Recombinant Human GTPase Activating Protein (SH3 domain) Binding Protein 1 | MyBioSource MBS144219 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human GTPase Activating Protein (SH3 domain) Binding Protein 1

catalog :

MBS144219

quantity :

0.002 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS144219

products type :

Recombinant Protein

products full name :

Recombinant Human GTPase Activating Protein (SH3 domain) Binding Protein 1

products short name :

GTPase Activating Protein

products name syn :

G3BP1 Human; GTPase Activating Protein (SH3 domain) Binding Protein 1 Human Recombinant; Ras GTPase-activating protein-binding protein 1; G3BP-1; ATP-dependent DNA helicase VIII; hDH VIII; GAP SH3 domain-binding protein 1; G3BP1; G3BP; HDH-VIII; MGC111040

other names :

ras GTPase-activating protein-binding protein 1; Ras GTPase-activating protein-binding protein 1; ras GTPase-activating protein-binding protein 1; ATP-dependent DNA helicase VIII; G3BP-1; GAP SH3 domain-binding protein 1; GAP binding protein; Ras-GTPase-activating protein SH3-domain-binding protein; RasGAP-associated endoribonuclease G3BP; GTPase activating protein (SH3 domain) binding protein 1; ATP-dependent DNA helicase VIII; hDH VIII; GAP SH3 domain-binding protein 1

products gene name :

G3BP1

other gene names :

G3BP1; G3BP1; G3BP; HDH-VIII; G3BP; G3BP-1; hDH VIII

uniprot entry name :

G3BP1_HUMAN

host :

E Coli

sequence length :

466

purity :

Greater than 85.0% as determined by SDS-PAGE.

form :

The G3BP1 solution (0.5 mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 10% glycerol, 2mM DTT and 100mM NaCl. Sterile Filtered colorless solution.

storage stability :

Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

products categories :

ENZYMES; Enzymes

products description :

Description: G3BP1 Human Recombinant fused with an 8 amino acid His tag at C-terminus produced in E Coli is a single, non-glycosylated, polypeptide chain containing 474 amino acids (1-466 a.a.) and having a molecular mass of 53.2kDa.The G3BP1 is purified by proprietary chromatographic techniques. Introduction: G3BP1 belongs to the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. G3BP1 is one of the DNA-unwinding enzymes that favors partially unwound 3'-tailed substrates and is also able to unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. G3BP1 binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. In addition, G3BP1 cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR.

ncbi gi num :

5031703

ncbi acc num :

NP_005745.1

ncbi gb acc num :

NM_005754.2

uniprot acc num :

Q13283

ncbi mol weight :

13,874 Da

ncbi summary :

This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]

uniprot summary :

G3BP-1: an hnRNA-binding protein and endoribonuclease that participates in the Ras signal transduction pathway. A regulated effector of stress granule (SG) assembly. SGs are involved in mRNA sorting in the storing of untranslated mRNAs. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3 -UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3 tail or hanging tails at both 5 - and 3 -ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5 to 3 direction along the bound single-stranded DNA. Binds to the SH3 domain of Ras GTPase-activating protein (RasGAP) in proliferating cells but not in quiescent cells. Cytoplasmic in proliferating cells, can be recruited to the plasma membrane in exponentially growing cells. Cytosolic and partially nuclear in resting cells. Recruited to SGs upon either arsenite or high temperature treatment. RasGAP-dependent phosphorylation of S149 induces a conformational change that prevents self-association. Dephosphorylation after HRAS activation is required for stress granule assembly. S149 phosphorylation induces partial nuclear localization. A component of a TAU mRNP complex, Interacts with USP10, and may regulate it. Protein type: EC 3.6.4.12; EC 3.6.4.13; Adaptor/scaffold; RNA-binding; Helicase. Chromosomal Location of Human Ortholog: 5q33.1. Cellular Component: focal adhesion; stress granule; cytoplasm; plasma membrane; nucleus; cytosol. Molecular Function: mRNA binding; ATP-dependent DNA helicase activity; protein binding; DNA binding; endonuclease activity; ATP-dependent RNA helicase activity; ATP binding. Biological Process: transport; metabolic process; Ras protein signal transduction; DNA duplex unwinding

size1 :

0.002 mg

price1 :

140 USD

size2 :

0.01 mg

price2 :

205

size3 :

1 mg

price3 :

5015

more info or order :

MyBioSource product webpage