protein

Recombinant Enhanced Green Fluorescent Protein

MBS146512

0.005 mg

140 USD

Recombinant Protein

Recombinant Enhanced Green Fluorescent Protein

[Enhanced Green Fluorescent Protein]

[Green fluorescent protein, GFP.]

[enhanced green fluorescent protein]

[EGFP]

Escherichia coli

239

MVSKGEELFT GVVPILVELD GDVNGHKFSV SGEGEGDATY GKLTLKFICT TGKLPVPWPT LVTTLTYGVQ CFSRYPDHMK QHDFFKSAMP EGYVQERTIF FKDDGNYKTR AEVKFEGDTL VNRIELKGID FKEDGNILGH KLEYNYNSHN VYIMADKQKN GIKVNFKIRH NIEDGSVQLA DHYQQNTPIG DGPVLLPDNH YLSTQSALSK DPNEKRDHMV LLEFVTAAGI TLGMDELYK

Greater than 95.0% as determined by:. (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE

The EGFP was lyophilized from a 0.2 um filtered concentrated solution in PBS pH 7.4

Lyophilized EGFP although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution EGFP should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Solubility: It is recommended to reconstitute the lyophilized EGFP in sterile distilled H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.

RECOMBINANT & NATURAL PROTEINS; Recombinant Proteins

Description: Recombinant EGFP produced in E Coli cells is a non-glycosylated, homodimeric protein containing 239 amino acid chain and having a molecular mass of 26.9kDa. EGFP is purified by proprietary chromatographic techniques. Introduction: GFP, also known as Green Fluorescent Protein, is a protein produced by the jellyfish (Aequorea Victoria) that produces bioluminescence in the green zone of the noticeable spectrum. Green Fluorescent Protein is a useful and ubiquitous instrument for producing chimeric proteins, where it functions as a fluorescent protein tag. GFP is expressed in most known cell types and is used as a noninvasive fluorescent marker in living cells and organisms. Green Fluorescent Protein permits a broad range of applications where it has functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Enhanced GFP (eGFP) has F64L and S65T mutations, which make GFP show increased fluorescence and fold more efficiently under 370.

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0.005 mg

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