Podocalyxin; GCTM-2 antigen; Gp200; PC; PODXL; PCLP; PCLP1 | MyBioSource MBS1498612 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Podocalyxin; GCTM-2 antigen; Gp200; PC; PODXL; PCLP; PCLP1

catalog :

MBS1498612

quantity :

0.05 mg

price :

185 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, FACS, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot. Positive WB detected in: Hela whole cell lysate, HEK293 whole cell lysate. All lanes: PODXL antibody at 2.5 ug/ml. Secondary. Goat polyclonal to Mouse IgG at 1/5000 dilution. Predicted band size: 59, 56 kDa. Observed band size: 59 kDa.

image 2 :

Western Blot. Positive WB detected in: A549 whole cell lysate. All lanes: PODXL antibody at 2.5 ug/ml. Secondary. Goat polyclonal to Mouse IgG at 1/5000 dilution. Predicted band size: 59, 56 kDa. Observed band size: 59 kDa.

image 3 :

Western Blot. Positive WB detected in: Mouse brain tissue. All lanes: PODXL antibody at 0.43 ug/ml. Secondary. Goat polyclonal to Mouse IgG at 1/5000 dilution. Predicted band size: 59, 56 kDa. Observed band size: 59 kDa.

product information

catalog number :

MBS1498612

products type :

Antibody

products full name :

Podocalyxin; GCTM-2 antigen; Gp200; PC; PODXL; PCLP; PCLP1

products short name :

[Podocalyxin]

other names :

[podocalyxin isoform 1; Podocalyxin; podocalyxin; podocalyxin like; GCTM-2 antigen; Gp200; Podocalyxin-like protein 1; PC; PCLP-1]

other gene names :

[PODXL; PODXL; PC; PCLP; Gp200; PCLP-1; PCLP; PCLP1; PC; PCLP-1]

uniprot entry name :

PODXL_HUMAN

clonality :

Monoclonal

isotype :

IgG2b

host :

Mouse

reactivity :

Human. Other species are not tested. Please decide the specificity by homology

sequence length :

558

specificity :

Specific for PODXL denatured and native forms

purity :

>95%, Protein G Purified

form :

Liquid

storage stability :

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

tested application :

ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)

app notes :

Recommended dilution:. WB:1:500-1:5000. IHC:1:50-1:500. IF:1:50-1:200. IP:1:200-1:2000

image1 heading :

Western Blot (WB)

image2 heading :

Western Blot (WB)

image3 heading :

Western Blot (WB)

image4 heading :

Western Blot (WB)

image4 description :

Western Blot. Positive WB detected in: Rat brain tissue,Rat heart tissue,Rat kidney tissue. All lanes: PODXL antibody at 1.28 ug/ml. Secondary. Goat polyclonal to Mouse IgG at 1/5000 dilution. Predicted band size: 59, 56 kDa. Observed band size: 59 kDa.

image5 heading :

Immunohistochemistry (IHC)

image5 description :

Immunohistochemistry of paraffin-embedded human kidney tissue at dilution of 1:200.

other info1 :

Storage Buffer: Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4. Conjugate: Non-conjugated

other info2 :

Immunogen: Recombinant Human PODXL protein. Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-23904 / sc-21705 / sc-21706 / sc-23903

products description :

Involved in the regulation of both adhesion and cell morphology and cancer progression. Function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up initial epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells.

ncbi gi num :

66277202

ncbi acc num :

NP_001018121.1

ncbi gb acc num :

NM_001018111.2

uniprot acc num :

O00592

ncbi mol weight :

55,386 Da

ncbi summary :

This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin. [provided by RefSeq, Jul 2008]

uniprot summary :

PODXL: Involved in the regulation of both adhesion and cell morphology and cancer progression. Function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell- cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up inital epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR- dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells. Belongs to the podocalyxin family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Cell adhesion. Chromosomal Location of Human Ortholog: 7q32-q33. Cellular Component: apical plasma membrane; cytoplasm; extracellular space; filopodium; integral to plasma membrane; intracellular membrane-bound organelle; lamellipodium; lipid raft; microvillus membrane; plasma membrane; ruffle. Molecular Function: protein binding. Biological Process: cell adhesion; cell migration; leukocyte migration; negative regulation of cell adhesion; negative regulation of cell-cell adhesion; positive regulation of cell migration; positive regulation of cell-cell adhesion mediated by integrin; regulation of microvillus biogenesis

size1 :

0.05 mg

price1 :

185 USD

size2 :

0.1 mg

price2 :

265

more info or order :

MyBioSource product webpage