antibody

RIPK1 Antibody

MBS150077

0.1 mg

345 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay

Antibody

RIPK1 Antibody

RIPK1

RIPK1; RIP; RIP1; RIP; Cell death protein RIP; RIP-1; receptor (TNFRSF)-interacting serine-threonine kinase 1

receptor-interacting serine/threonine-protein kinase 1; Receptor-interacting serine/threonine-protein kinase 1; receptor-interacting serine/threonine-protein kinase 1; RIP-1; cell death protein RIP; receptor interacting protein; receptor-interacting protein 1; serine/threonine-protein kinase RIP; receptor (TNFRSF)-interacting serine-threonine kinase 1; Cell death protein RIP; Receptor-interacting protein 1; RIP-1; Serine/threonine-protein kinase RIP

RIPK1

RIPK1; RIPK1; RIP; RIP1; RIP; RIP1; RIP-1

RIPK1_HUMAN

Polyclonal

IgG

Rabbit

Human, Mouse, Rat

671

RIPK1 Antibody is affinity chromatography purified via peptide column.

Liquid

1 mg/mL

RIPK1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

RIPK1 antibody can be used for detection of RIPK1 by Western blot at 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.

Conjugate: Unconjugated. Immunogen: RIPK1 antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human RIPK1. Buffer: RIPK1 Antibody is supplied in PBS containing 0.02% sodium azide.

RIPK1 Antibody: RIPK1 (Receptor Interacting Protein) is a crucial 74 kD adaptor kinase in several of stress-induced signaling pathways and on the crossroad of a cell's decision to live or die. RIPK1 contains an N-terminal region with homology to protein kinases, an intermediate domain capable of association with MAPKKK and a C-terminal region containing a death domain motif present in the Fas and TNFR1 intracellular domains. Full length RIPK1 is important for signallling to NF -B, MAPKs and necrosis, whereas caspase-8 generates a C-terminal RIPK1 cleavage fragment, promoting TNF-induced apoptosis. It is required for TNFRSF1A-mediated and TLR3-induced NF- B activation. RIPK1-deficient mice fail to thrive, displaying extensive apoptosis in both lymphoid and adipose tissues and dying at 1-3 days of age.

57242761

NP_003795

NM_003804.3

Q13546

70,733 Da

Activated TLR4 Signalling Pathway (106400); Apoptosis Pathway (198797); Apoptosis Pathway (83060); Apoptosis Pathway (470); Apoptosis Pathway (105648); Apoptosis Modulation By HSP70 Pathway (198877); Caspase Cascade In Apoptosis Pathway (137974); Caspase-8 Activation By Cleavage Pathway (105654); Ceramide Signaling Pathway (138023); Cytosolic DNA-sensing Pathway (125137)

RIPK1: Serine-threonine kinase which transduces inflammatory and cell-death signals (necroptosis) following death receptors ligation, activation of pathogen recognition receptors (PRRs), and DNA damage. Upon activation of TNFR1 by the TNF-alpha family cytokines, TRADD and TRAF2 are recruited to the receptor. Ubiquitination by TRAF2 via Lys-63 -link chains acts as a critical enhancer of communication with downstream signal transducers in the mitogen-activated protein kinase pathway and the NF-kappa-B pathway, which in turn mediate downstream events including the activation of genes encoding inflammatory molecules. Polyubiquitinated protein binds to IKBKG/NEMO, the regulatory subunit of the IKK complex, a critical event for NF-kappa-B activation. Interaction with other cellular RHIM-containing adapters initiates gene activation and cell death. RIPK1 and RIPK3 association, in particular, forms a necroptosis-inducing complex. Interacts (via RIP homotypic interaction motif) with RIPK3 (via RIP homotypic interaction motif); this interaction induces RIPK1 necroptosis-specific phosphorylation, formation of the necroptosis-inducing complex. Interacts (via the death domain) with TNFRSF6 (via the death domain) and TRADD (via the death domain). Is recruited by TRADD to TNFRSF1A in a TNF-dependent process. Binds RNF216, EGFR, IKBKG, TRAF1, TRAF2 and TRAF3. Interacts with BNLF1. Interacts with SQSTM1 upon TNF-alpha stimulation. May interact with MAVS/IPS1. Interacts with ZFAND5. Interacts with RBCK1. Interacts with BIRC2/c-IAP1, BIRC3/c-IAP2 and XIAP/BIRC4. Inhibited by necrostatin-1. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; Protein kinase, TKL; TKL group; RIPK family. Chromosomal Location of Human Ortholog: 6p25.2. Cellular Component: mitochondrion; cytoplasm; cytosol; receptor complex; lipid raft. Molecular Function: identical protein binding; protein serine/threonine kinase activity; protein binding; ubiquitin protein ligase binding; death receptor binding; protein complex binding; ATP binding; protein kinase activity. Biological Process: caspase activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; protein heterooligomerization; positive regulation of apoptosis; apoptosis; MyD88-independent toll-like receptor signaling pathway; protein amino acid autophosphorylation; positive regulation of JNK cascade; negative regulation of I-kappaB kinase/NF-kappaB cascade; toll-like receptor 3 signaling pathway; positive regulation of tumor necrosis factor production; activation of JNK activity; activation of NF-kappaB transcription factor; positive regulation of interleukin-8 production; positive regulation of programmed cell death; tumor necrosis factor-mediated signaling pathway; cellular protein catabolic process; toll-like receptor signaling pathway; positive regulation of interferon type I production; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; positive regulation of macrophage differentiation; toll-like receptor 4 signaling pathway; protein homooligomerization

0.1 mg

345 USD