Recombinant Human Aconitase-1 | MyBioSource MBS144183 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human Aconitase-1

catalog :

MBS144183

quantity :

0.001 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS144183

products type :

Recombinant Protein

products full name :

Recombinant Human Aconitase-1

products short name :

Aconitase-1

products name syn :

ACO1 Human; Aconitase-1 Human Recombinant; Onitase 1 Soluble; IRP1; IREB1; IREBP; Citrate hydro-lyase; Iron regulatory protein 1; Ferritin repressor protein; Iron-responsive element-binding protein 1; ACONS; Aconitate Hydratase; EC 4.2.1.3; Aconitase

other names :

cytoplasmic aconitate hydratase; Cytoplasmic aconitate hydratase; cytoplasmic aconitate hydratase; IRE-BP 1; aconitate hydratase, cytoplasmic; citrate hydro-lyase; epididymis luminal protein 60; ferritin repressor protein; iron regulatory protein 1; iron-responsive element binding protein 1; iron-responsive element-binding protein 1; aconitase 1, soluble; Citrate hydro-lyase; Ferritin repressor protein; Iron regulatory protein 1; IRP1; Iron-responsive element-binding protein 1; IRE-BP 1

products gene name :

ACO1

other gene names :

ACO1; ACO1; IRP1; ACONS; HEL60; IREB1; IREBP; IREBP1; IREB1; Aconitase; IRP1; IRE-BP 1

uniprot entry name :

ACOC_HUMAN

host :

E Coli

sequence length :

889

purity :

Greater than 90% as determined by SDS-PAGE.

form :

The ACO1 protein solution (0.5mg/1ml) is formulated in 20mM Tris-HCl buffer (pH8.0)2mM DTT, 100mM NaCl and 10% glycerol. Sterile Filtered clear solution.

storage stability :

Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze thaw cycles.

products categories :

ENZYMES; Enzymes

products description :

Description: ACO1 produced in E Coli is a single, non-glycosylated polypeptide chain containing 912 amino acids (1-889a.a.) and having a molecular mass of 100.8kDa.ACO1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. Introduction: ACO1 has a part in an iron sensor. ACO1 catalyzes the stereo-specific isomerization of citrate to isocitrate via cis-aconitate in the tricarboxylic acid cycle, a non-redox-active process.

ncbi gi num :

507834063

ncbi acc num :

NP_001265281.1

ncbi gb acc num :

NM_001278352.1

uniprot acc num :

P21399

ncbi mol weight :

98,399 Da

ncbi pathways :

2-Oxocarboxylic Acid Metabolism Pathway (714485); 2-Oxocarboxylic Acid Metabolism Pathway (717400); Biosynthesis Of Amino Acids Pathway (790012); Biosynthesis Of Amino Acids Pathway (795174); Carbon Metabolism Pathway (814926); Carbon Metabolism Pathway (817567); Citrate Cycle (TCA Cycle) Pathway (82927); Citrate Cycle (TCA Cycle) Pathway (288); Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway (855811); Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway (468202)

ncbi summary :

The protein encoded by this gene is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alternative splicing results in multiple transcript variants [provided by RefSeq, Jan 2014]

uniprot summary :

IREB1: Iron sensor. Binds a 4Fe-4S cluster and functions as aconitase when cellular iron levels are high. Functions as mRNA binding protein that regulates uptake, sequestration and utilization of iron when cellular iron levels are low. Binds to iron-responsive elements (IRES) in target mRNA species when iron levels are low. Binding of a 4Fe-4S cluster precludes RNA binding. Belongs to the aconitase/IPM isomerase family. Protein type: Translation; Carbohydrate Metabolism - glyoxylate and dicarboxylate; Lyase; Carbohydrate Metabolism - citrate (TCA) cycle; RNA-binding; EC 4.2.1.3; Endoplasmic reticulum. Chromosomal Location of Human Ortholog: 9p21.1. Cellular Component: Golgi apparatus; mitochondrion; endoplasmic reticulum; cytoplasm; cytosol. Molecular Function: protein binding; iron-responsive element binding; RNA binding; metal ion binding; 4 iron, 4 sulfur cluster binding; aconitate hydratase activity. Biological Process: regulation of translation; cellular iron ion homeostasis; tricarboxylic acid cycle; citrate metabolic process; response to iron(II) ion; intestinal absorption; post-embryonic development

size1 :

0.001 mg

price1 :

140 USD

size2 :

0.005 mg

price2 :

205

size3 :

0.05 mg

price3 :

1220

more info or order :

MyBioSource product webpage