Recombinant Human Cyclin-B1 | MyBioSource MBS145097 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human Cyclin-B1

catalog :

MBS145097

quantity :

0.002 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS145097

products type :

Recombinant Protein

products full name :

Recombinant Human Cyclin-B1

products short name :

Cyclin-B1

products name syn :

G2/mitotic-specific cyclin-B1, cyclin B1, CCNB.

other names :

G2/mitotic-specific cyclin-B1; G2/mitotic-specific cyclin-B1; G2/mitotic-specific cyclin-B1; G2/mitotic-specific cyclin B1; cyclin B1

products gene name :

CCNB1

other gene names :

CCNB1; CCNB1; CCNB; CCNB

uniprot entry name :

CCNB1_HUMAN

host :

E Coli

sequence length :

433

sequence :

MGSSHHHHHH SSGLVPRGSH MGSHMALRVT RNSKINAENK AKINMAGAKR VPTAPAATSK PGLRPRTALG DIGNKVSEQL QAKMPMKKEA KPSATGKVID KKLPKPLEKV PMLVPVPVSE PVPEPEPEPE PEPVKEEKLS PEPILVDTAS PSPMETSGCA PAEEDLCQAF SDVILAVNDV DAEDGADPNL CSEYVKDIYA YLRQLEEEQA VRPKYLLGRE VTGNMRAILI DWLVQVQMKF RLLQETMYMT VSIIDRFMQN NCVPKKMLQL VGVTAMFIAS KYEEMYPPEI GDFAFVTDNT YTKHQIRQME MKILRALNFG LGRPLPLHFL RRASKIGEVD VEQHTLAKYL MELTMLDYDM VHFPPSQIAA GAFCLALKIL DNGEWTPTLQ HYLSYTEESL LPVMQHLAKN VVMVNQGLTK HMTVKNKYAT SKHAKISTLP QLNSALVQDL AKAVAKV.

purity :

Greater than 80% as determined by SDS-PAGE.

form :

The CCNB1 solution (0.25mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 150mM NaCl and 10% glycerol. Sterile Filtered colorless solution.

storage stability :

Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

products categories :

PROTEIN KINASES; Enzymes; Cyclin

products description :

Description: CCNB1 Human Recombinant produced in E Coli is a single polypeptide chain containing 457 amino acids (1-433) and having a molecular mass of 50.9 kDa.CCNB1 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. Introduction: Cyclin B1 (CCNB1) is a regulatory protein involved in mitosis. CCNB1 creates a complex with p34(cdc2) to form the maturation-promoting factor (MPF). CCNB1 is vital for the control of the cell cycle at the G2/M (mitosis) transition. CCNB1 builds up steadily during the G2 and is immediately destroyed at mitosis. The 2 alternative transcripts produce a constitutively expressed transcript and a cell cycle-regulated transcript which is expressed predominantly during G2/M phase. These transcripts are a result of alternate transcription initiation sites.

ncbi gi num :

14327896

ncbi acc num :

NP_114172.1

ncbi gb acc num :

NM_031966.3

uniprot acc num :

P14635

ncbi pathways :

AMPK Signaling Pathway (198868); APC/C-mediated Degradation Of Cell Cycle Proteins Pathway (105825); APC/C:Cdc20 Mediated Degradation Of Cyclin B Pathway (105832); APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway (105831); ATM Signaling Pathway (920969); Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway (105829); Activation Of NIMA Kinases NEK9, NEK6, NEK7 Pathway (771571); C-MYB Transcription Factor Network Pathway (138073); Cell Cycle Pathway (530733); Cell Cycle Checkpoints Pathway (105739)

ncbi summary :

The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008]

uniprot summary :

CCNB1: a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Complexes with cdc2 to form the maturation-promoting factor (MPF). Two alternatively spliced isoforms have been found, a constitutively expressed form and a cell cycle-regulated form that is expressed predominantly during G2/M phase. Protein type: Activator; Cell cycle regulation. Chromosomal Location of Human Ortholog: 5q12. Cellular Component: nucleoplasm; spindle pole; centrosome; membrane; cytoplasm; cytosol; nucleus. Molecular Function: protein binding; histone kinase activity; patched binding; protein kinase binding. Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of histone phosphorylation; regulation of cell cycle; ventricular cardiac muscle cell development; mitotic nuclear envelope disassembly; positive regulation of attachment of spindle microtubules to kinetochore; positive regulation of mRNA 3 -end processing; positive regulation of mitotic cell cycle; positive regulation of fibroblast proliferation; negative regulation of protein amino acid phosphorylation; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; tissue regeneration; protein complex assembly; G2/M transition of mitotic cell cycle; positive regulation of cardiac muscle cell proliferation; response to drug; in utero embryonic development; oocyte maturation; mitotic metaphase plate congression; response to DDT; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; gut development; cell division; response to mechanical stimulus; spermatogenesis; regulation of cyclin-dependent protein kinase activity; mitotic cell cycle; mitotic spindle stabilization; G1/S transition of mitotic cell cycle

size1 :

0.002 mg

price1 :

140 USD

size2 :

0.01 mg

price2 :

205

size3 :

0.1 mg

price3 :

1220

more info or order :

MyBioSource product webpage