Recombinant Human Triosephosphate Isomerase 1 | MyBioSource MBS144173 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human Triosephosphate Isomerase 1

catalog :

MBS144173

quantity :

0.005 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS144173

products type :

Recombinant Protein

products full name :

Recombinant Human Triosephosphate Isomerase 1

products short name :

[Triosephosphate Isomerase 1]

products name syn :

[TPI1 Human; Triosephosphate Isomerase 1 Human Recombinant; TPI; TIM; Triosephosphate Isomerase 1]

other names :

[triosephosphate isomerase isoform 1; Triosephosphate isomerase; triosephosphate isomerase; epididymis secretory protein Li 49; triose-phosphate isomerase; triosephosphate isomerase 1; Triose-phosphate isomerase]

products gene name :

[TPI1]

other gene names :

[TPI1; TPI1; TIM; TPI; TPID; HEL-S-49; TPI; TIM]

uniprot entry name :

TPIS_HUMAN

host :

E Coli

sequence length :

249

sequence :

MGSSHHHHHH SSGLVPRGSH MAPSRKFFVG GNWKMNGRKQ SLGELIGTLN AAKVPADTEV VCAPPTAYID FARQKLDPKI AVAAQNCYKV TNGAFTGEIS PGMIKDCGAT WVVLGHSERR HVFGESDELI GQKVAHALAE GLGVIACIGE KLDEREAGIT EKVVFEQTKV IADNVKDWSK VVLAYEPVWA IGTGKTATPQ QAQEVHEKLR GWLKSNVSDA VAQSTRIIYG GSVTGATCKE LASQPDVDGF LVGGASLKPE FVDIINAKQ

purity :

Greater than 95% as determined by SDS-PAGE.

form :

The TPI1 protein solution (0.5mg/1ml) is formulated in 20mM Tris-HCl buffer (pH8.0), 1mM DTT and 10% glycerol.

concentration :

0.5mg/1ml

storage stability :

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

other info1 :

Physical Appearance: Sterile Filtered clear solution.

products categories :

ENZYMES; Enzymes; Isomerase

products description :

Description: TPI1 produced in E Coli is a single, non-glycosylated polypeptide chain containing 269 amino acids (1-249a.a.) and having a molecular mass of 28.8kDa.TPI1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. Introduction: TPI1 is one of the triosephosphate isomerase family. TPI1 catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Mutations in TPI1 causes triosephosphate isomerase deficiency (TPI deficiency). TPI deficiency is an autosomal recessive disorder which is the most severe clinical disorder of glycolysis and is related to neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection.

ncbi gi num :

4507645

ncbi acc num :

NP_000356.1

ncbi gb acc num :

NM_000365.5

uniprot acc num :

P60174

ncbi pathways :

Biosynthesis Of Amino Acids Pathway (790012); Biosynthesis Of Amino Acids Pathway (795174); Carbon Metabolism Pathway (814926); Carbon Metabolism Pathway (817567); Disease Pathway (530764); Fatty Acid Beta Oxidation Pathway (198865); Fructose And Mannose Metabolism Pathway (82930); Fructose And Mannose Metabolism Pathway (291); Gluconeogenesis Pathway (106204); Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway (413342)

ncbi summary :

This gene encodes an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Mutations in this gene are associated with triosephosphate isomerase deficiency. Pseudogenes have been identified on chromosomes 1, 4, 6 and 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]

uniprot summary :

TPI1: Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency). TPI deficiency is an autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection. Belongs to the triosephosphate isomerase family. 2 isoforms of the human protein are produced by alternative promoter. Protein type: Carbohydrate Metabolism - inositol phosphate; EC 5.3.1.1; Isomerase; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - fructose and mannose. Chromosomal Location of Human Ortholog: 12p13. Cellular Component: extracellular space; nucleus; cytosol. Molecular Function: protein binding; triose-phosphate isomerase activity. Biological Process: pentose-phosphate shunt; glycolysis; multicellular organismal development; carbohydrate metabolic process; glucose metabolic process; pathogenesis; gluconeogenesis. Disease: Triosephosphate Isomerase Deficiency

size1 :

0.005 mg

price1 :

140 USD

size2 :

0.025 mg

price2 :

205

size3 :

1 mg

price3 :

2665

more info or order :

MyBioSource product webpage