Recombinant Human Interleukin-27 p28 | MyBioSource MBS146491 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human Interleukin-27 p28

catalog :

MBS146491

quantity :

0.001 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS146491

products type :

Recombinant Protein

products full name :

Recombinant Human Interleukin-27 p28

products short name :

[Interleukin-27 p28]

products name syn :

[Interleukin-30; IL-30; IL-27/p28; p28; Interleukin-27; Interleukin-27/p28; IL-27; Interleukin-27 subunit alpha; IL-27 subunit alpha; IL27-A; Il27; Il27a; IL-27p28]

other names :

[interleukin-27 subunit alpha; Interleukin-27 subunit alpha; interleukin-27 subunit alpha; IL-27 p28 subunit; IL-27 subunit alpha; IL-27-A; IL27-A; interleukin 30; interleukin-30; interleukin 27; Interleukin-30; p28]

products gene name :

[IL 27]

other gene names :

[IL27; IL27; p28; IL30; IL-27; IL27A; IL-27A; IL27p28; IL27A; IL30; IL-27 subunit alpha; IL-27-A; IL27-A]

uniprot entry name :

IL27A_HUMAN

host :

HEK

sequence length :

243

sequence :

IL-27A (215aa):FPRPPGRPQL SLQELRREFT VSLHLARKLL AEVRGQAHRF AESHLPGVNL YLLPLGEQLP DVSLTFQAWR RLSDPERLCF ISTTLQPFHA LLGGLGTQGR WTNMERMQLW AMRLDLRDLQ RHLRFQVLAA GFNLPEEEEE EEEEEEEERK GLLPGALGSA LQGPAQVSWP QLLSTYRLLH SLELVLSRAV RELLLLSKAG HSVWPLGFPT LSPQPIL-27B (209aa):RKGPPAALTL PRVQCRASRY PIAVDCSWTL PPAPNSTSPV SFIATYRLGM AARGHSWPCL QQTPTSTSCT ITDVQLFSMA PYVLNVTAVH PWGSSSSFVP FITEHIIKPD PPEGVRLSPL AERQLQVQWE PPGSWPFPEI FSLKYWIRYK RQGAARFHRV GPIEATSFIL RAVRPRARYY VQVAAQDLTD YGELSDWSLP ATATMSLGK

purity :

Greater than 90% as determined by analysis by SDS-PAGE.

form :

The IL-27 was lyophilized from 1mg/ml in 1xPBS.

storage stability :

Lyophilized IL-27 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL-27 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

other info1 :

Physical Appearance: Sterile Filtered White Lyophilized (freeze-dried powder).

other info2 :

Solubility: It is recommended to reconstitute the lyophilized IL-27 in sterile PBS containing 0.1% endotoxin-free recombinant HSA. Biological Activity: The specific activity was determined by the dose dependent inhibition of HIV replication in human CD4+ T cells and in human monocyte-derived macrophages and is typically 4-12ng/ml.

products categories :

CYTOKINES AND GROWTH FACTORS; Cytokines; Interleukin

products description :

IL-27 Human recombinant produced in HEK cells is a glycosylated non-covalently heterodimer that consists of 2 chains, IL27A (IL27p28) having a Mw of 25kDa and IL27B (EBl3) having a Mw OF 30 kDa. Each chain migrates separately on SDS-PAGE. The IL-27 protein is purified by propietary chromatographic techniques.

ncbi gi num :

28416913

ncbi acc num :

NP_663634.2

ncbi gb acc num :

NM_145659.3

uniprot acc num :

Q8NEV9

ncbi pathways :

IL27-mediated Signaling Events Pathway (137929)

ncbi summary :

The protein encoded by this gene is one of the subunits of a heterodimeric cytokine complex. This protein is related to interleukin 12A (IL12A). It interacts with Epstein-Barr virus induced gene 3 (EBI3), a protein similar to interleukin 12B (IL12B), and forms a complex that has been shown to drive rapid expansion of naive but not memory CD4(+) T cells. The complex is also found to synergize strongly with interleukin 12 to trigger interferon gamma (IFNG) production of naive CD4(+) T cells. The biological effects of this cytokine are mediated by the class I cytokine receptor (WSX1/TCRR). [provided by RefSeq, Jul 2008]

uniprot summary :

IL27-alpha: Cytokine with pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon- gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR which appears to be required but not sufficient for IL-27-mediated signal transduction. IL-27 potentiate the early phase of TH1 response and suppress TH2 and TH17 differentiation. It induces the differentiation of TH1 cells via two distinct pathways, p38 MAPK/TBX21- and ICAM1/ITGAL/ERK- dependent pathways. It also induces STAT1, STAT3, STAT4 and STAT5 phosphorylation and activates TBX21/T-Bet via STAT1 with resulting IL12RB2 up-regulation, an event crucial to TH1 cell commitment. It suppresses the expression of GATA3, the inhibitor TH1 cells development. In CD8 T-cells, it activates STATs as well as GZMB. IL-27 reveals to be a potent inhibitor of TH17 cell development and of IL-17 production. Indeed IL-27 subunit p28 alone is also able to inhibit the production of IL17 by CD4 and CD8 T-cells. While IL-27 suppressed the development of proinflammatory Th17 cells via STAT1, it inhibits the development of anti-inflammatory inducible regulatory T-cells, iTreg, independently of STAT1. IL-27 has also an effect on cytokine production, it suppresses proinflammatory cytokine production such as IL2, IL4, IL5 and IL6 and activates suppressors of cytokine signaling such as SOCS1 and SOCS3. Apart from suppression of cytokine production, IL-27 also antagonizes the effects of some cytokines such as IL6 through direct effects on T-cells. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines such as IP- 10/CXCL10 and MIG/CXCL9. In vein endothelial cells, it induces IRF1/interferon regulatory factor 1 and increase the expression of MHC class II transactivator/CIITA with resulting up-regulation of major histocompatibility complex class II. IL-27 also demonstrates antiviral activity with inhibitory properties on HIV-1 replivation. Belongs to the IL-6 superfamily. Protein type: Secreted, signal peptide; Cytokine; Secreted. Chromosomal Location of Human Ortholog: 16p11. Cellular Component: extracellular space. Molecular Function: interleukin-27 receptor binding; cytokine activity; receptor binding. Biological Process: regulation of T-helper 1 cell differentiation; response to bacterium; regulation of defense response to virus; regulation of T cell proliferation; innate immune response; inflammatory response; positive regulation of interferon-gamma biosynthetic process

size1 :

0.001 mg

price1 :

140 USD

size2 :

0.005 mg

price2 :

205

size3 :

0.1 mg

price3 :

1510

more info or order :

MyBioSource product webpage