Rabbit anti-Escherichia coli cAMP-activated global transcriptional regulator CRP polyclonal Antibody | MyBioSource MBS1490450 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit anti-Escherichia coli cAMP-activated global transcriptional regulator CRP polyclonal Antibody

catalog :

MBS1490450

quantity :

0.05 mg

price :

160 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

Escherichia coli str. K-12 substr. MG1655

application :

ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS1490450

products type :

Antibody

products full name :

Rabbit anti-Escherichia coli cAMP-activated global transcriptional regulator CRP polyclonal Antibody

products short name :

[cAMP-activated global transcriptional regulator CRP]

products name syn :

[Catabolite activator protein; CAP; Catabolite gene activator; cAMP receptor protein; CRP; cAMP regulatory protein; crp; cap; csm; b3357; JW5702]

other names :

[cAMP-activated global transcription factor, mediator of catabolite repression; cAMP-activated global transcriptional regulator CRP; cAMP-activated global transcription factor, mediator of catabolite repression; Catabolite activator protein; CAP; Catabolite gene activator; cAMP receptor protein; CRP; cAMP regulatory protein]

products gene name :

[CRP]

other gene names :

[crp; crp; cap; csm; ECK3345; JW5702; cap; csm; CAP; CRP]

uniprot entry name :

CRP_ECOLI

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Escherichia coli

sequence length :

210

purity :

Caprylic Acid Ammonium Sulfate Precipitation Purified

storage stability :

Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

tested application :

ELISA (EIA)

other info1 :

Storage Buffer: Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4. Conjugate: Non-conjugated

other info2 :

Immunogen: Recombinant Escherichia coli cAMP-activated global transcriptional regulator CRP protein

products description :

A global transcription regulator. Complexes with cyclic AMP (cAMP) which allosterically activates DNA binding (to consensus sequence 5'-AAATGTGATCTAGATCACATTT-3') to directly regulate the transcription of about 300 genes in about 200 operons and indirectly regulate the expression of about half the genome. There are 3 classes of CRP promoters; class I promoters have a single CRP-binding site upstream of the RNA polymerase (RNAP)-binding site, whereas in class II promoters the single CRP- and RNAP-binding site overlap, CRP making multiple contacts with RNAP. Class III promoters require multiple activator molecules, including at least one CRP dimer. It can act as an activator, repressor, coactivator or corepressor. Induces a severe bend in DNA (about 87 degrees), bringing upstream promoter elements into contact with RNAP. Acts as a negative regulator of its own synthesis as well as for adenylate cyclase (cyaA), which generates cAMP. High levels of active CRP are detrimental to growth.Plays a major role in carbon catabolite repression (CCR). CCR involves cAMP, adenylate cyclase (cyaA), CRP and the EIIA-Glc component of the PTS (crr). In the presence of glucose EIIA-Glc is dephosphorylated, and does not activate adenylate cyclase, leading to reduced cAMP and thus decreased CRP activity. Also plays a role in many other processes

ncbi gi num :

16131236

ncbi acc num :

NP_417816.1

ncbi gb acc num :

NC_000913.3

uniprot acc num :

P0ACJ8

ncbi mol weight :

23,640 Da

ncbi pathways :

Two-component System Pathway (1114); Two-component System Pathway (437)

ncbi summary :

[More information is available at EcoCyc: EG10164].

uniprot summary :

A global transcription regulator. Complexes with cyclic AMP (cAMP) which allosterically activates DNA binding (to consensus sequence 5'-AAATGTGATCTAGATCACATTT-3') to directly regulate the transcription of about 300 genes in about 200 operons and indirectly regulate the expression of about half the genome. There are 3 classes of CRP promoters; class I promoters have a single CRP-binding site upstream of the RNA polymerase (RNAP)-binding site, whereas in class II promoters the single CRP- and RNAP-binding site overlap, CRP making multiple contacts with RNAP. Class III promoters require multiple activator molecules, including at least one CRP dimer. It can act as an activator, repressor, coactivator or corepressor. Induces a severe bend in DNA (about 87 degrees), bringing upstream promoter elements into contact with RNAP. Acts as a negative regulator of its own synthesis as well as for adenylate cyclase (cyaA), which generates cAMP. High levels of active CRP are detrimental to growth (PubMed:16260780). Plays a major role in carbon catabolite repression (CCR). CCR involves cAMP, adenylate cyclase (cyaA), CRP and the EIIA-Glc component of the PTS (crr). In the presence of glucose EIIA-Glc is dephosphorylated, and does not activate adenylate cyclase, leading to reduced cAMP and thus decreased CRP activity. Also plays a role in many other processes (see PubMed:22573269).

size1 :

0.05 mg

price1 :

160 USD

size2 :

0.1 mg

price2 :

235

more info or order :

MyBioSource product webpage