protein

Recombinant Human Selectin P Ligand

MBS146316

0.002 mg

140 USD

Recombinant Protein

Recombinant Human Selectin P Ligand

[Selectin P Ligand]

[Cutaneous Lymphocyte-Associated Associated Antigen, Selectin P Ligand, PSGL-1, CD162 Antigen, P-Selectin Glycoprotein Ligand 1, CLA]

[Selectin P ligand; P-selectin glycoprotein ligand 1; P-selectin glycoprotein ligand 1; cutaneous lymphocyte-associated associated antigen; selectin P ligand; Selectin P ligand; CD_antigen: CD162]

[SELPLG]

[SELPLG; SELPLG; CLA; CD162; PSGL1; PSGL-1; PSGL-1]

SELPL_HUMAN

402

MGSSHHHHHH SSGLVPRGSH MGS QATEYEY LDYDFLPETE PPEMLRNSTD TTPLTGPGTP ESTTVEPAAR RSTGLDAGGA VTELTTELAN MGNLSTDSAA MEI

Greater than 80% as determined by SDS-PAGE.

The SELPLG solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 1mM DTT and 10% glycerol.

0.25mg/ml

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

Source: Escherichia Coli.

Physical Appearance: Sterile Filtered clear solution.

RECOMBINANT & NATURAL PROTEINS

Introduction:. SELPLG glycoprotein functions as a high affinity counter-receptor for the cell adhesion selectin molecules (P, E and L) located in stimulated T lymphocytes and myeloid cells. SELPLG binds leukocytes to activated platelets or endothelia expressing selectins, a vital role in leukocyte trafficking throughout inflammation. In order to have a high-affinity binding activity SELPLG needs two post-translational modifications, tyrosine sulfation and the addition of the sialyl Lewis x tetrasaccharide (sLex) to its O-linked glycans. Polymorphisms and abnormal expression of SELPLG are linked to defects in the innate and adaptive immune response. Alternate splicing results in multiple transcript variants. Description:. SELPLG Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 103 amino acids (42-121) and having a molecular mass of 10.9 kDa (Molecular size on SDS-PAGE will appear higher). SELPLG is fused to a 23 amino acid His-tag at N-terminus.

20987422

AAH29782.1

Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Cell Surface Interactions At The Vascular Wall Pathway (106062); Hemostasis Pathway (106028); Staphylococcus Aureus Infection Pathway (172846); Staphylococcus Aureus Infection Pathway (171867); Amb2 Integrin Signaling Pathway (137945)

This gene encodes a glycoprotein that functions as a high affinity counter-receptor for the cell adhesion molecules P-, E- and L- selectin expressed on myeloid cells and stimulated T lymphocytes. As such, this protein plays a critical role in leukocyte trafficking during inflammation by tethering of leukocytes to activated platelets or endothelia expressing selectins. This protein requires two post-translational modifications, tyrosine sulfation and the addition of the sialyl Lewis x tetrasaccharide (sLex) to its O-linked glycans, for its high-affinity binding activity. Aberrant expression of this gene and polymorphisms in this gene are associated with defects in the innate and adaptive immune response. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Apr 2011]

SELPLG: A SLe(x)-type glycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. PSGL1 is critical for the initial leukocyte capture. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 12q24. Cellular Component: membrane; integral to plasma membrane; plasma membrane; integral to membrane. Molecular Function: protein binding; receptor binding. Biological Process: cell adhesion; blood coagulation; leukocyte tethering or rolling; leukocyte migration; leukocyte adhesive activation

0.002 mg

140 USD

0.01 mg

205

0.1 mg

1220