protein

Recombinant Human Catalase

MBS145120

0.005 mg

140 USD

Recombinant Protein

Recombinant Human Catalase

Catalase

CAT Human; Catalase Human Recombinant; Catalase; CAT

catalase; Catalase; catalase; catalase

CAT

CAT; CAT

CATA_HUMAN

E Coli

527

MGSSHHHHHH SSGLVPRGSH MADSRDPASD QMQHWKEQRA AQKADVLTIG AGNPVGDKLN VITVGPRGPL LVQDVVFTDE MAHFDRERIP ERVVHAKGAG AFGYFEVTHD ITKYSKAKVF FHIGKKTPIA VRFSTVAGES GSADTVRDPR GFAVKFYTED GNWDLVGNNT PIFFIRDPIL FPSFIHSQKR NPQTHLKDPD MVWDFWSLRP ESLHQVSFLF SDRGIPDGHR HMNGYGSHTF KLVNANGEAV YCKFHYKTDQ GIKNLSVEDA ARLSQEDPDY GIRDLFNAIA TGKYPSWTFY IQVMTFNQAE TFPFNPFDLT KVWPHKDYPL IPVGKLVLNR NPVNYFAEVE QIAFDPSNMP PGIEASPDKM LQGRLFAYPD THRHRLGPNY LHIPVNCPYR ARVANYQRDG PMCMQDNQGG APNYYPNSFG APEQQPSALE HSIQYSGEVR RFNTANDDNV TQVRAFYVNV LNEEQRKRLC ENIAGHLKDA QIFIQKKAVK NFTEVHPDYG SHIQALLDKY NAEKPKNAIH TFVQSGSHLA AREKANL.

Greater than 90.0% as determined by SDS-PAGE.

The CAT solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl and 10% glycerol. Sterile filtered yellowish solution.

Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree Cfor longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or B5A). Avoid multiple freeze-thaw cycles.

Unit Defenition: One unit will decompose 1.0 umole of H2O2 per minute at pH 8.0 at 25 degree C.

Biological Activity: Specific activity is >30,000 unit/mg.

ENZYMES; Enzymes

Description: CAT Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 547 amino acids (1-527) and having a molecular mass of 61.9kDa.CAT is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. Introduction: Catalase (CAT) is a key antioxidant enzyme in the body's defense against oxidative stress. Furthermore, Catalase is a heme enzyme which is present in the peroxisome of virtually all aerobic cells. Catalase converts the reactive oxygen species hydrogen peroxide to water and oxygen and thus diminishes the toxic effects of hydrogen peroxide. Catalase stimulates growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells. Catalase gene polymorphisms are linked with decreases in catalase activity nevertheless, to date, acatalasemia is the only disease known to be caused by the CAT gene.

4557014

NP_001743.1

NM_001752.3

P04040

Amyotrophic Lateral Sclerosis (ALS) Pathway (920975); Amyotrophic Lateral Sclerosis (ALS) Pathway (83099); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Cellular Responses To Stress Pathway (645258); DNA Damage Response (only ATM Dependent) Pathway (198827); Detoxification Of Reactive Oxygen Species Pathway (1127552); Folate Metabolism Pathway (198833); FoxO Family Signaling Pathway (138036); FoxO Signaling Pathway (921162); Glyoxylate And Dicarboxylate Metabolism Pathway (83002)

This gene encodes catalase, a key antioxidant enzyme in the bodies defense against oxidative stress. Catalase is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. Catalase converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Oxidative stress is hypothesized to play a role in the development of many chronic or late-onset diseases such as diabetes, asthma, Alzheimer's disease, systemic lupus erythematosus, rheumatoid arthritis, and cancers. Polymorphisms in this gene have been associated with decreases in catalase activity but, to date, acatalasemia is the only disease known to be caused by this gene. [provided by RefSeq, Oct 2009]

Catalase: Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells. Homotetramer. Belongs to the catalase family. Protein type: Endoplasmic reticulum; Oxidoreductase; Amino Acid Metabolism - tryptophan; Apoptosis; Energy Metabolism - methane; EC 1.11.1.6; Hydrolase; Mitochondrial. Chromosomal Location of Human Ortholog: 11p13. Cellular Component: Golgi apparatus; peroxisomal membrane; peroxisomal matrix; focal adhesion; membrane; intracellular membrane-bound organelle; endoplasmic reticulum; lysosome; plasma membrane; mitochondrial intermembrane space; peroxisome; cytosol. Molecular Function: antioxidant activity; oxidoreductase activity, acting on peroxide as acceptor; protein homodimerization activity; enzyme binding; catalase activity; metal ion binding; heme binding; NADP binding; aminoacylase activity; receptor binding. Biological Process: cholesterol metabolic process; nucleobase, nucleoside and nucleotide metabolic process; hemoglobin metabolic process; purine base metabolic process; activation of NF-kappaB transcription factor; protein homotetramerization; positive regulation of phosphoinositide 3-kinase cascade; osteoblast differentiation; response to vitamin E; response to reactive oxygen species; triacylglycerol metabolic process; response to hyperoxia; UV protection; inhibition of NF-kappaB transcription factor; ureteric bud development; hydrogen peroxide catabolic process; positive regulation of cell division; aerobic respiration; response to hypoxia; purine nucleotide catabolic process; protein tetramerization; negative regulation of apoptosis; aging. Disease: Acatalasemia

0.005 mg

140 USD

0.02 mg

205

1 mg

2665