Recombinant Mouse Cathepsin-B, Active | MyBioSource MBS146955 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Mouse Cathepsin-B, Active

catalog :

MBS146955

quantity :

0.002 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS146955

products type :

Recombinant Protein

products full name :

Recombinant Mouse Cathepsin-B, Active

products short name :

[Cathepsin-B]

products name syn :

[CTSB Mouse, Active; Cathepsin B; Cathepsin B1; CTSB; Cathepsin-B Mouse Recombinant, Active]

other names :

[cathepsin B preproprotein; Cathepsin B; cathepsin B; cathepsin B; Cathepsin B1]

products gene name :

[CTSB]

other gene names :

[Ctsb; Ctsb; CB]

sequence length :

339

sequence :

HDKPSFHPLS DDLINYINKQ NTTWQAGRNF YNVDISYLKK LCGTVLGGPK LPGRVAFGED IDLPETFDAR EQWSNCPTIG QIRDQGSCGS CWAFGAVEAI SDRTCIHTNG RVNVEVSAED LLTCCGIQCG DGCNGGYPSG AWSFWTKKGL VSGGVYNSHV GCLPYTIPPC EHHVNGSRPP CTGEGDTPRC NKSCEAGYSP SYKEDKHFGY TSYSVSNSVK EIMAEIYKNG PVEGAFTVFS DFLTYKSGVY KHEAGDMMGG HAIRILGWGV ENGVPYWLAA NSWNLDWGDN GFFKILRGEN HCGIESEIVA GIPRTDQYWG RF LEHHHHHH

purity :

Greater than 90% as determined by SDS-PAGE.

form :

Sterile filtered clear solution. CTSB protein solution (0.5mg/ml) containing Phosphate Buffered Saline (pH 7.4) and 10% glycerol.

storage stability :

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze thaw cycles

other info1 :

Source: Sf9, Baculovirus cells

other info2 :

Biological Activity: Specific activity is > 2, 000 pmol/min/ug and is defined as the amount of enzyme that hydrolyze 1pmole of Z-Arg-Arg-AMC to Z-Arg-Arg and AMC per minute at pH 6.0 at 37C.

products categories :

ENZYMES

products description :

CTSB Mouse Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 330 amino acids (18-339a.a.) and having a molecular mass of 36.4kDa (Molecular size on SDS-PAGE will appear at approximately 28-40kDa). CTSB is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

ncbi gi num :

6681079

ncbi acc num :

NP_031824.1

ncbi gb acc num :

NM_007798.3

uniprot acc num :

P10605

ncbi mol weight :

37,280 Da

ncbi pathways :

Antigen Processing And Presentation Pathway (83271); Antigen Processing And Presentation Pathway (485); Apoptosis Pathway (83257); Apoptosis Pathway (470); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (1367336); Collagen Degradation Pathway (1367348); Collagen Formation Pathway (1367334); Degradation Of The Extracellular Matrix Pathway (1367346); Extracellular Matrix Organization Pathway (1367333); Immune System Pathway (1367472)

ncbi summary :

This gene encodes a member of the peptidase C1 family and preproprotein that is proteolytically processed to generate multiple protein products. These products include the cathepsin B light and heavy chains, which can dimerize to generate the double chain form of the enzyme. This enzyme is a lysosomal cysteine protease with both endopeptidase and exopeptidase activity that may play a role in protein turnover. Homozygous knockout mice for this gene exhibit reduced pancreatic damage following induced pancreatitis and reduced hepatocyte apoptosis in a model of liver injury. Pseudogenes of this gene have been identified in the genome. [provided by RefSeq, Aug 2015]

uniprot summary :

CTSB: Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis. Dimer of a heavy chain and a light chain cross-linked by a disulfide bond. Interacts with SRPX2. Belongs to the peptidase C1 family. Protein type: Autophagy; EC 3.4.22.1; Motility/polarity/chemotaxis; Protease. Chromosomal Location of Human Ortholog: 14 D1 14 33.24 cM. Cellular Component: apical plasma membrane; caveola; cell surface; cytoplasm; external side of plasma membrane; extracellular region; extracellular space; intracellular membrane-bound organelle; lysosome; mitochondrion; nucleolus; perinuclear region of cytoplasm; sarcolemma. Molecular Function: collagen binding; cysteine-type endopeptidase activity; cysteine-type peptidase activity; endopeptidase activity; kininogen binding; peptidase activity; peptide binding; protein complex binding; protein self-association; proteoglycan binding. Biological Process: collagen catabolic process; decidualization; entry of virus into host cell; proteolysis; proteolysis involved in cellular protein catabolic process

size1 :

0.002 mg

price1 :

140 USD

size2 :

0.01 mg

price2 :

205

size3 :

0.1 mg

price3 :

1220

more info or order :

MyBioSource product webpage