antibody

TACE Antibody

MBS150091

0.1 mg

345 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, ELISA, immunocytochemistry, flow cytometry, FACS, enzyme immunoassay

Antibody

TACE Antibody

TACE

TACE; CSVP; TACE; NISBD; ADAM18; CD156B; CSVP; Disintegrin and metalloproteinase domain-containing protein 17; Snake venom-like protease; ADAM 17; ADAM metallopeptidase domain 17

disintegrin and metalloproteinase domain-containing protein 17 preproprotein; Disintegrin and metalloproteinase domain-containing protein 17; disintegrin and metalloproteinase domain-containing protein 17; TNF-alpha convertase; snake venom-like protease; TNF-alpha converting enzyme; ADAM metallopeptidase domain 18; tumor necrosis factor, alpha, converting enzyme; ADAM metallopeptidase domain 17; Snake venom-like protease; TNF-alpha convertase; TNF-alpha-converting enzyme; CD_antigen: CD156b

ADAM17

ADAM17; ADAM17; CSVP; TACE; NISBD; ADAM18; CD156B; NISBD1; CSVP; TACE; ADAM 17

ADA17_HUMAN

Polyclonal

IgG

Rabbit

Human, Mouse, Rat

824

80 to 130 kDa bands can be detected, which may represent mature protein, precursor, and glycosylated TACE.

TACE Antibody is affinity chromatography purified via peptide column.

Liquid

1 mg/mL

TACE antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)

TACE antibody can be used for detection of TACE by Western blot at 0.5 mug/mL. For immunocytochemistry use 10 mug/mL. For immunofluorescence start at 10 mug/mL.

Conjugate: Unconjugated. Immunogen: TACE antibody was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human TACE. Buffer: TACE Antibody is supplied in PBS containing 0.02% sodium azide.

TACE Antibody: Tumor-necrosis factor-alpha is a proinflammatory cytokine and contributes to a variety of inflammatory disease responses and programmed cell death. TNF- is synthesized as a 26K type II membrane-bound precursor that is cleaved by a convertase to generate secreted 17K mature TNF- . TNF- converting enzyme (TACE) protein was recently purified and the human and mouse TACE cDNAs were cloned by several groups separately. TACE is a membrane-bound metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease). TACE also processes other cell surface proteins, including TNF receptor, TGFalpha, the L-selectin adhesion molecule, and alpha-cleavage of amyloid protein precursor (APP). TACE mRNA is expressed in a variety of human and murine tissues. TACE was selected as one of the few targets in cytokine activation by the Eighth International Conference of the Inflammation Research Association.

73747889

NP_003174

NM_003183.4

P78536

Predicted: 93 kDa

Activated NOTCH1 Transmits Signal To The Nucleus Pathway (576270); Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Alzheimers Disease Pathway (672448); BDNF Signaling Pathway (712093); Collagen Degradation Pathway (730309); Constitutive Signaling By NOTCH1 HD Domain Mutants Pathway (771591); Constitutive Signaling By NOTCH1 HD+PEST Domain Mutants Pathway (771595); Constitutive Signaling By NOTCH1 PEST Domain Mutants Pathway (771593); Constitutive Signaling By NOTCH1 T(7;9)(NOTCH1:M1580_K2555) Translocation Mutant Pathway (771589)

This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene functions as a tumor necrosis factor-alpha converting enzyme; binds mitotic arrest deficient 2 protein; and also plays a prominent role in the activation of the Notch signaling pathway. [provided by RefSeq, Jul 2008]

TACE: a type I membrane protein with disintegrin and metalloprotease activity. An ubiquitously expressed ectoenzyme of peptidase family M12B. Must be membrane anchored to cleave the different substrates. The cytoplasmic domain is not required for the this activity. Possesses a narrow endopeptidase specificity. Cleaves Pro-Leu-Ala-Gln-Ala- -Val-Arg-Ser-Ser-Ser in the membrane-bound, 26-kDa form of tumor necrosis factor alpha (TNF-alpha) to its mature soluble form. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, and the amyloid precursor protein. Also involved in the activation of Notch pathway. Binds 1 zinc ion per subunit. Two splice variant isoforms have been described. Protein type: Protease; Membrane protein, integral; Motility/polarity/chemotaxis; EC 3.4.24.86. Chromosomal Location of Human Ortholog: 2p25. Cellular Component: focal adhesion; cell surface; membrane; integral to plasma membrane; apical plasma membrane; cytoplasm; plasma membrane; intercellular junction; actin cytoskeleton; lipid raft. Molecular Function: integrin binding; protein binding; interleukin-6 receptor binding; metallopeptidase activity; zinc ion binding; metalloendopeptidase activity; Notch binding; SH3 domain binding; PDZ domain binding. Biological Process: extracellular matrix organization and biogenesis; nerve growth factor receptor signaling pathway; T cell differentiation in the thymus; neutrophil mediated immunity; cell motility involved in cell locomotion; positive regulation of leukocyte chemotaxis; response to lipopolysaccharide; proteolysis; G1/S-specific positive regulation of cyclin-dependent protein kinase activity; extracellular matrix disassembly; positive regulation of epidermal growth factor receptor activity; positive regulation of cell proliferation; negative regulation of interleukin-8 production; germinal center formation; cell adhesion; epidermal growth factor receptor signaling pathway; response to drug; spleen development; Notch signaling pathway; response to high density lipoprotein stimulus; membrane protein intracellular domain proteolysis; membrane protein ectodomain proteolysis; positive regulation of transforming growth factor beta receptor signaling pathway; Notch receptor processing; positive regulation of cell motility; positive regulation of cell growth; positive regulation of chemokine production; regulation of mast cell apoptosis; collagen catabolic process; PMA-inducible membrane protein ectodomain proteolysis; B cell differentiation; response to hypoxia; cell adhesion mediated by integrin; positive regulation of protein amino acid phosphorylation; negative regulation of transforming growth factor beta receptor signaling pathway; wound healing, spreading of epidermal cells; positive regulation of cell migration. Disease: Inflammatory Skin And Bowel Disease, Neonatal, 1

0.1 mg

345 USD