protein

Recombinant Human POU Class 5 Homeobox 1

MBS144551

0.005 mg

140 USD

Recombinant Protein

Recombinant Human POU Class 5 Homeobox 1

POU Class 5 Homeobox 1

POU5F1 Human; POU Class 5 Homeobox 1 Human Recombinant; POU domain class 5 transcription factor 1; Octamer-binding protein 3; Oct-3; Octamer-binding protein 4; Oct-4; Octamer-binding transcription factor 3; OTF-3; POU5F1; OCT3; OCT4; OTF3; OTF4; MGC22487

POU domain, class 5, transcription factor 1 isoform 3; POU domain, class 5, transcription factor 1; POU domain, class 5, transcription factor 1; POU domain transcription factor OCT4; POU-type homeodomain-containing DNA-binding protein; octamer-binding protein 3; octamer-binding protein 4; octamer-binding transcription factor 3; POU class 5 homeobox 1; Octamer-binding protein 3; Oct-3; Octamer-binding protein 4; Oct-4; Octamer-binding transcription factor 3; OTF-3

POU5F1

POU5F1; POU5F1; OCT3; OCT4; OTF3; OTF4; OTF-3; Oct-3; Oct-4; OCT3; OCT4; OTF3; Oct-3; Oct-4; OTF-3

PO5F1_HUMAN

E Coli

265

Greater than 85% as determined by SDS-PAGE.

The POU5F1 solution contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol. Sterile filtered colorless solution.

RECOMBINANT & NATURAL PROTEINS; Recombinant Proteins; POU Class

Description: POU5F1 Human Recombinant produced in E Coli is a single polypeptide chain containing 285 amino acids (1-265) and having a molecular mass of 32.2 kDa.POU5F1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. Introduction: POU5F1 is a homeodomain transcription factor of the POU family, expressed in embryonic stem (ES) cells and embryonic carcinoma (EC) cells.POU5F1 is significantly involved in the signaling pathway for maintaining self-renewal and pluripotency of ES cells. POU5F1 has 2 distinct DNA binding domains which independently bind half-sites of the canonical octamer motif. This flexibility allows POU5F1 to bind with distinct DNA motifs by forming heterodimers with other transcription factors or by forming homodimers in several conformations. Human POU5F1 contains a 75aa POU specific (POUS) domain and a 60aa POU-Homeo-(POUH) domain connected by a linker region. The Human POU5F1 specifically interacts with Octamer motif ATGCAAAT. In addition, 2 proline-rich domains in the N-terminal and C-terminal regions are vital for POU5F1 transactivation. POU5F1 regulates a number of target genes and has been shown to work jointly with other transcription factors including Sox2 as well as Nanog to sustain stem cell potency and self-renewal.

553727233

NP_001272916.1

NM_001285987.1

Q01860

30,085 Da

Cardiac Progenitor Differentiation Pathway (712094); Developmental Biology Pathway (477129); HIF-2-alpha Transcription Factor Network Pathway (137956); POU5F1 (OCT4), SOX2, NANOG Activate Genes Related To Proliferation Pathway (1127697); POU5F1 (OCT4), SOX2, NANOG Repress Genes Related To Differentiation Pathway (1127698); SIDS Susceptibility Pathways (198901); Signaling Pathways Regulating Pluripotency Of Stem Cells (1026136); Signaling Pathways Regulating Pluripotency Of Stem Cells (1033502); Transcriptional Regulation Of Pluripotent Stem Cells Pathway (1127696); Wnt Signaling Pathway And Pluripotency (198847)

This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq, Oct 2013]

Oct4: Transcription factor that binds to the octamer motif (5 -ATTTGCAT-3 ). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. Interacts with UBE2I and ZSCAN10. Interacts with PKM2. Interacts with WWP2. Transcriptional activity is positively regulated by PKM2. Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues. Belongs to the POU transcription factor family. Class- 5 subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Transcription factor; DNA-binding. Chromosomal Location of Human Ortholog: 6p21.31. Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; cytosol; nucleus. Molecular Function: protein binding; miRNA binding; DNA binding; sequence-specific DNA binding; ubiquitin protein ligase binding; transcription factor binding; transcription factor activity. Biological Process: transcription from RNA polymerase II promoter; anatomical structure morphogenesis; blastocyst development; regulation of transcription, DNA-dependent; regulation of asymmetric cell division; regulation of gene expression; somatic stem cell maintenance; endodermal cell fate specification; response to wounding; positive regulation of transcription from RNA polymerase II promoter; mRNA transcription from RNA polymerase II promoter

0.005 mg

140 USD

0.02 mg

205

1 mg

3490