Recombinant human Fibrillarin | MyBioSource MBS144198 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant human Fibrillarin

catalog :

MBS144198

quantity :

0.005 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS144198

products type :

Recombinant Protein

products full name :

Recombinant human Fibrillarin

products short name :

[Fibrillarin]

products name syn :

[FBL Human; Fibrillarin Human Recombinant; rRNA 2'-O-methyltransferase fibrillarin; 34 kDa nucleolar scleroderma antigen; FBL; FIB1; FLRN; fibrillarin; FIB; RNU3IP1]

other names :

[rRNA 2'-O-methyltransferase fibrillarin; rRNA 2'-O-methyltransferase fibrillarin; rRNA 2'-O-methyltransferase fibrillarin; 34 kDa nucleolar scleroderma antigen; 34-kD nucleolar scleroderma antigen; RNA, U3 small nucleolar interacting protein 1; histone-glutamine methyltransferase; fibrillarin; 34 kDa nucleolar scleroderma antigen; Histone-glutamine methyltransferase]

products gene name :

[FBL]

other gene names :

[FBL; FBL; FIB; FLRN; RNU3IP1; FIB1; FLRN]

uniprot entry name :

FBRL_HUMAN

host :

E Coli

sequence length :

321

sequence :

MGSSHHHHHH SSGLVPRGSH RSM GKNVMVE PHRHEGVFIC RGKEDALVTK NLVPGESVYG EKRVSISEGD DKIEYRAWNP FRSKLAAAIL GGVDQIHIKP GAKVLYLGAA SGTTVSHVSD IVGPDGLVYA VEFSHRSGRD LINLAKKRTN IIPVIEDARH PHKYRMLIAM VDVIFADVAQ PDQTRIVALN AHTFLRNGGH FVISIKANCI DSTASAEAVF ASEVKKMQQE NMKPQEQLTL EPYERDHAVV VGVYRPPPKV KN

purity :

Greater than 85.0% as determined by SDS-PAGE.

form :

The FBL solution (0.5 mg/ml) contains Phosphate buffered saline (pH 7.4), 30% glycerol and 1 mM EDTA. Sterile Filtered colorless solution.

storage stability :

Store at 4°C if entire viall will be used within 2-4 weeks. Store frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

products categories :

ENZYMES; Enzymes

products description :

Description: FBL Human Recombinant fused with 23 amino acid His tag at N-terminus produced in E Coli is a single, non-glycosylated, polypeptide chain containing 262 amino acids (83-321 a.a.) and having a molecular mass of 28.9kDa. The FBL is purified by proprietary chromatographic techniques. Introduction: FBL is a significant small nucleolar protein in eukaryotes, which has an essential role in pre-rRNA processing during ribosomal biogenesis. Fibrillarin is a component of several ribonucleoproteins including a nucleolar small nuclear ribonucleoprotein (SnRNP) and one of the two classes of small nucleolar ribonucleoproteins (snoRNPs). Fibrillarin contains an N-terminal repetitive domain which is rich in glycine and arginine residues, like fibrillarins in other species. Fibrillarin's central region is similar to an RNA-binding domain and contains an RNP consensus sequence. FBL is linked to the U3, U8, and U13 small nuclear RNAs and is positioned in the dense fibrillar component (DFC) of the nucleolus. Antisera from roughly 8% of humans with the autoimmune disease scleroderma recognize fibrillarin.

ncbi gi num :

12056465

ncbi acc num :

NP_001427.2

ncbi gb acc num :

NM_001436.3

uniprot acc num :

P22087

ncbi pathways :

Ribosome Biogenesis In Eukaryotes Pathway (199378); Ribosome Biogenesis In Eukaryotes Pathway (199266); TNF-alpha/NF-kB Signaling Pathway (198884)

ncbi summary :

This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8% of humans with the autoimmune disease scleroderma recognize fibrillarin. [provided by RefSeq, Jul 2008]

uniprot summary :

FBL: Involved in pre-rRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2 - hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Interacts with NOLC1. Component of box C/D small nucleolar ribonucleoprotein (snoRNP) particles that contain NHP2L1, FBL, NOP5 and NOP56, plus a guide RNA. It is associated with the U3, U8, U13, X and Y small nuclear RNAs. Component of several ribosomal and nucleolar protein complexes. Interacts with PRMT5 and UTP20. Interacts with DDX5. Belongs to the methyltransferase superfamily. Fibrillarin family. Protein type: RNA processing; Methyltransferase; RNA-binding; Nucleolus; EC 2.1.1.-. Chromosomal Location of Human Ortholog: 19q13.1. Cellular Component: Cajal body; granular component; membrane; nucleolus; nucleus. Molecular Function: protein binding; RNA binding. Biological Process: osteoblast differentiation; tRNA processing; snoRNA metabolic process; rRNA processing

size1 :

0.005 mg

price1 :

140 USD

size2 :

0.02 mg

price2 :

205

size3 :

1 mg

price3 :

3515

more info or order :

MyBioSource product webpage