Recombinant Human Deoxyuridine Triphosphatase | MyBioSource MBS144236 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human Deoxyuridine Triphosphatase

catalog :

MBS144236

quantity :

0.005 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS144236

products type :

Recombinant Protein

products full name :

Recombinant Human Deoxyuridine Triphosphatase

products short name :

Deoxyuridine Triphosphatase

products name syn :

DUT Human; Deoxyuridine Triphosphatase Human Recombinant; Deoxyuridine 5''-triphosphate nucleotidohydrolase mitochondrial; dUTPase; dUTP pyrophosphatase; Deoxyuridine Triphosphatase; DUT; FLJ20622

other names :

deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial isoform 1; Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial; deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial; dUTP nucleotidohydrolase; dUTP pyrophosphatase; deoxyuridine triphosphatase; dUTP pyrophosphatase

products gene name :

DUT

other gene names :

DUT; DUT; dUTPase; dUTPase

uniprot entry name :

DUT_HUMAN

host :

E Coli

sequence length :

252

sequence :

MGSSHHHHHH SSGLVPRGSH MASTVGAAGW KGELPKAGGS PAPGPETPAI SPSKRARPAE VGGMQLRFAR LSEHATAPTR GSARAAGYDL YSAYDYTIPP MEKAVVKTDI QIALPSGCYG RVAPRSGLAA KHFIDVGAGV IDEDYRGNVG VVLFNFGKEK FEVKKGDRIA QLICERIFYP EIEEVQALDD TERGSGGFGS TGKN.

purity :

Greater than 90.0% as determined by SDS-PAGE.

form :

The DUT solution (1 mg/ml) contains 20mM Tris-HCl buffer(pH 8.0), 10% glycerol, 1mM DTT and 0.1M NaCl. Sterile Filtered colorless solution.

storage stability :

Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

products categories :

ENZYMES; Enzymes; Phosphatase

products description :

Description: DUT Human Recombinant fused with a 21 amino acid His tag at N-terminus produced in E Coli is a single, non-glycosylated, polypeptide chain containing 204 amino acids (70-252 a.a.) and having a molecular mass of 21.6kDa. The DUT is purified by proprietary chromatographic techniques. Introduction: Deoxyuridine Triphosphatase (DUT) is a ubiquitous enzyme that functions in nucleotide metabolism. Deoxyuridine Triphosphatase, in the presence of magnesium ions, is responsible for hydrolyzing dUTP to dUMP and diphosphate. This reaction is imperative for keeping the intracellular dUTP concentration low so that uracil does not become incorporated into DNA. Extensive integration of uracil into DNA can eventually lead to cell death. This suggests that DUT is crucial for cell viability, further implying that it is a prospective target for anticancer therapy.

ncbi gi num :

70906441

ncbi acc num :

NP_001020419.1

ncbi gb acc num :

NM_001025248.1

uniprot acc num :

P33316

ncbi mol weight :

17,748 Da

ncbi pathways :

Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Nucleotides Pathway (106263); Pyrimidine Biosynthesis Pathway (161056); Pyrimidine Metabolism Pathway (106281); Pyrimidine Metabolism Pathway (82946); Pyrimidine Metabolism Pathway (309); Pyrimidine Deoxyribonucleotides De Novo Biosynthesis Pathway (782380); Pyrimidine Deoxyribonucleotides De Novo Biosynthesis I Pathway (907957); Superpathway Of Pyrimidine Deoxyribonucleotides De Novo Biosynthesis (782390)

ncbi summary :

This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19. [provided by RefSeq, Jul 2008]

uniprot summary :

dUTPase: This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA. Belongs to the dUTPase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Mitochondrial; Nuclear receptor co-regulator; Motility/polarity/chemotaxis; DNA replication; Nucleotide Metabolism - pyrimidine; EC 3.6.1.23; Hydrolase. Chromosomal Location of Human Ortholog: 15q21.1. Cellular Component: nucleoplasm; mitochondrion; nucleus. Molecular Function: protein binding; magnesium ion binding; dUTP diphosphatase activity. Biological Process: dUTP catabolic process; pyrimidine base metabolic process; nucleobase, nucleoside and nucleotide metabolic process; pyrimidine nucleoside biosynthetic process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; DNA replication; dUMP biosynthetic process

size1 :

0.005 mg

price1 :

140 USD

size2 :

0.02 mg

price2 :

205

size3 :

1 mg

price3 :

2665

more info or order :

MyBioSource product webpage