Recombinant Human Sirtuin-3 | MyBioSource MBS144309 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human Sirtuin-3

catalog :

MBS144309

quantity :

0.002 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS144309

products type :

Recombinant Protein

products full name :

Recombinant Human Sirtuin-3

products short name :

Sirtuin-3

products name syn :

SIRT3 Human; Sirtuin 3 Human Recombinant; Sirtuin 3; SIR2-like protein 3; Sir2-like 3; NAD-dependent deacetylase sirtuin-3; mitochondrial

other names :

NAD-dependent protein deacetylase sirtuin-3, mitochondrial isoform b; NAD-dependent protein deacetylase sirtuin-3, mitochondrial; NAD-dependent protein deacetylase sirtuin-3, mitochondrial; NAD-dependent deacetylase sirtuin-3, mitochondrial; SIR2-like protein 3; mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase; regulatory protein SIR2 homolog 3; silent mating type information regulation 2, S.cerevisiae, homolog 3; sir2-like 3; sirtuin type 3; sirtuin 3; Regulatory protein SIR2 homolog 3; SIR2-like protein 3

products gene name :

SIRT3

other gene names :

SIRT3; SIRT3; SIR2L3; SIR2L3; hSIRT3

uniprot entry name :

SIR3_HUMAN

host :

E Coli

sequence length :

257

purity :

Greater than 95% as determined by SDS-PAGE.

form :

The SIRT3 protein solution (1mg/1ml) is formulated in 20mM Tris-HCl buffer (pH8.0) 1mM DTT, 0.1M NaCl, 10% glycerol. Sterile Filtered clear solution.

concentration :

1mg/ml

storage stability :

Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

products categories :

RECOMBINANT & NATURAL PROTEINS; Recombinant Proteins; Sirtuin

products description :

Description: SIRT3 produced in E Coli is a single, non-glycosylated polypeptide chain containing 303 amino acids (118-399a.a.) and having a molecular mass of 33.5kDa.SIRT3 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. Introduction: SIRT3 is a member of a family of proteins called Sirtuin. Proteins of the Sirtuin family are characterized by a Sirtuin core domain and grouped into four classes. The roles of human Sirtuins are numerous and are important in aging prosseses, stress resistance and metabolic regulation. SIRT3 shows NAD+-dependent deacetylase activity in the mitochondria. Over-expression of SIRT3 causes increased levels of the mitochondrial uncoupling protein 1. Also, in certain breast cancers the levels of SIRT3 protein are found to be extremely high.

ncbi gi num :

63054862

ncbi acc num :

NP_001017524.1

ncbi gb acc num :

NM_001017524.2

uniprot acc num :

Q9NTG7

ncbi mol weight :

28,567 Da

ncbi pathways :

Central Carbon Metabolism In Cancer Pathway (1059538); Central Carbon Metabolism In Cancer Pathway (1084231); Energy Metabolism Pathway (198907); Mitochondrial Biogenesis Pathway (1127495); Organelle Biogenesis And Maintenance Pathway (1127494); Signaling Events Mediated By HDAC Class I Pathway (137997); Signaling Events Mediated By HDAC Class III Pathway (137972); Transcriptional Activation Of Mitochondrial Biogenesis Pathway (1127497)

ncbi summary :

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two alternatively spliced transcript variants that encode different proteins have been described for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

SIRT3: a soluble mitochondrial NAD-dependent protein deacetylase. Can deacetylate and thereby activate a central metabolic regulator in the mitochondrial matrix, glutamate dehydrogenase. Mitochondrial proteins are hyperacetylated in SIRT3-deficient mice, but not in SIRT4 or SIRT5 deficient mice. SIRT3-deficient mice have no obvious metabolic phenotype, and the process of adaptive thermogenesis is normal. Can deacetylate and activate isocitrate dehydrogenase 2, an enzyme that participates in regulation of the citric acid cycle and the regeneration of antioxidants. Inhibited by nicotinamide, but not by trichostatin A. Its unprocessed form is enzymatically inactive. It is processed by mitochondrial processing peptidase (MPP) in the mitochondrial matrix to give an enzymatically active 28 kDa product. Protein type: Deacetylase; Mitochondrial; EC 3.5.1.-. Chromosomal Location of Human Ortholog: 11p15.5. Cellular Component: mitochondrion; membrane; mitochondrial matrix. Molecular Function: protein binding; enzyme binding; NAD-dependent histone deacetylase activity (H3-K14 specific); zinc ion binding; NAD+ ADP-ribosyltransferase activity. Biological Process: mitochondrion organization and biogenesis; protein amino acid ADP-ribosylation; protein amino acid deacetylation; organelle organization and biogenesis; aerobic respiration; aging

size1 :

0.002 mg

price1 :

140 USD

size2 :

0.01 mg

price2 :

205

size3 :

1 mg

price3 :

5015

more info or order :

MyBioSource product webpage