TRIM5 gamma Antibody | MyBioSource MBS150065 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

TRIM5 gamma Antibody

catalog :

MBS150065

quantity :

0.1 mg

price :

345 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS150065

products type :

Antibody

products full name :

TRIM5 gamma Antibody

products short name :

TRIM5 gamma

products name syn :

TRIM5 gamma; RNF88; TRIM5alpha; RNF88; Tripartite motif-containing protein 5; RING finger protein 88; tripartite motif-containing 5

other names :

tripartite motif protein TRIM5 isoform gamma; Tripartite motif-containing protein 5; tripartite motif-containing protein 5; ring finger protein 88; tripartite motif protein TRIM5; tripartite motif containing 5 transcript variant iota; tripartite motif containing 5 transcript variant kappa; tripartite motif containing 5; RING finger protein 88

products gene name :

TRIM5

other gene names :

TRIM5; TRIM5; RNF88; TRIM5alpha; RNF88

uniprot entry name :

TRIM5_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human

sequence length :

347

purity :

TRIM5 gamma Antibody is affinity chromatography purified via peptide column.

form :

Liquid

concentration :

1 mg/mL

storage stability :

TRIM5 gamma antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

tested application :

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

app notes :

TRIM5 gamma antibody can be used for detection of TRIM5 gamma by Western blot at 2 mug/mL. A band at approximately 35 kDa can be detected. Antibody can also be used for immunohistochemistry starting at 2 mug/mL. For immunofluorescence start at 20 mug/mL.

other info1 :

Conjugate: Unconjugated. Immunogen: TRIM5 gamma antibody was raised against a synthetic peptide corresponding to 15 amino acids near the carboxy terminus of human TRIM5 gamma. Buffer: TRIM5 gamma Antibody is supplied in PBS containing 0.02% sodium azide.

products description :

TRIM5 gamma Antibody: TRIM5 is a member of a broad family of otherwise unrelated proteins defined by the presence of a tripartite motif containing a RING domain, a B-box type 1, and a B-box type 2, followed by a coiled-coil region. TRIM5 has five alternately spliced isoforms, the longest of which is the alpha variant which also contains a carboxy-terminal B30.2 (SPRY) domain. While a function has not yet been assigned to TRIM5gamma, it is known that expression of TRIM5 variants from humans, rhesus monkeys, and African green monkeys enable resistance to infection by various retroviruses including HIV-1, albeit at differing efficiencies. Furthermore, the TRIM5 isoform appears to serve as a scaffold for the assembly of endogenous BTBD1 and BTBD2 proteins and also exhibits autoubiquitination activity in a RING finger- and UbcH5B-dependent manner.

ncbi gi num :

15011944

ncbi acc num :

NP_149083

ncbi gb acc num :

NM_033092.1

uniprot acc num :

Q9C035

ncbi mol weight :

29,677 Da

ncbi pathways :

Cytokine Signaling In Immune System Pathway (366171); Immune System Pathway (106386); Interferon Signaling Pathway (187103); Interferon Gamma Signaling Pathway (187106)

ncbi summary :

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein forms homo-oligomers via the coilel-coil region and localizes to cytoplasmic bodies. It appears to function as a E3 ubiquitin-ligase and ubiqutinates itself to regulate its subcellular localization. It may play a role in retroviral restriction. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Dec 2009]

uniprot summary :

TRIM5: Isoform Alpha is a retrovirus restriction factor, which mediates species-specific, early block to retrovirus infection. Targets retroviral capsid soon after entry into the cell, and prevents reverse transcription of the virus RNA genome. Isoform Alpha trimers may make multiple contacts with the hexameric lattice of CA proteins which constitute the surface of retrovirion core, and somehow inactivate the virus. Restricts efficiently infection by N-MLV, but not HIV-1. May have E3 ubiquitin-protein ligase activity. Belongs to the TRIM/RBCC family. 6 isoforms of the human protein are produced by alternative splicing. Protein type: Ubiquitin ligase; Ligase; EC 6.3.2.19; EC 6.3.2.-; Ubiquitin conjugating system. Chromosomal Location of Human Ortholog: 11p15. Cellular Component: cytoplasm; cytosol. Molecular Function: identical protein binding; protein binding; protein homodimerization activity; zinc ion binding; ubiquitin-protein ligase activity; pattern recognition receptor activity; ligase activity. Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; viral reproduction; positive regulation of MAPKKK cascade; negative regulation of virion penetration into host cell; cytokine and chemokine mediated signaling pathway; innate immune response; positive regulation of transcription factor activity; pattern recognition receptor signaling pathway; defense response to virus; activation of innate immune response; regulation of lipopolysaccharide-mediated signaling pathway; activation of NF-kappaB transcription factor

size1 :

0.1 mg

price1 :

345 USD

more info or order :

MyBioSource product webpage