Recombinant Human Cullin-1 | MyBioSource MBS144201 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human Cullin-1

catalog :

MBS144201

quantity :

0.002 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS144201

products type :

Recombinant Protein

products full name :

Recombinant Human Cullin-1

products short name :

Cullin-1

products name syn :

CUL1 Human; Cullin-1 Human Recombinant; Cullin 1; CUL-1; Cullin-1

other names :

cullin-1; Cullin-1; cullin-1; CUL-1; cullin 1

products gene name :

CUL1

other gene names :

CUL1; CUL1; CUL-1

uniprot entry name :

CUL1_HUMAN

host :

E Coli

sequence length :

776

sequence :

MGSSHHHHHH SSGLVPRGSH MSSTRSQNPH GLKQIGLDQI WDDLRAGIQQ VYTRQSMAKS RYMELYTHVY NYCTSVHQSN QARGAGVPPS KSKKGQTPGG AQFVGLELYK RLKEFLKNYL TNLLKDGEDL MDESVLKFYT QQWEDYRFSS KVLNGICAYL NRHWVRRECD EGRKGIYEIY SLALVTWRDC LFRPLNKQVT NAVLKLIEKE RNGETINTRL ISGVVQSYVE LGLNEDDAFA KGPTLTVYKE SFESQFLADT ERFYTRESTE FLQQNPVTEY MKKAEARLLE EQRRVQVYLH ESTQDELARK CEQVLIEKHL EIFHTEFQNL LDADKNEDLG RMYNLVSRIQ DGLGELKKLL ETHIHNQGLA AIEKCGEAAL NDPKMYVQTV LDVHKKYNAL VMSAFNNDAG FVAALDKACG RFINNNAVTK

purity :

Greater than 95% as determined by SDS-PAGE.

form :

The CUL1 protein solution (1mg/1ml) is formulated in 20mM Tris-HCl buffer (pH8.0) 1mM DTT, 100mM NaCl and 10% glycerol. Sterile Filtered clear solution.

storage stability :

Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time.Please avoid freeze thaw cycles.

other info1 :

Sterile filtered colorless solution

products categories :

RECOMBINANT & NATURAL PROTEINS; Recombinant Proteins

products description :

Description: CUL1 produced in E Coli is a single, non-glycosylated polypeptide chain containing 430 amino acids (1- 410a.a.) and having a molecular mass of 49.4kDa.CUL1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. Introduction: Cullin1, is an essential component of multiple cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes, that facilitate the ubiquitination of proteins which take part in cell cycle progression, signal transduction and transcription. In the SCF complex. Cullin1 assists in a rigid scaffold which organizes the SKP1-F-box protein and RBX1 subunits. Cullin1 contributes to catalysis by positioning of the substrate and the ubiquitin-conjugating enzyme.

ncbi gi num :

32307161

ncbi acc num :

NP_003583.2

ncbi gb acc num :

NM_003592.2

uniprot acc num :

Q13616

ncbi mol weight :

89,679 Da

ncbi pathways :

AMER1 Mutants Destabilize The Destruction Complex Pathway (1127621); APC Truncation Mutants Are Not K63 Polyubiquitinated Pathway (1127616); APC Truncation Mutants Have Impaired AXIN Binding Pathway (1127617); APC/C-mediated Degradation Of Cell Cycle Proteins Pathway (105825); AXIN Missense Mutants Destabilize The Destruction Complex Pathway (1127620); AXIN Mutants Destabilize The Destruction Complex, Activating WNT Signaling Pathway (1127618); Activation Of NF-kappaB In B Cells Pathway (576251); Adaptive Immune System Pathway (366160); Antigen Processing: Ubiquitination Proteasome Degradation Pathway (366162); Canonical Wnt Signaling Pathway (138032)

uniprot summary :

CUL1: a core scaffolding component of multiple cullin-RING-based SCF (SKP1- CUL1-F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. The cullin subunit serves as a rigid scaffold of SCF complexes. May contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex depends on the neddylation of the cullin subunit. Interacts with multiple F-box proteins in SCF complexes, directing the ubiquitination of many substrates. These F-box proteins and associated substrates include: BTRC and FBXW11 bind CTNNB1 and participate in Wnt signaling; FBXW11 bind phosphorylated NFKBIA; BTRC binds NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5 and probably NFKB2; SKP2 binds phosphorylated p27kip and is involved in regulation of G1/S transition; SKP2 binds ORC1, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1; FBXW7 binds cyclin E, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1; FBXW2 binds GCM1; FBXO32 binds MYOD1; FBXO7 binds BIRC2 and DLGAP5; FBXO33 binds YBX1; and Cyclin F binds CP110. Interacts with CHEK2, mediating its ubiquitination and regulating its function. Belongs to the cullin family. Protein type: Cell cycle regulation; Ubiquitin conjugating system. Chromosomal Location of Human Ortholog: 7q36.1. Cellular Component: nucleoplasm; cullin-RING ubiquitin ligase complex; SCF ubiquitin ligase complex; cytosol. Molecular Function: protein binding; ubiquitin protein ligase binding. Biological Process: circadian rhythm; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein monoubiquitination; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; Notch signaling pathway; viral reproduction; protein ubiquitination; cell proliferation; negative regulation of cell proliferation; organ morphogenesis; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle; G2/M transition of mitotic cell cycle; cell cycle arrest; G1/S transition of mitotic cell cycle

size1 :

0.002 mg

price1 :

140 USD

size2 :

0.01 mg

price2 :

205

size3 :

1 mg

price3 :

5015

more info or order :

MyBioSource product webpage