protein

Recombinant Human Bleomycin Hydrolase

MBS144228

0.005 mg

140 USD

Recombinant Protein

Recombinant Human Bleomycin Hydrolase

Bleomycin Hydrolase

BLMH Human; Bleomycin Hydrolase Human Recombinant; BMH; BH; BLM hydrolase; Bleomycin Hydrolase

bleomycin hydrolase; Bleomycin hydrolase; bleomycin hydrolase; BLM hydrolase; bleomycin hydrolase

BLMH

BLMH; BLMH; BH; BMH; BH; BLM hydrolase; BMH

BLMH_HUMAN

E Coli

455

MGSSHHHHHH SSGLVPRGSH MSSSGLNSEK VAALIQKLNS DPQFVLAQNV GTTHDLLDIC LKRATVQRAQ HVFQHAVPQE GKPITNQKSS GRCWIFSCLN VMRLPFMKKL NIEEFEFSQS YLFFWDKVER CYFFLSAFVD TAQRKEPEDG RLVQFLLMNP ANDGGQWDML VNIVEKYGVI PKKCFPESYT TEATRRMNDI LNHKMREFCI RLRNLVHSGA TKGEISATQD VMMEEIFRVV CICLGNPPET FTWEYRDKDK NYQKIGPITP LEFYREHVKP LFNMEDKICL VNDPRPQHKY NKLYTVEYLS NMVGGRKTLY NNQPIDFLKK MVAASIKDGE AVWFGCDVGK HFNSKLGLSD MNLYDHELVF GVSLKNMNKA ERLTFGESLM THAMTFTAVS EKDDQDGAFT KWRVENSWGE DHGHKGYLCM TDEWFSEYVY EVVVDRKHVP EEVLAVLEQE PIILPAWDPM GALAE

Greater than 90% as determined by SDS-PAGE.

The BLMH protein solution (1mg/1ml) is formulated in 20mM Tris-HCl Buffer (pH 8.0) and 10% Glycerol. Sterile Filtered clear solution.

Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time.Please avoid freeze thaw cycles.

Biological Activity: Specific activity: > 1,000 pmole/min/ug. Measured by the hydrolysis of Met-AMC at pH 7.5, at 37C.

ENZYMES; Enzymes; Hydrolase

Description: BLMH produced in E Coli is a single, non-glycosylated polypeptide chain containing 475 amino acids (1-455a.a.) and having a molecular mass of 54.7kDa.BLMH is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. Introduction: BLMH is affiliate to the papain superfamily of the cysteine protease and the peptidase C1 family. BLMH is a cytoplasmic cysteinepeptidase usually found as a homohexamer. The standard physiological role of BLMH has not been determined, but it shields normal and malignant cells from the glycopeptide antitumor drug BLM. BLMH catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxyamide bond of its B-aminoalaninamide moiety and in addition demonstrates general aminopeptidase activity.

4557367

NP_000377.1

NM_000386.3

Q13867

52,562 Da

Adaptive Immune System Pathway (366160); Antigen Processing: Ubiquitination Proteasome Degradation Pathway (366162); Class I MHC Mediated Antigen Processing Presentation Pathway (366161); Immune System Pathway (106386)

Bleomycin hydrolase (BMH) is a cytoplasmic cysteine peptidase that is highly conserved through evolution; however, the only known activity of the enzyme is metabolic inactivation of the glycopeptide bleomycin (BLM), an essential component of combination chemotherapy regimens for cancer. The protein contains the signature active site residues of the cysteine protease papain superfamily. [provided by RefSeq, Jul 2008]

BLMH: The normal physiological role of BLM hydrolase is unknown, but it catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxamide bond of its B- aminoalaninamide moiety thus protecting normal and malignant cells from BLM toxicity. Belongs to the peptidase C1 family. Protein type: EC 3.4.22.40; Protease. Chromosomal Location of Human Ortholog: 17q11.2. Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus. Molecular Function: protein binding; carboxypeptidase activity; cysteine-type endopeptidase activity; aminopeptidase activity; cysteine-type peptidase activity. Biological Process: response to drug; antigen processing and presentation of peptide antigen via MHC class I; protein polyubiquitination; response to toxin; proteolysis. Disease: Alzheimer Disease

0.005 mg

140 USD

0.02 mg

205

1 mg

2665