Recombinant Human Dystroglycan 1 DAG1 Human | MyBioSource MBS146082 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human Dystroglycan 1 DAG1 Human

catalog :

MBS146082

quantity :

0.002 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS146082

products type :

Recombinant Protein

products full name :

Recombinant Human Dystroglycan 1 DAG1 Human

products short name :

[Dystroglycan 1]

products name syn :

[Dystroglycan, Dystrophin-associated glycoprotein 1, DAG1, A3a, DAG, AGRNR, 156DAG, MDDGC7, MDDGC9.]

other names :

[dystroglycan preproprotein; Dystroglycan; dystroglycan; dystroglycan 1 (dystrophin-associated glycoprotein 1); Dystrophin-associated glycoprotein 1Cleaved into the following 2 chains:Alpha-dystroglycan; Alpha-DG; Beta-dystroglycan; Beta-DG]

products gene name :

[DAG1]

other gene names :

[DAG1; DAG1; A3a; DAG; AGRNR; 156DAG; MDDGC7; MDDGC9; Alpha-DG; Beta-DG]

uniprot entry name :

DAG1_HUMAN

sequence length :

895

sequence :

MKHHHHHHAS HWPSEPSEAV RDWENQLEAS MHSVLSDLHE AVPTVVGIPD GTAVVGRSFR VTIPTDLIAS SGDIIKVSAA GKEALPSWLH WDSQSHTLEG LPLDTDKGVH YISVSATRLG ANGSHIPQTS SVFSIEVYPE DHSELQSVRT ASPDPGEVVS SACAADEPVT VLTVILDADL TKMTPKQRID LLHRMRSFSE VELHNMKLVP VVNNRLFDMS AFMAGPGNAK KVVENGALLS WKLGCSLNQN SVPDIHGVEA PAREGAMSAQ LGYPVVGWHI ANKKPPLPKR VRR.

purity :

Greater than 90.0% as determined by SDS-PAGE.

form :

DAG1 filtered (0.4 um) and lyophilized from 0.5mg/ml in 0.05M phosphate buffer and 0.075M NaCl, pH 7.4. Filtered White lyophilized (freeze-dried) powder.

storage stability :

Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

other info1 :

Source: Escherichia Coli.

other info2 :

Solubility: It is recommended to add 200 ul deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. DAG1 is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

products categories :

RECOMBINANT & NATURAL PROTEINS; Recombinant Proteins

products description :

Description: DAG1 Human Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain (a.a 30-312) containing 293 amino acids including a 10 a.a N-terminal His tag. The total molecular mass is 31.87kDa (calculated). Introduction: Dystroglycan 1 (DAG1) is a laminin binding component of the dystrophin-glycoprotein complex which provides a connection between the subsarcolemmal cytoskeleton and the extracellular matrix. The N-terminal domain of alpha-dystroglycan is secreted into the cerebrospinal fluid. The effect of DAG1 on the nervous system remains vague. The complete dystroglycan complex is expressed in a various tissues and has a role in processes such as laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization. DAG1 is a candidate gene for the site of the mutation in autosomal recessive muscular dystrophies. The dramatic decrease of DAG1 in Duchenne muscular dystrophy leads to a loss of linkage between the sarcolemma and extracellular matrix, making muscle fibers more susceptible to necrosis.

ncbi gi num :

294997284

ncbi acc num :

NP_001159400.2

ncbi gb acc num :

NM_001165928.3

uniprot acc num :

Q14118

ncbi mol weight :

97,441 Da

ncbi pathways :

Arrhythmogenic Right Ventricular Cardiomyopathy Pathway (672454); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (117293); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (116129); Dilated Cardiomyopathy Pathway (121494); Dilated Cardiomyopathy Pathway (121285); ECM Proteoglycans Pathway (833812); ECM-receptor Interaction Pathway (83068); ECM-receptor Interaction Pathway (479); Extracellular Matrix Organization Pathway (576262); Hypertrophic Cardiomyopathy (HCM) Pathway (114229)

ncbi summary :

Dystroglycan is a laminin binding component of the dystrophin-glycoprotein complex which provides a linkage between the subsarcolemmal cytoskeleton and the extracellular matrix. Dystroglycan 1 is a candidate gene for the site of the mutation in autosomal recessive muscular dystrophies. The dramatic reduction of dystroglycan 1 in Duchenne muscular dystrophy leads to a loss of linkage between the sarcolemma and extracellular matrix, rendering muscle fibers more susceptible to necrosis. Dystroglycan also functions as dual receptor for agrin and laminin-2 in the Schwann cell membrane. The muscle and nonmuscle isoforms of dystroglycan differ by carbohydrate moieties but not protein sequence. Alternative splicing results in multiple transcript variants all encoding the same protein.[provided by RefSeq, Apr 2010]

uniprot summary :

DAG1: a cytoskeletal protein that functions as a laminin receptor. Provides a linkage between the subsarcolemmal cytoskeleton and the extracellular matrix. May be involved in autosomal recessive muscular dystrophies. Its dramatic reduction in Duchenne muscular dystrophy leads to a loss of linkage between the sarcolemma and extracellular matrix, rendering muscle fibers more susceptible to necrosis. Dystroglycan also functions as dual receptor for agrin and laminin-2 in the Schwann cell membrane. Binds to several types of arenaviruses. Is a target for the entry of Mycobacterium leprae into peripheral nerve Schwann cells. The muscle and nonmuscle isoforms of dystroglycan differ by carbohydrate moieties but not protein sequence. Protein type: Cytoskeletal; Membrane protein, integral; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 3p21. Cellular Component: dystroglycan complex; extracellular space; costamere; focal adhesion; basolateral plasma membrane; extracellular region; integral to membrane; lipid raft; nucleoplasm; dystrophin-associated glycoprotein complex; postsynaptic membrane; cytoskeleton; cell-cell adherens junction; lamellipodium; cytoplasm; plasma membrane; basement membrane; sarcolemma; filopodium. Molecular Function: tubulin binding; laminin-1 binding; viral receptor activity; protein binding; structural constituent of muscle; protein complex binding; calcium ion binding; SH2 domain binding; alpha-actinin binding; actin binding; vinculin binding. Biological Process: nerve maturation; response to peptide hormone stimulus; extracellular matrix organization and biogenesis; entry of virus into host cell; negative regulation of protein kinase B signaling cascade; negative regulation of MAPKKK cascade; cytoskeletal anchoring; membrane protein ectodomain proteolysis; NLS-bearing substrate import into nucleus; myelination in the peripheral nervous system; virus-host interaction; morphogenesis of an epithelial sheet; calcium-dependent cell-matrix adhesion; negative regulation of cell migration. Disease: Muscular Dystrophy-dystroglycanopathy (limb-girdle), Type C, 9; Muscular Dystrophy-dystroglycanopathy (congenital With Brain And Eye Anomalies), Type A, 9

size1 :

0.002 mg

price1 :

140 USD

size2 :

0.01 mg

price2 :

205

size3 :

1 mg

price3 :

5015

more info or order :

MyBioSource product webpage