protein

Recombinant Human Ectonucleotide Pyrophosphatase

MBS146019

0.002 mg

140 USD

Recombinant Protein

Recombinant Human Ectonucleotide Pyrophosphatase

[Ectonucleotide Pyrophosphatase]

[Ectonucleotide pyrophosphatase/phosphodiesterase family member 1, E-NPP 1, Membrane component chromosome 6 surface marker 1, Phosphodiesterase I/nucleotide pyrophosphatase 1, Plasma-cell membrane glycoprotein PC-1, ENPP1, M6S1, NPPS, PC1, PDNP1, NPP1, PC-1, PCA1, ARHR2, COLED]

[ectonucleotide pyrophosphatase/phosphodiesterase family member 1; Ectonucleotide pyrophosphatase/phosphodiesterase family member 1; ectonucleotide pyrophosphatase/phosphodiesterase family member 1; E-NPP 1; Ly-41 antigen; alkaline phosphodiesterase 1; membrane component chromosome 6 surface marker 1; membrane component, chromosome 6, surface marker 1; phosphodiesterase I/nucleotide pyrophosphatase 1; plasma-cell membrane glycoprotein 1; plasma-cell membrane glycoprotein PC-1; ectonucleotide pyrophosphatase/phosphodiesterase 1; Membrane component chromosome 6 surface marker 1; Phosphodiesterase I/nucleotide pyrophosphatase 1; Plasma-cell membrane glycoprotein PC-1Including the following 2 domains:Alkaline phosphodiesterase I (EC:3.1.4.1); Nucleotide pyrophosphatase (EC:3.6.1.9); NPPase]

[ENPP1]

[ENPP1; ENPP1; M6S1; NPP1; NPPS; PC-1; PCA1; ARHR2; COLED; PDNP1; M6S1; NPPS; PC1; PDNP1; E-NPP 1; NPPase]

ENPP1_HUMAN

925

AS KPSCAKEV KSCKGRCFER TFGNCRCDAA CVELGNCCLD YQETCIEPEH IWTCNKFRCG EKRLTRSLCA CSDDCKDKGD CCINYSSVCQ GEKSWVEEPC ESINEPQCPA GFETPPTLLF SLDGFRAEYL HTWGGLLPVI SKLKKCGTYT KNMRPVYPTK TFPNHYSIVT GLYPESHGII DNKMYDPKMN ASFSLKSKEK FNPEWYKGEP IWVTAKYQGL KSGTFFWPGS DVEINGIFPD IYKMYNGSVP FEERILAVLQ WLQLPKDERP HFYTLYLEEP DSSGHSYGPV SSEVIKALQR VDGMVGMLMD GLKELNLHRC LNLILISDHG MEQGSCKKYI YLNKYLGDVK NIKVIYGPAA RLRPSDVPDK YYSFNYEGIA RNLSCREPNQ HFKPYLKHFL PKRLHFAKSD RIEPLTFYLD PQWQLALNPS ERKYCGSGFH GSDNVFSNMQ ALFVGYGPGF KHGIEADTFE NIEVYNLMCD LLNLTPAPNN GTHGSLNHLL KNPVYTPKHP KEVHPLVQCP FTRNPRDNLG CSCNPSILPI EDFQTQFNLT VAEEKIIKHE TLPYGRPRVL QKENTICLLS QHQFMSGYSQ DILMPLWTSY TVDRNDSFST EDFSNCLYQD FRIPLSPVHK CSFYKNNTKV SYGFLSPPQL NKNSSGIYSE ALLTTNIVPM YQSFQVIWRY FHDTLLRKYA EERNGVNVVS GPVFDFDYDG RCDSLENLRQ KRRVIRNQEI LIPTHFFIVL TSCKDTSQTP LHCENLDTLA FILPHRTDNS ESCVHGKHDS SWVEELLMLH RARITDVEHI TGLSFYQQRK EPVSDILKLK THLPTFSQED GPKLHHHHHH .

Greater than 95% as determined by SDS-PAGE

Filtered (0.4 um) and lyophilized from 0.5mg/ml in 0.05M phosphate buffer and 0.075M NaCl, pH 7.4. Filtered lyophilized (freeze-dried) powder.

Store lyophilized protein at -20 degree C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4 degree C for a limited period of time.

Source: HEK 293. Solubility: It is recommended to add 200ul deionized water to a working concentration of 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

ENZYMES; Enzymes; Phosphatase

Description: ENPP1 Human Recombinant produced in HEK cells is a single, glycosylated, polypeptide chain (a.a 98-925) containing a total of 840 amino acids, having a molecular mass of 96.5kDa (calculated) though it migrates at approximately 110kDa on SDS PAGE, the ENPP1 is also composed of a 2 a.a N-terminal linker, a 4 a.a C-terminal linker and fused to a 6 a.a His tag at C-Terminus.The Human ENPP1 is purified by proprietary chromatographic techniques. Introduction: Ectonucleotide Pyrophosphatase (ENPP1) belongs to the ecto-nucleotide pyrophosphatase/phosphodiesterase (ENPP) family. ENPP1 is a type II transmembrane glycoprotein comprised of 2 identical disulfide-bonded subunits. The ENPP1 protein has broad specificity and cleaves various substrates, including phosphodiester bonds of nucleotides and nucleotide sugars and pyrophosphate bonds of nucleotides and nucleotide sugars. The ENPP1 protein can hydrolyze nucleoside 5' triphosphates to their corresponding monophosphates and it may also hydrolyze diadenosine polyphosphates. ENPP1 gene mutations are linked with 'idiopathic' infantile arterial calcification, ossification of the posterior longitudinal ligament of the spine (OPLL), and insulin resistance.

