catalog number :
MBS145257
products type :
Recombinant Protein
products full name :
Recombinant E Coli Ribonuclease H1
products short name :
Ribonuclease H1
products name syn :
RNASEH1 E.Coli; Ribonuclease H1 E.Coli Recombinant; Ribonuclease HI; RNase HI; Ribonuclease H; RNase H; rnhA; dasF; herA; rnh; sdrA; b0214; JW0204
other names :
ribonuclease HI, degrades RNA of DNA-RNA hybrids; Ribonuclease HI; ribonuclease HI, degrades RNA of DNA-RNA hybrids; Ribonuclease H; RNase H
products gene name :
RNASEH1
other gene names :
rnhA; rnhA; cer; dasF; ECK0214; herA; JW0204; rnh; sdrA; sin; dasF; herA; rnh; sdrA; RNase HI; RNase H
uniprot entry name :
RNH_ECOLI
purity :
Greater than 95% as determined by SDS-PAGE.
form :
RNASEH1 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 10% glycerol and 2mM DTT. Sterile Filtered clear solution.
products categories :
ENZYMES; Enzymes
products description :
Description: RNASEH1 E Coli Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 178 amino acids (1-155 a.a.) and having a molecular mass of 20kDa.RNASEH1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. Introduction: RNHA is an endonuclease which specifically degrades the RNA of RNA-DNA hybrids. Localized to the nucleus, the RNHA protein mediates the removal of Okazaki fragment RNA primers which are present on the lagging strand during DNA replication. RNHA catalyzes the endonucleolytic cleavage of RNA to a 5'-phosphomonoester and is capable of binding magnesium or manganese as cofactors.
ncbi acc num :
NP_414750.1
ncbi gb acc num :
NC_000913.3
ncbi mol weight :
17,597 Da
ncbi pathways :
DNA Replication Pathway (1122); DNA Replication Pathway (444)
ncbi summary :
RNase H cleaves RNA in RNA-DNA hybrids. [More information is available at EcoCyc: EG10860].