Recombinant Human Chromodomain Helicase DNA Binding Protein 4 | MyBioSource MBS144735 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human Chromodomain Helicase DNA Binding Protein 4

catalog :

MBS144735

quantity :

0.005 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS144735

products type :

Recombinant Protein

products full name :

Recombinant Human Chromodomain Helicase DNA Binding Protein 4

products short name :

Chromodomain Helicase DNA Binding Protein 4

products name syn :

CHD4 Human; Chromodomain Helicase DNA Binding Protein 4 Human Recombinant; Chromodomain Helicase DNA binding protein 4; Mi-2b; Mi2-BETA; CHD-4; ATP-dependent helicase CHD4; Mi-2 autoantigen 218 kDa protein; EC 3.6.4.12; EC 3.6.1

other names :

chromodomain-helicase-DNA-binding protein 4 isoform 1; Chromodomain-helicase-DNA-binding protein 4; chromodomain-helicase-DNA-binding protein 4; ATP-dependent helicase CHD4; Mi-2 autoantigen 218 kDa protein; chromodomain helicase DNA binding protein 4; ATP-dependent helicase CHD4; Mi-2 autoantigen 218 kDa protein; Mi2-beta

products gene name :

CHD4

other gene names :

CHD4; CHD4; CHD-4; Mi-2b; Mi2-BETA; CHD-4

uniprot entry name :

CHD4_HUMAN

host :

Sf9 Insect Cells

sequence length :

1912

purity :

Greater than 95% as determined by SDS-PAGE.

form :

CHD4 (0.25mg/ml) is supplied in 20mM HEPES buffer pH-8.0 and 500mM NaCl.

concentration :

0.25mg/ml

storage stability :

Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. Avoid multiple freeze-thaw cycles.

tested application :

Western Blot (WB), ELISA (EIA), Immunodot

app notes :

Western blot with myositis sera ormonoclonal anti-hexa-His-tag antibody.

other info1 :

Description: CHD4 is a full-length cDNA coding for the human Mi-2 beta isoform having a molecular mass of 221,298 Dalton (pH 5.8). CHD4 protein is fused to a hexa-histidine purification tag. Coating Concentration: 0.3-0.7 ug/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination. Immunological Tests: Standard ELISA test (checkerboard analysis of positive/negative sera panels); Immunodot test with positive/negative sera panels. Physical Appearance: Sterile filtered colorless solution.

other info2 :

Immunological Functions: Binds IgG-type human auto-antibodies.

products categories :

RECOMBINANT & NATURAL PROTEINS; Recombinant Proteins

products description :

Introduction: CHD4 is a member of a family of alleged chromodomain helicase-DNA-binding proteins. Biochemically, CHD4 is a component of the nucleosome transformation and deacetylase (NuRD) complex that takes part in transcription regulation. Autoantibodies targeting the CHD4 are a serologic feature of idiopathic inflammatory myopathies (IIM). In IIM Mi-2 antibodies are characterized by diagnostic sensitivity and specificity of approximately 4-18% and 98-100%, respectively. Moreover, anti-CHD4 antibodies are related to dermatomyositis (frequency up to 31%) and have a great positive predictive value for this type of disease subset. Anti-CHD4 are the only defined myositis-specific autoantibodies clearly focused to a nuclear target. An additional slightly outstanding feature of Mi-2 antibodies relates to their frequency in children, which is similar to that in adults.

ncbi gi num :

51599156

ncbi acc num :

NP_001264.2

ncbi gb acc num :

NM_001273.3

uniprot acc num :

Q14839

ncbi mol weight :

220,848 Da

ncbi pathways :

Chromatin Modifying Enzymes Pathway (1127682); Chromatin Organization Pathway (1127681); Gene Expression Pathway (105937); HDACs Deacetylate Histones Pathway (1127684); RNA Polymerase I Promoter Clearance Pathway (106551); RNA Polymerase I Transcription Pathway (106550); RNA Polymerase I Transcription Initiation Pathway (106553); RNA Polymerase I, RNA Polymerase III, And Mitochondrial Transcription Pathway (160948); Signaling Events Mediated By HDAC Class I Pathway (137997); Viral Carcinogenesis Pathway (658418)

ncbi summary :

The product of this gene belongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein. Somatic mutations in this gene are associated with serous endometrial tumors. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2014]

uniprot summary :

CHD-4: is an ATP-dependent helicase and a central component of the HDAC-containing nucleosome remodeling and histone deacetylase (NuRD) transcriptional complex. Interacts with MTA1/2, HDAC1, and RbAp46 in the NuRD complex. CHD-4 belongs to the SNF2/RAD54 helicase family of proteins. The NuRD complex, whose subunit composition varies by cell type and in response to physiologic signals, plays central roles in many processes including oncogenesis and metastasis, B cell differentiation, invasive growth of breast cancer, and maintaining the balance between the undifferentiated state and the process of differentiation in embryonic stem cells. Protein type: EC 3.6.4.12; DNA-binding; Helicase. Chromosomal Location of Human Ortholog: 12p13. Cellular Component: nucleoplasm; centrosome; protein complex; membrane; nuclear chromatin; cytoplasm; NuRD complex; nucleus. Molecular Function: ATP-dependent DNA helicase activity; protein binding; nucleosomal DNA binding; DNA binding; zinc ion binding; microtubule binding; ATP binding. Biological Process: regulation of transcription from RNA polymerase II promoter; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; ATP-dependent chromatin remodeling; spindle assembly; DNA duplex unwinding

size1 :

0.005 mg

price1 :

140 USD

size2 :

0.02 mg

price2 :

205

size3 :

1 mg

price3 :

3775

more info or order :

MyBioSource product webpage