170650661

NP_006199.2

NM_006208.2

P22413

Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway (906000); Defective BTD Causes Biotidinase Deficiency Pathway (906015); Defective CD320 Causes Methylmalonic Aciduria Pathway (906012); Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway (906001); Defective GIF Causes Intrinsic Factor Deficiency Pathway (906004); Defective HLCS Causes Multiple Carboxylase Deficiency Pathway (906014); Defective LMBRD1 Causes Methylmalonic Aciduria And Homocystinuria Type CblF Pathway (906003); Defective MMAA Causes Methylmalonic Aciduria Type CblA Pathway (906010); Defective MMAB Causes Methylmalonic Aciduria Type CblB Pathway (906009); Defective MMACHC Causes Methylmalonic Aciduria And Homocystinuria Type CblC Pathway (906005)

This gene is a member of the ecto-nucleotide pyrophosphatase/phosphodiesterase (ENPP) family. The encoded protein is a type II transmembrane glycoprotein comprising two identical disulfide-bonded subunits. This protein has broad specificity and cleaves a variety of substrates, including phosphodiester bonds of nucleotides and nucleotide sugars and pyrophosphate bonds of nucleotides and nucleotide sugars. This protein may function to hydrolyze nucleoside 5' triphosphates to their corresponding monophosphates and may also hydrolyze diadenosine polyphosphates. Mutations in this gene have been associated with 'idiopathic' infantile arterial calcification, ossification of the posterior longitudinal ligament of the spine (OPLL), and insulin resistance. [provided by RefSeq, Jul 2008]

ENPP1: Involved primarily in ATP hydrolysis at the plasma membrane. Plays a role in regulating pyrophosphate levels, and functions in bone mineralization and soft tissue calcification. In vitro, has a broad specificity, hydrolyzing other nucleoside 5 triphosphates such as GTP, CTP, TTP and UTP to their corresponding monophosphates with release of pyrophosphate and diadenosine polyphosphates, and also 3 ,5 -cAMP to AMP. May also be involved in the regulation of the availability of nucleotide sugars in the endoplasmic reticulum and Golgi, and the regulation of purinergic signaling. Appears to modulate insulin sensitivity. Homodimer; disulfide-linked. Interacts with INSR. Expressed in plasma cells and also in a number of non-lymphoid tissues, including the distal convoluted tubule of the kidney, chondrocytes and epididymis. At low concentrations of ATP, a phosphorylated intermediate is formed which inhibits further hydrolysis. Belongs to the nucleotide pyrophosphatase/phosphodiesterase family. Protein type: Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Carbohydrate Metabolism - starch and sucrose; EC 3.6.1.9; Phosphodiesterase; Nucleotide Metabolism - purine; Phosphatase (non-protein); Cofactor and Vitamin Metabolism - riboflavin; Membrane protein, integral; Motility/polarity/chemotaxis; EC 3.1.4.1. Chromosomal Location of Human Ortholog: 6q22-q23. Cellular Component: extracellular space; cell surface; integral to plasma membrane; basolateral plasma membrane; lysosomal membrane; integral to membrane; plasma membrane. Molecular Function: phosphodiesterase I activity; protein binding; nucleotide diphosphatase activity; protein homodimerization activity; nucleic acid binding; 3 -phosphoadenosine 5 -phosphosulfate binding; zinc ion binding; calcium ion binding; nucleoside-triphosphate diphosphatase activity; scavenger receptor activity; insulin receptor binding; ATP binding; polysaccharide binding. Biological Process: receptor-mediated endocytosis; generation of precursor metabolites and energy; sequestering of triacylglycerol; vitamin metabolic process; nucleoside triphosphate catabolic process; negative regulation of insulin receptor signaling pathway; bone remodeling; negative regulation of fat cell differentiation; phosphate metabolic process; 3 -phosphoadenosine 5 -phosphosulfate metabolic process; riboflavin metabolic process; negative regulation of glucose import; cellular phosphate ion homeostasis; cellular response to insulin stimulus; biomineral formation; negative regulation of ossification; immune response; negative regulation of protein amino acid autophosphorylation; negative regulation of cell growth; regulation of bone mineralization; inorganic diphosphate transport; water-soluble vitamin metabolic process; negative regulation of glycogen biosynthetic process. Disease: Obesity; Cole Disease; Arterial Calcification, Generalized, Of Infancy, 1; Hypophosphatemic Rickets, Autosomal Recessive, 2; Diabetes Mellitus, Noninsulin-dependent

0.002 mg

140 USD

0.01 mg

205

1 mg

5015