Recombinant Human Cytidine Deaminase | MyBioSource MBS144281 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human Cytidine Deaminase

catalog :

MBS144281

quantity :

0.005 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS144281

products type :

Recombinant Protein

products full name :

Recombinant Human Cytidine Deaminase

products short name :

[Cytidine Deaminase]

products name syn :

[Cytidine deaminase, Cytidine aminohydrolase, CDA, CDD.]

other names :

[cytidine deaminase; Cytidine deaminase; cytidine deaminase; cytidine aminohydrolase; cytosine nucleoside deaminase; small cytidine deaminase; cytidine deaminase; Cytidine aminohydrolase]

products gene name :

[CDA]

other gene names :

[CDA; CDA; CDD; CDD]

uniprot entry name :

CDD_HUMAN

host :

Escherichia Coli

sequence length :

146

sequence :

MGSSHHHHHH SSGLVPRGSH MAQKRPACTL KPECVQQLLV CSQEAKQSAY CPYSHFPVGA ALLTQEGRIF KGCNIENACY PLGICAERTA IQKAVSEGYK DFRAIAIASD MQDDFISPCG ACRQVMREFG TNWPVYMTKP DGTYIVMTVQ ELLPSSFGPE DLQKTQ

purity :

Greater than 90.0% as determined by SDS-PAGE.

form :

The CDA solution (0.5 mg/ml) contains 20 mM Tris-HCl Buffer (pH 8.0), 1 mM DTT, 2mM EDTA, 100 mM NaCl and 40% Glycerol. Sterile Filtered colorless solution.

storage stability :

Store at 4 degree C if entire vial will be used within 2-4 weeks.Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

other info2 :

Biological Activity: Specific activity :> 3.5unit/mg. One unit of activity was defined as the amount required to deaminate 1.0 umole of cytidine per min at pH 7.5 at 25 degree C.

products categories :

ENZYMES; Enzymes; Deaminase

products description :

Description: CDA Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E Coli is a single, non-glycosylated, polypeptide chain containing 166 amino acids (1-146 a.a.) and having a molecular mass of 18.3kDa. The CDA is purified by proprietary chromatographic techniques. Introduction: Cytidine deaminase (CDA) is an enzyme that scavenges exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis. CDA is one of several deaminases responsible for maintaining the cellular pyrimidine pool. CDA also catalyzes the deamination of chemotherapeutic cytosine nucleoside analogs such as Ara-C and 5-azacytidine, which results in the loss of their cytotoxic and antitumor function. CDA can form homotetramers and is generally expressed in granulocytes. Mutations in the CDA gene are linked to decreased sensitivity to the cytosine nucleoside analogue cytosine arabinoside used in the treatment of certain childhood leukemias.

ncbi gi num :

11386157

ncbi acc num :

NP_001776.1

ncbi gb acc num :

NM_001785.2

uniprot acc num :

P32320

ncbi mol weight :

16,185 Da

ncbi pathways :

Drug Metabolism - Other Enzymes Pathway (83033); Drug Metabolism - Other Enzymes Pathway (428); Fluoropyrimidine Activity Pathway (198778); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Nucleotides Pathway (106263); Pyrimidine Metabolism Pathway (106281); Pyrimidine Metabolism Pathway (82946); Pyrimidine Metabolism Pathway (309); Pyrimidine Salvage Reactions Pathway (106284)

ncbi summary :

This gene encodes an enzyme involved in pyrimidine salvaging. The encoded protein forms a homotetramer that catalyzes the irreversible hydrolytic deamination of cytidine and deoxycytidine to uridine and deoxyuridine, respectively. It is one of several deaminases responsible for maintaining the cellular pyrimidine pool. Mutations in this gene are associated with decreased sensitivity to the cytosine nucleoside analogue cytosine arabinoside used in the treatment of certain childhood leukemias. [provided by RefSeq, Jul 2008]

uniprot summary :

CDA: This enzyme scavenge exogenous and endogenous cytidine and 2 -deoxycytidine for UMP synthesis. Belongs to the cytidine and deoxycytidylate deaminase family. Protein type: Nucleotide Metabolism - pyrimidine; Hydrolase; EC 3.5.4.5; Xenobiotic Metabolism - drug metabolism - other enzymes. Chromosomal Location of Human Ortholog: 1p36.2-p35. Cellular Component: extracellular region; cytosol. Molecular Function: protein homodimerization activity; zinc ion binding; nucleoside binding; cytidine deaminase activity. Biological Process: cell surface receptor linked signal transduction; pyrimidine base metabolic process; cytosine metabolic process; nucleobase, nucleoside and nucleotide metabolic process; pyrimidine nucleoside salvage; cytidine deamination; negative regulation of cell growth; pyrimidine salvage; negative regulation of nucleotide metabolic process; protein homotetramerization

size1 :

0.005 mg

price1 :

140 USD

size2 :

0.02 mg

price2 :

205

size3 :

1 mg

price3 :

2665

more info or order :

MyBioSource product webpage