Recombinant Human Lipoprotein Lipase, HEK | MyBioSource MBS145361 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human Lipoprotein Lipase, HEK

catalog :

MBS145361

quantity :

0.002 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS145361

products type :

Recombinant Protein

products full name :

Recombinant Human Lipoprotein Lipase, HEK

products short name :

[Lipoprotein Lipase]

products name syn :

[LPL Human, HEK; Lipoprotein Lipase Human Recombinant, HEK; Lipoprotein lipase; LPL; LIPD; HDLCQ11; LPL, HEK]

other names :

[lipoprotein lipase; Lipoprotein lipase; lipoprotein lipase; lipoprotein lipase]

products gene name :

[LPL]

other gene names :

[LPL; LPL; LIPD; HDLCQ11; LIPD; LPL]

uniprot entry name :

LIPL_HUMAN

host :

HEK293 cells

sequence length :

475

sequence :

HVDYKDDDDK PAG ADQRRDF IDIESKFALR TPEDTAEDTC HLIPGVAESV ATCHFNHSSK TFMVIHGWTV TGMYESWVPK ADQRRDF IDIESKFALR TPEDTAEDTC HLIPGVAESV ATCHFNHSSK TFMVIHGWTV TGMYESWVPKLVAALYKREP DSNVIVVDWL SRAQEHYPVS AGYTKLVGQD VARFINWMEE EFNYPLDNVH LLGYSLGAHA AGIAGSLTNK KVNRITGLDP AGPNFEYAEA PSRLSPDDAD FVDVLHTFTR GSPGRSIGIQ KPVGHVDIYP NGGTFQPGCN IGEAIRVIAE RGLGDVDQLV KCSHERSIHL FIDSLLNEEN PSKAYRCSSK EAFEKGLCLS CRKNRCNNLG YEISKVRAKR SSKMYLKTRS QMPYKVFHYQ VKIHFSGTES ETHTNQAFEI SLYGTVAESE NIPFTLPEVS TNKTYSFLIY TEVDIGELLM LKLKWKSDSY FSWSDWWSSP GFAIQKIRVK AGETQKKVIF CSREKVSHLQ KGKAPAVFVK CHDKSLNKKS G.

form :

LPL was filtered (0.4 um) and lyophilized from 0.5 mg/ml in 20mM Tris buffer and 50mM NaCl, pH 7.5. Filtered white lyophilized powder.

storage stability :

Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

other info1 :

Physical Appearance: Filtered white lyophilized powder. Solubility: It is recomended to add deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

products categories :

ENZYMES; Enzymes; Lipase

products description :

The Recombinant Human LPL produced in HEK293 cell line has a molecular mass of 51.8kDa containing 461 amino acid residues of the human LPL (Ala28-Gly475, variant Asn > Ser318) and fused to a 13 a.a. Flag-tag at N-terminus.

ncbi gi num :

4557727

ncbi acc num :

NP_000228.1

ncbi gb acc num :

NM_000237.2

uniprot acc num :

P06858

ncbi pathways :

Adipogenesis Pathway (198832); Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Alzheimers Disease Pathway (672448); Chylomicron-mediated Lipid Transport Pathway (106157); Developmental Biology Pathway (477129); Disease Pathway (530764); Diseases Associated With Visual Transduction Pathway (771581); Fatty Acid Beta Oxidation Pathway (198865); Glycerolipid Metabolism Pathway (82986)

ncbi summary :

LPL encodes lipoprotein lipase, which is expressed in heart, muscle, and adipose tissue. LPL functions as a homodimer, and has the dual functions of triglyceride hydrolase and ligand/bridging factor for receptor-mediated lipoprotein uptake. Severe mutations that cause LPL deficiency result in type I hyperlipoproteinemia, while less extreme mutations in LPL are linked to many disorders of lipoprotein metabolism. [provided by RefSeq, Jul 2008]

uniprot summary :

LPL: The primary function of this lipase is the hydrolysis of triglycerides of circulating chylomicrons and very low density lipoproteins (VLDL). Binding to heparin sulfate proteogylcans at the cell surface is vital to the function. The apolipoprotein, APOC2, acts as a coactivator of LPL activity in the presence of lipids on the luminal surface of vascular endothelium. Defects in LPL are the cause of lipoprotein lipase deficiency (LPL deficiency); also known as familial chylomicronemia or hyperlipoproteinemia type I. LPL deficiency chylomicronemia is a recessive disorder usually manifesting in childhood. On a normal diet, patients often present with abdominal pain, hepatosplenomegaly, lipemia retinalis, eruptive xanthomata, and massive hypertriglyceridemia, sometimes complicated with acute pancreatitis. Belongs to the AB hydrolase superfamily. Lipase family. Protein type: Membrane protein, GPI anchor; Phospholipase; EC 3.1.1.34; Lipid Metabolism - glycerolipid. Chromosomal Location of Human Ortholog: 8p22. Cellular Component: extracellular matrix; extracellular space; chylomicron; cell surface; plasma membrane; extracellular region. Molecular Function: heparin binding; triacylglycerol lipase activity; protein binding; lipoprotein lipase activity; apolipoprotein binding; phospholipase activity; triglyceride binding; receptor binding. Biological Process: response to drug; phototransduction, visible light; triacylglycerol metabolic process; phospholipid metabolic process; triacylglycerol catabolic process; triacylglycerol biosynthetic process; lipoprotein metabolic process; response to cold; retinoid metabolic process; fatty acid biosynthetic process. Disease: Hyperlipoproteinemia, Type I; Hyperlipidemia, Familial Combined

size1 :

0.002 mg

price1 :

140 USD

size2 :

0.01 mg

price2 :

205

size3 :

1 mg

price3 :

5775

more info or order :

MyBioSource product webpage