Recombinant Human Lysyl-tRNA Synthetase | MyBioSource MBS144584 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human Lysyl-tRNA Synthetase

catalog :

MBS144584

quantity :

0.005 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS144584

products type :

Recombinant Protein

products full name :

Recombinant Human Lysyl-tRNA Synthetase

products short name :

[Lysyl-tRNA Synthetase]

products name syn :

[KARS Human; Lysyl-tRNA Synthetase Human Recombinant; Lysine--tRNA ligase; Lysyl-tRNA synthetase; LysRS; KARS; KIAA0070; KRS; KARS2; CMTRIB]

other names :

[lysine--tRNA ligase isoform 1; Lysine--tRNA ligase; lysine--tRNA ligase; lysRS; lysine tRNA ligase; lysyl-tRNA synthetase; Lysyl-tRNA synthetase; LysRS]

products gene name :

[KARS]

other gene names :

[KARS; KARS; KRS; KARS1; KARS2; CMTRIB; DFNB89; KIAA0070; LysRS]

uniprot entry name :

SYK_HUMAN

sequence length :

625

sequence :

MGSSHHHHHH SSGLVPRGSH MGS GVGPEEE SVDPNQYYKI RSQAIHQLKV NGEDPYPHKF HVDISLTDFI QKYSHLQPGD HLTDITLKVA GRIHAKRASG GKLIFYDLRG EGVKLQVMAN SRNYKSEEEF IHINNKLRRG DIIGVQGNPG KTKKGELSII PYEITLLSPC LHMLPHLHFG LKDKETRYRQ RYLDLILNDF VRQKFIIRSK IITYIRSFLD ELGFLEIETP MMNIIPGGAV AKPFITYHNE LDMNLYMRIA PELYHKMLVV GGIDRVYEIG RQFRNEGIDL THNPEFTTCE FYMAYADYHD LMEITEKMVS GMVKHITGSY KVTYHPDGPE GQAYDVDFTP PFRRINMVEE LEKALGMKLP ETNLFETEET RKILDDICVA KAVECPPPRT TARLLDKLVG EFLEVTCINP TFICDHPQIM SPLAKWHRSK EGLTERFELF VMKKEICNAY TELNDPMRQR QLFEEQAKAK AAGDDEAMFI DENFCTALEY GLPPTAGWGM GIDRVAMFLT DSNNIKEVLL FPAMKPEDKK ENVATTDTLE STTVGTSV.

purity :

Greater than 95.0% as determined by SDS-PAGE.

form :

KARS protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1M NaCl and 1mM DTT. Sterile Filtered colorless solution.

storage stability :

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

other info1 :

Source: Escherichia Coli.

products categories :

ENZYMES; Enzymes; Synthetase

products description :

Description: KARS Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 558 amino acids (63-597 a.a.) and having a molecular mass of 63.7kDa.KARS is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. Introduction: Lysyl-tRNA synthetase (KARS) is a member of the class-II aminoacyl-tRNA synthetase family. KARS exists as both mitochondrial and cytoplasmic isoforms produced by alternative splicing, and believed to have a role in autoimmune diseases, such as polymyositis or dermatomyositis. The KARS protein functions to catalyze the aminoacylation of tRNAs by their corresponding amino acids, so linking amino acids with tRNA-contained nucleotide triplets.

ncbi gi num :

194272210

ncbi acc num :

NP_001123561.1

ncbi gb acc num :

NM_001130089.1

uniprot acc num :

Q15046

ncbi mol weight :

71,497 Da

ncbi pathways :

Aminoacyl-tRNA Biosynthesis Pathway (83030); Aminoacyl-tRNA Biosynthesis Pathway (424); Aminoacyl-tRNA Biosynthesis, Eukaryotes Pathway (413439); Aminoacyl-tRNA Biosynthesis, Eukaryotes Pathway (468360); Cytosolic TRNA Aminoacylation Pathway (105981); Gene Expression Pathway (105937); Mitochondrial TRNA Aminoacylation Pathway (105982); TRNA Aminoacylation Pathway (105980)

ncbi summary :

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

KARS: Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of diadenosine oligophosphate (Ap4A), a signaling molecule involved in the activation of MITF transcriptional activity. Interacts with HIV-1 virus GAG protein, facilitating the selective packaging of tRNA(3)(Lys), the primer for reverse transcription initiation. Homodimer; also part of a multisubunit complex that groups AIMP1, AIMP2, EEF1A1 and tRNA ligases for Arg, Asp, Glu, Gln, Ile, Leu, Lys, Met and Pro. Interacts with AIMP2 (via N- terminus) and MITF. Interacts directly with HIV-1 virus GAG protein. Up-regulated by DARS and EEF1A1, but not by AIMP2. Belongs to the class-II aminoacyl-tRNA synthetase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: EC 6.1.1.6; Translation; Ligase; Mitochondrial. Chromosomal Location of Human Ortholog: 16q23.1. Cellular Component: microtubule cytoskeleton; aminoacyl-tRNA synthetase multienzyme complex; mitochondrion; mitochondrial matrix; cytoplasm; extracellular region; plasma membrane; nucleus; cytosol. Molecular Function: amino acid binding; protein binding; metal ion binding; lysine-tRNA ligase activity; ATP binding; tRNA binding. Biological Process: tRNA aminoacylation for protein translation; lysyl-tRNA aminoacylation; viral reproduction; tRNA processing; gene expression; diadenosine tetraphosphate biosynthetic process. Disease: Deafness, Autosomal Recessive 89; Charcot-marie-tooth Disease, Recessive Intermediate B

size1 :

0.005 mg

price1 :

140 USD

size2 :

0.02 mg

price2 :

205

size3 :

1 mg

price3 :

2665

more info or order :

MyBioSource product webpage