catalog number :
MBS144150
products type :
Recombinant Protein
products full name :
Recombinant Human Adenosine Deaminase, tRNA-specific 2
products short name :
Adenosine Deaminase, tRNA-specific 2
products name syn :
ADAT2 Human; Adenosine Deaminase, tRNA-specific 2 Human Recombinant; DEADC1; TAD2
other names :
tRNA-specific adenosine deaminase 2 isoform b; tRNA-specific adenosine deaminase 2; tRNA-specific adenosine deaminase 2; adenosine deaminase, tRNA-specific 2, TAD2 homolog; deaminase domain containing 1; deaminase domain-containing protein 1; tRNA-specific adenosine deaminase 2 homolog; tRNA-specific adenosine-34 deaminase subunit ADAT2; adenosine deaminase, tRNA-specific 2; Deaminase domain-containing protein 1; tRNA-specific adenosine-34 deaminase subunit ADAT2
products gene name :
ADAT2
other gene names :
ADAT2; ADAT2; TAD2; DEADC1; dJ20N2; dJ20N2.1; DEADC1
uniprot entry name :
ADAT2_HUMAN
sequence :
MGSSHHHHHH SSGLVPRGSH MEAKAAPKPA ASGACSVSAE ETEKWMEEAM HMAKEALENT EVPVGCLMVY NNEVVGKGRN EVNQTKNATR HAEMVAIDQV LDWCRQSGKS PSEVFEHTVL YVTVEPCIMC AAALRLMKIP LVVYGCQNER FGGCGSVLNI ASADLPNTGR PFQCIPGYRA EEAVEMLKTF YKQENPNAPK SKVRKKECQK S.
purity :
Greater than 90% as determined by Analysis by SDS-PAGE.
form :
The ADAT2 (0.5mg/ml) protein contains 20mM Tris-HCl buffer pH-8, 2mM DTT, 50mM NaCl and 10% glycerol. Sterile filtered colorless solution.
storage stability :
Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
products categories :
ENZYMES; Enzymes; Deaminase
products description :
Description: Recombinant Human ADAT2 produced in E Coli is a single, non-glycosylated polypeptide chain containing 211 amino acids (1-191 a.a) and having a molecular mass of 23.2 kDa. ADAT2 is fused to a 20 amino acid His Tag at N-terminus and purified by proprietary chromatographic techniques. Introduction: Adenosine Deaminase (ADA), also recognized as ADAT2 is an enzyme that takes part in purine metabolism. ADAT2 is necessary for the breakdown of adenosine from food and for the turnover of nucleic acids in tissues. ADAT2 is involved in the deamination of adenosine-34 to inosine in numerous tRNAs. ADAT2 is part of the cytidine and deoxycytidylate deaminase protein family, ADAT2 employs zinc as a cofactor. ADAT2 is a 191 amino acid enzyme that exists as two isoforms formed by alternative splicing events.
ncbi acc num :
NP_001273188.1
ncbi gb acc num :
NM_001286259.1
ncbi mol weight :
16,100 Da
uniprot summary :
ADAT2: Probably participates in deamination of adenosine-34 to inosine in many tRNAs. Belongs to the cytidine and deoxycytidylate deaminase family. ADAT2 subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Hydrolase; EC 3.5.4.-. Chromosomal Location of Human Ortholog: 6q24.2. Molecular Function: tRNA-specific adenosine deaminase activity; zinc ion binding. Biological Process: tRNA wobble adenosine to inosine editing