Antibody: Nudel was initially discovered as a protein homologous to Aspergillus NUDE and that associated with Lis1 and polyprotein complex cytoplasmic dynein, both of which have been linked to neuronal development and migration. It was later shown to be a substrate of cdk5, a kinase known to be critical during neuronal migration; phosphorylation of Nudel by cdk5 affects its localization in neurons and affects neuritic morphology. It is thought that together with Lis1, Nudel regulates cytoplasmic dynein, a large polyprotein complex, in neuronal migration and mitosis through direct interactions. Similar interactions are thought to also play a role in membrane traffic in other cells as disruption of Nudel expression through RNA interference perturbed normal endomembrane flux and resulted in the fragmentation of the Golgi apparatus.

12043569

AAF97497

Q9GZM8

37,039 Da

Aurora A Signaling Pathway (137925); Cell Cycle Pathway (530733); Cell Cycle, Mitotic Pathway (105765); Lissencephaly Gene (LIS1) In Neuronal Migration And Development Pathway (137984); M Phase Pathway (105812); Mitotic Anaphase Pathway (105818); Mitotic Metaphase And Anaphase Pathway (730317); Mitotic Prometaphase Pathway (105815); Resolution Of Sister Chromatid Cohesion Pathway (730315); Separation Of Sister Chromatids Pathway (730318)

This gene encodes a coiled-coil protein that plays a role in multiple processes including cytoskeletal organization, cell signaling and neuron migration, outgrowth and maintenance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome X. [provided by RefSeq, Mar 2012]

NDEL1: Required for organization of the cellular microtubule array and microtubule anchoring at the centrosome. May regulate microtubule organization at least in part by targeting the microtubule severing protein KATNA1 to the centrosome. Also positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus ends. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the centripetal motion of secretory vesicles and the coupling of the nucleus and centrosome. Also required during brain development for the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Plays a role, together with DISC1, in the regulation of neurite outgrowth. Required for mitosis in some cell types but appears to be dispensible for mitosis in cortical neuronal progenitors, which instead requires NDE1. Facilitates the polymerization of neurofilaments from the individual subunits NEFH and NEFL. Belongs to the nudE family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Protease; Microtubule-binding. Chromosomal Location of Human Ortholog: 17p13.1. Cellular Component: axon hillock; kinetochore; microtubule; kinesin complex; centrosome; neurofilament cytoskeleton; cytoplasm; leading edge; nucleolus; spindle; nuclear envelope; cytosol. Molecular Function: protein binding; microtubule binding; beta-tubulin binding; alpha-tubulin binding. Biological Process: positive regulation of axon regeneration; cell migration; central nervous system neuron axonogenesis; positive regulation of axon extension; neuron migration; establishment of chromosome localization; nuclear envelope disassembly; proteolysis; microtubule nucleation; retrograde axon cargo transport; inner cell mass cell proliferation; chromosome segregation; mitotic centrosome separation; neurofilament cytoskeleton organization and biogenesis; establishment of mitotic spindle orientation; centrosome localization; vesicle transport along microtubule; cerebral cortex radially oriented cell migration; mitotic cell cycle

0.1 mg

345 USD