Recombinant Mouse Ciliary Neurotrophic Factor | MyBioSource MBS144453 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Mouse Ciliary Neurotrophic Factor

catalog :

MBS144453

quantity :

0.005 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS144453

products type :

Recombinant Protein

products full name :

Recombinant Mouse Ciliary Neurotrophic Factor

products short name :

Ciliary Neurotrophic Factor

products name syn :

CNTF Mouse; Ciliary-Neurotrophic Factor Mouse Recombinant; HCNTF; CNTF; Ciliary Neurotrophic Factor

other names :

ciliary neurotrophic factor; Ciliary neurotrophic factor; ciliary neurotrophic factor; ciliary neurotrophic factor

products gene name :

mCNTF

other gene names :

Cntf; Cntf; AI429687; CNTF

uniprot entry name :

CNTF_MOUSE

host :

E Coli

sequence length :

198

sequence :

MAFAEQSPLT LHRRDLCSRS IWLARKIRSD LTALMESYVK HQGLNKNISL DSVDGVPVAS TDRWSEMTEA ERLQENLQAY RTFQGMLTKL LEDQRVHFTP TEGDFHQAIH TLTLQVSAFA YQLEELMALL EQKVPEKEAD GMPVTIGDGG LFEKKLWGLK VLQELSQWTV RSIHDLRVIS SHHMGISAHE SHYGAKQM

purity :

Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

form :

Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4. Sterile Filtered White lyophilized (freeze-dried) powder.

storage stability :

Lyophilized Ciliary Neurotrophic Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution CNTF should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.Please prevent freeze-thaw cycles.

other info2 :

Solubility: It is recommended to reconstitute the lyophilized CNTF in sterile 18M-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of TF-1 cells is less than 35ng/ml, corresponding to a Specific Activity of 3.0x10 4 IU/mg.

products categories :

CYTOKINES AND GROWTH FACTORS; Neurotrophins; Ciliary-Neurotrophic Factor

products description :

Description: Ciliary Neurotrophic Factor Recombinant Mouse produced in E Coli is a single, non-glycosylated polypeptide chain containing 198 amino acids and having a molecular mass of 22.6kDa. The CNTF is purified by proprietary chromatographic techniques. Introduction: CNTF is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. In addition to the predominant monocistronic transcript originating from this locus, the gene is also co-transcribed with the upstream ZFP91 gene. Co-transcription from the two loci results in a transcript that contains a complete coding region for the zinc finger protein but lacks a complete coding region for ciliary neurotrophic factor.CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.

ncbi gi num :

25952141

ncbi acc num :

NP_740756.1

ncbi gb acc num :

NM_170786.2

uniprot acc num :

P51642

ncbi mol weight :

22,587 Da

ncbi pathways :

Cytokine-cytokine Receptor Interaction Pathway (83248); Cytokine-cytokine Receptor Interaction Pathway (460); Delta-Notch Signaling Pathway (198316); Jak-STAT Signaling Pathway (83274); Jak-STAT Signaling Pathway (488)

ncbi summary :

The protein encoded by this gene is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes, and it may be involved in reducing tissue destruction during inflammatory attacks. A read-through transcript variant composed of Zfp91 and Cntf sequences has been identified, but it is thought to be non-coding. Read-through transcription of Zfp91 and Cntf has been observed in both human and mouse. [provided by RefSeq, Aug 2008]

uniprot summary :

CNTF: CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy. Belongs to the CNTF family. Cellular Component: extracellular space; cell soma; axon; cytoplasm; nucleus; cytosol. Molecular Function: interleukin-6 receptor binding; growth factor activity; cytokine activity; ciliary neurotrophic factor receptor binding. Biological Process: negative regulation of photoreceptor cell differentiation; nervous system development; positive regulation of axon regeneration; protein heterooligomerization; multicellular organismal development; membrane hyperpolarization; positive regulation of hormone secretion; positive regulation of JAK-STAT cascade; JAK-STAT cascade; positive regulation of cell growth; positive regulation of tyrosine phosphorylation of Stat3 protein; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of MAPKKK cascade; positive regulation of cell proliferation; regulation of resting membrane potential; regulation of retinal cell programmed cell death; neuron development; negative regulation of neuron apoptosis; astrocyte activation; cell differentiation; muscle morphogenesis; growth

size1 :

0.005 mg

price1 :

140 USD

size2 :

0.025 mg

price2 :

205

size3 :

1 mg

price3 :

2665

more info or order :

MyBioSource product webpage