protein

Recombinant Human nth Endonuclease III-Like 1

MBS145085

0.002 mg

140 USD

Recombinant Protein

Recombinant Human nth Endonuclease III-Like 1

nth Endonuclease III-Like 1

NTHL1 Human; Nth Endonuclease III-Like 1 Human Recombinant; Endonuclease III-like protein 1; NTHL1; NTH1; OCTS3

endonuclease III-like protein 1; Endonuclease III-like protein 1; endonuclease III-like protein 1; DNA glycoslyase/AP lyase; NTHL1; bifunctional DNA N-glycoslyase/DNA-(apurinic or apyrimidinic site) lyase; nth endonuclease III-like 1 (E. coli); Bifunctional DNA N-glycoslyase/DNA-(apurinic or apyrimidinic site) lyase

NTHL1

NTHL1; NTHL1; NTH1; OCTS3; hNTH1; hNTH1

NTH_HUMAN

E Coli

312

Greater than 80.0% as determined by SDS-PAGE.

NTHL1 protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 40% glycerol, 0.15M NaCl and 1mM DTT. Sterile filtered colorless solution.

Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

ENZYMES; Enzymes; Endonuclease

Description: NTHL1 Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 332 amino acids (1-312 a.a.) and having a molecular mass of 36.6kDa.NTHL1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. Introduction: Nth endonuclease III-like protein 1 (NTHL1) is one of the enzymes which act on a large number of oxidatively damaged DNA sites. NTHL1 has an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. NTHL1 acts on a damaged strand, 5' from the damaged site and incises damaged DNA at cytosines, thymines and guanines.

4505471

NP_002519.1

NM_002528.5

P78549

32,839 Da

Base Excision Repair Pathway (105838); Base Excision Repair Pathway (83043); Base Excision Repair Pathway (451); Base-Excision Repair, AP Site Formation Pathway (105839); Base-free Sugar-phosphate Removal Via The Single-nucleotide Replacement Pathway (105848); Cleavage Of The Damaged Pyrimidine Pathway (105845); DNA Repair Pathway (105837); Depyrimidination Pathway (105843); Displacement Of DNA Glycosylase By APE1 Pathway (105849); Recognition And Association Of DNA Glycosylase With Site Containing An Affected Pyrimidine Pathway (105844)

The protein encoded by this gene is a DNA N-glycosylase of the endonuclease III family. Like a similar protein in E. coli, the encoded protein has DNA glycosylase activity on DNA substrates containing oxidized pyrimidine residues and has apurinic/apyrimidinic lyase activity. [provided by RefSeq, Oct 2008]

NTHL1: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5 from the damaged site. Required for the repair of both oxidative DNA damage and spontaneous mutagenic lesions. Belongs to the Nth/MutY family. Protein type: DNA repair, damage; Hydrolase; Mitochondrial; EC 4.2.99.18; Lyase. Chromosomal Location of Human Ortholog: 16p13.3. Cellular Component: nucleoplasm; mitochondrion; nucleus. Molecular Function: protein binding; DNA-(apurinic or apyrimidinic site) lyase activity; endonuclease activity; metal ion binding; 4 iron, 4 sulfur cluster binding; double-stranded DNA binding; DNA N-glycosylase activity. Biological Process: base-excision repair, AP site formation; base-excision repair; depyrimidination; DNA repair; nucleotide-excision repair, DNA incision, 5 -to lesion; DNA catabolic process, endonucleolytic. Disease: Familial Adenomatous Polyposis 3

0.002 mg

140 USD

0.01 mg

205

1 mg

5015