Recombinant Human Nudix Type Motif 1 | MyBioSource MBS144300 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human Nudix Type Motif 1

catalog :

MBS144300

quantity :

0.005 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS144300

products type :

Recombinant Protein

products full name :

Recombinant Human Nudix Type Motif 1

products short name :

Nudix Type Motif 1

products name syn :

NUDT1 Human; Nudix Type Motif 1 Human Recombinant; 7; 8-dihydro-8-oxoguanine triphosphatase; 8-oxo-dGTPase; Nucleoside diphosphate-linked moiety X motif 1; Nudix motif 1; NUDT1; MTH1

other names :

7,8-dihydro-8-oxoguanine triphosphatase isoform p18; 7,8-dihydro-8-oxoguanine triphosphatase; 7,8-dihydro-8-oxoguanine triphosphatase; 2-hydroxy-dATP diphosphatase; 8-oxo-7,8-dihydrodeoxyguanosine triphosphatase; 8-oxo-7,8-dihydroguanosine triphosphatase; 8-oxo-dGTPase; mutT human homolog 1; nucleoside diphosphate-linked moiety X motif 1; nucleoside diphosphate-linked moiety X-type motif 1; nudix motif 1; nudix (nucleoside diphosphate linked moiety X)-type motif 1; 2-hydroxy-dATP diphosphatase (EC:3.6.1.56); 8-oxo-dGTPase; Nucleoside diphosphate-linked moiety X motif 1; Nudix motif 1

products gene name :

NUDT1

other gene names :

NUDT1; NUDT1; MTH1; MTH1; Nudix motif 1

uniprot entry name :

8ODP_HUMAN

host :

E Coli

sequence length :

156

sequence :

MGSSHHHHHH SSGLVPRGSH MGASRLYTLV LVLQPQRVLL GMKKRGFGAG RWNGFGGKVQ EGETIEDGAR RELQEESGLT VDALHKVGQI VFEFVGEPEL MDVHVFCTDS IQGTPVESDE MRPCWFQLDQ IPFKDMWPDD SYWFPLLLQK KKFHGYFKFQ GQDTILDYTL REVDTV.

purity :

Greater than 95.0% as determined by SDS-PAGE.

form :

The NUDT1 solution (1 mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 10% glycerol, 2mM DTT and 100mM NaCl. Sterile Filtered colorless solution.

storage stability :

NUDT1 should be stored desiccated below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

products categories :

ENZYMES; Enzymes; Nudix Type Motif

products description :

Description: NUDT1 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E Coli is a single, non-glycosylated, polypeptide chain containing 176 amino acids (1-156 a.a.) and having a molecular mass of 20.1kDa. The NUDT1 is purified by proprietary chromatographic techniques. Introduction: NUDT1 is an enzyme that hydrolyzes oxidized purine nucleoside triphosphates, such as 8-oxo-dGTP, 8-oxo-dATP, 2-hydroxy-dATP, and 2-hydroxy rATP, to monophosphates, thereby preventing misincorporation of 8-oxo-dGTP into DNA thus preventing A:T to C:G transversions. NUDT1 is found mostly in the cytoplasm, with some in the mitochondria, suggesting that it is involved in the sanitization of nucleotide pools both for nuclear and mitochondrial genomes. Moreover, NUDT1 is expressed at much higher levels in proliferating cells than in resting cells. Misincorporation of oxidized nucleoside triphosphates into DNA/RNA during replication and transcription can cause mutations which may result in carcinogenesis or neurodegeneration.

ncbi gi num :

40288274

ncbi acc num :

NP_002443.3

ncbi gb acc num :

NM_002452.3

uniprot acc num :

P36639

ncbi mol weight :

17,952 Da

ncbi pathways :

Metabolism Pathway (477135); Metabolism Of Nucleotides Pathway (106263); Phosphate Bond Hydrolysis By NUDT Proteins Pathway (730342); Oxidized GTP And DGTP Detoxification Pathway (547505); Oxidized GTP And DGTP Detoxification Pathway (545700)

ncbi summary :

Misincorporation of oxidized nucleoside triphosphates into DNA/RNA during replication and transcription can cause mutations that may result in carcinogenesis or neurodegeneration. The protein encoded by this gene is an enzyme that hydrolyzes oxidized purine nucleoside triphosphates, such as 8-oxo-dGTP, 8-oxo-dATP, 2-hydroxy-dATP, and 2-hydroxy rATP, to monophosphates, thereby preventing misincorporation. The encoded protein is localized mainly in the cytoplasm, with some in the mitochondria, suggesting that it is involved in the sanitization of nucleotide pools both for nuclear and mitochondrial genomes. Several alternatively spliced transcript variants, some of which encode distinct isoforms, have been identified. Additional variants have been observed, but their full-length natures have not been determined. A single-nucleotide polymorphism that results in the production of an additional, longer isoform (p26) has been described. [provided by RefSeq, Jul 2008]

uniprot summary :

NUDT1: Antimutagenic. Acts as a sanitizing enzyme for oxidized nucleotide pools, thus suppressing cell dysfunction and death induced by oxidative stress. Hydrolyzes 8-oxo-dGTP, 8-oxo-dATP and 2-OH-dATP, thus preventing misincorporation of oxidized purine nucleoside triphosphates into DNA and subsequently preventing A:T to C:G and G:C to T:A transversions. Able to hydrolyze also the corresponding ribonucleotides, 2-OH-ATP, 8-oxo-GTP and 8-oxo-ATP. Belongs to the Nudix hydrolase family. 4 isoforms of the human protein are produced by alternative initiation. Protein type: Hydrolase; EC 3.6.1.56; EC 3.6.1.55. Chromosomal Location of Human Ortholog: 7p22. Cellular Component: mitochondrion; mitochondrial matrix; cytoplasm; nucleus; cytosol. Molecular Function: GTPase activity; snoRNA binding; m7G(5 )pppN diphosphatase activity; ATP diphosphatase activity; metal ion binding; 8-oxo-7,8-dihydroguanine triphosphatase activity. Biological Process: DNA protection; dGTP catabolic process; dATP catabolic process; nucleobase, nucleoside and nucleotide metabolic process; purine nucleotide catabolic process; response to oxidative stress; DNA repair

size1 :

0.005 mg

price1 :

140 USD

size2 :

0.02 mg

price2 :

205

size3 :

1 mg

price3 :

2665

more info or order :

MyBioSource product webpage