Recombinant Human Dihydrolipoamide S-Succinyltransferase | MyBioSource MBS144463 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human Dihydrolipoamide S-Succinyltransferase

catalog :

MBS144463

quantity :

0.002 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS144463

products type :

Recombinant Protein

products full name :

Recombinant Human Dihydrolipoamide S-Succinyltransferase

products short name :

Dihydrolipoamide S-Succinyltransferase

products name syn :

DLST Human; Dihydrolipoamide S-Succinyltransferase Human Recombinant; dihydrolipoamide S-succinyltransferase (E2 component of 2-oxo-glutarate complex); Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex; component E2; DLST; E2K; OGDC-E2; EC 2.3.1.61; EC 2.3.1

other names :

dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial isoform 1; Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial; dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial; E2K; OGDC-E2; dihydrolipoamide S-succinyltransferase (E2 component of 2-oxo-glutarate complex); 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex; E2K

products gene name :

DLST

other gene names :

DLST; DLST; DLTS; DLTS; OGDC-E2

uniprot entry name :

ODO2_HUMAN

host :

Sf9 Insect Cells

sequence length :

453

purity :

DLST purity was found to be greater than 75% as determined by SDS-PAGE.

form :

DLST (0.74mg/ml) is supplied in 20mM HEPES buffer pH-8.0, 200mM NaCl and 20% glycerol.

concentration :

0.4-1.0 ug/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.

tested application :

Western Blot (WB), ELISA (EIA), Immunodot

app notes :

Western blot with anti-M2-Antigen autoantibody-positive patient sera or monoclonal anti-hexa-His-tag antibody. Immunological Tests: Standard ELISA test (checkerboard analysis of positive/negative sera panels); immunodot test with positive/negative sera panels.

other info2 :

Immunological Functions: DLST binds IgG-type human auto-antibodies.

products categories :

ENZYMES; Enzymes; Transferase

products description :

Description: DLST is a Full-length cDNA coding for the mature form of the human OGDC-E2 protein having a molecular mass of 42,301 Dalton (pH 6.3).DLST protein is fused to a hexa-histidine purification tag. Introduction: DLST catalyzes the general transformation of 2-oxoglutarate to succinyl-CoA and CO(2). DLST holds multiple copies of 3 enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3).

ncbi gi num :

19923748

ncbi acc num :

NP_001924.2

ncbi gb acc num :

NM_001933.4

uniprot acc num :

P36957

ncbi mol weight :

39,553 Da

ncbi pathways :

2-oxoglutarate Decarboxylation To Succinyl-CoA Pathway (142336); 2-oxoglutarate Decarboxylation To Succinyl-CoA Pathway (139119); Carbon Metabolism Pathway (814926); Carbon Metabolism Pathway (817567); Citrate Cycle (TCA Cycle) Pathway (82927); Citrate Cycle (TCA Cycle) Pathway (288); Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway (855811); Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway (468202); Citrate Cycle, Second Carbon Oxidation, 2-oxoglutarate = Oxaloacetate Pathway (413348); Citrate Cycle, Second Carbon Oxidation, 2-oxoglutarate = Oxaloacetate Pathway (468204)

ncbi summary :

This gene encodes a mitochondrial protein that belongs to the 2-oxoacid dehydrogenase family. This protein is one of the three components (the E2 component) of the 2-oxoglutarate dehydrogenase complex that catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2011]

uniprot summary :

DLST: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3). Forms a 24-polypeptide structural core with octahedral symmetry. Belongs to the 2-oxoacid dehydrogenase family. Protein type: Mitochondrial; Transferase; EC 2.3.1.61; Carbohydrate Metabolism - citrate (TCA) cycle; Amino Acid Metabolism - lysine degradation. Chromosomal Location of Human Ortholog: 14q24.3. Cellular Component: membrane; mitochondrial matrix; oxoglutarate dehydrogenase complex; nucleus. Molecular Function: dihydrolipoyllysine-residue succinyltransferase activity. Biological Process: cellular metabolic process; generation of precursor metabolites and energy; tricarboxylic acid cycle; L-lysine catabolic process to acetyl-CoA via saccharopine; lysine catabolic process

size1 :

0.002 mg

price1 :

140 USD

size2 :

0.01 mg

price2 :

205

size3 :

1 mg

price3 :

3965

more info or order :

MyBioSource product webpage